Positive staining for cellulose in oral pulse granuloma

Sirke Virkkunen; Henrik Wolff; Caj Haglund; Casper Højgaard; Jakob R. Winther; Martin Willemoës; Ulla Vogel; Jaana Hagström

doi:10.1016/j.oooo.2016.11.022

Positive staining for cellulose in oral pulse granuloma

Sirke Virkkunen, Henrik Wolff, Caj Haglund, Casper Højgaard, Jakob R. Winther, Martin Willemoës, Ulla Vogel, Jaana Hagström^*

^*Corresponding author for this work

Biomolecular Sciences

2 Citations (Scopus)

Abstract

Objective: Oral pulse granuloma (OPG) is an oral inflammatory lesion characterized by the presence of hyaline rings with numerous multinucleated giant cells. The etiopathogenesis of this lesion is thus far unclear, as is the composition of the hyaline rings. Our aim was to investigate whether the hyaline rings contain cellulose. Study Design: Using a newly developed staining method for cellulose, we studied 18 histologic samples diagnosed as OPG, in addition to 3 samples originally diagnosed as "normal" foreign body reactions. In our study, visualization of cellulose is based on its specific binding to the carbohydrate binding module of β-1,4-glycanase. Results: All samples diagnosed as OPG were positive for cellulose staining localized in hyaline rings. In addition, 1 lesion (of 3), first diagnosed as a foreign body reaction without the presence of hyaline rings, was positive for cellulose by horseradish peroxidase staining. Conclusions: We show for the first time that cellulose is present in OPG lesions, indicating that cellulose might be the initial cause of formation of these lesions.

Original language	English
Journal	Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Volume	123
Issue number	4
Pages (from-to)	464-467
Number of pages	4
ISSN	2212-4403
DOIs	https://doi.org/10.1016/j.oooo.2016.11.022
Publication status	Published - 1 Apr 2017

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10.1016/j.oooo.2016.11.022

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title = "Positive staining for cellulose in oral pulse granuloma",

abstract = "Objective: Oral pulse granuloma (OPG) is an oral inflammatory lesion characterized by the presence of hyaline rings with numerous multinucleated giant cells. The etiopathogenesis of this lesion is thus far unclear, as is the composition of the hyaline rings. Our aim was to investigate whether the hyaline rings contain cellulose. Study Design: Using a newly developed staining method for cellulose, we studied 18 histologic samples diagnosed as OPG, in addition to 3 samples originally diagnosed as {"}normal{"} foreign body reactions. In our study, visualization of cellulose is based on its specific binding to the carbohydrate binding module of β-1,4-glycanase. Results: All samples diagnosed as OPG were positive for cellulose staining localized in hyaline rings. In addition, 1 lesion (of 3), first diagnosed as a foreign body reaction without the presence of hyaline rings, was positive for cellulose by horseradish peroxidase staining. Conclusions: We show for the first time that cellulose is present in OPG lesions, indicating that cellulose might be the initial cause of formation of these lesions.",

author = "Sirke Virkkunen and Henrik Wolff and Caj Haglund and Casper H{\o}jgaard and Winther, {Jakob R.} and Martin Willemo{\"e}s and Ulla Vogel and Jaana Hagstr{\"o}m",

year = "2017",

month = apr,

day = "1",

doi = "10.1016/j.oooo.2016.11.022",

language = "English",

volume = "123",

pages = "464--467",

journal = "Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology",

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TY - JOUR

T1 - Positive staining for cellulose in oral pulse granuloma

AU - Virkkunen, Sirke

AU - Wolff, Henrik

AU - Haglund, Caj

AU - Højgaard, Casper

AU - Winther, Jakob R.

AU - Willemoës, Martin

AU - Vogel, Ulla

AU - Hagström, Jaana

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Objective: Oral pulse granuloma (OPG) is an oral inflammatory lesion characterized by the presence of hyaline rings with numerous multinucleated giant cells. The etiopathogenesis of this lesion is thus far unclear, as is the composition of the hyaline rings. Our aim was to investigate whether the hyaline rings contain cellulose. Study Design: Using a newly developed staining method for cellulose, we studied 18 histologic samples diagnosed as OPG, in addition to 3 samples originally diagnosed as "normal" foreign body reactions. In our study, visualization of cellulose is based on its specific binding to the carbohydrate binding module of β-1,4-glycanase. Results: All samples diagnosed as OPG were positive for cellulose staining localized in hyaline rings. In addition, 1 lesion (of 3), first diagnosed as a foreign body reaction without the presence of hyaline rings, was positive for cellulose by horseradish peroxidase staining. Conclusions: We show for the first time that cellulose is present in OPG lesions, indicating that cellulose might be the initial cause of formation of these lesions.

AB - Objective: Oral pulse granuloma (OPG) is an oral inflammatory lesion characterized by the presence of hyaline rings with numerous multinucleated giant cells. The etiopathogenesis of this lesion is thus far unclear, as is the composition of the hyaline rings. Our aim was to investigate whether the hyaline rings contain cellulose. Study Design: Using a newly developed staining method for cellulose, we studied 18 histologic samples diagnosed as OPG, in addition to 3 samples originally diagnosed as "normal" foreign body reactions. In our study, visualization of cellulose is based on its specific binding to the carbohydrate binding module of β-1,4-glycanase. Results: All samples diagnosed as OPG were positive for cellulose staining localized in hyaline rings. In addition, 1 lesion (of 3), first diagnosed as a foreign body reaction without the presence of hyaline rings, was positive for cellulose by horseradish peroxidase staining. Conclusions: We show for the first time that cellulose is present in OPG lesions, indicating that cellulose might be the initial cause of formation of these lesions.

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U2 - 10.1016/j.oooo.2016.11.022

DO - 10.1016/j.oooo.2016.11.022

M3 - Journal article

C2 - 28189527

AN - SCOPUS:85011835456

SN - 2212-4403

VL - 123

SP - 464

EP - 467

JO - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

JF - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

IS - 4

ER -

Positive staining for cellulose in oral pulse granuloma

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