Pooled human platelet lysate versus fetal bovine serum—investigating the proliferation rate, chromosome stability and angiogenic potential of human adipose tissue-derived stem cells intended for clinical use

Stig-Frederik Trojahn Kølle; Roberto S Oliveri; Peter V Glovinski; Eva Maria Kirchhoff; Anders Bruun Mathiasen; Jens Jørgen Elberg; Peter Stemann Andersen; Krzysztof Tadeusz Drzewiecki; Anne Fischer-Nielsen

doi:10.1016/j.jcyt.2013.01.217

Pooled human platelet lysate versus fetal bovine serum—investigating the proliferation rate, chromosome stability and angiogenic potential of human adipose tissue-derived stem cells intended for clinical use

Stig-Frederik Trojahn Kølle, Roberto S Oliveri, Peter V Glovinski, Eva Maria Kirchhoff, Anders Bruun Mathiasen, Jens Jørgen Elberg, Peter Stemann Andersen, Krzysztof Tadeusz Drzewiecki, Anne Fischer-Nielsen

Department of Clinical Medicine

64 Citations (Scopus)

Abstract

Background aims: Because of an increasing focus on the use of adipose-derived stem cells (ASCs) in clinical trials, the culture conditions for these cells are being optimized. We compared the proliferation rates and chromosomal stability of ASCs that had been cultured in Dulbecco's modified Eagle's Medium (DMEM) supplemented with either pooled human platelet lysate (pHPL) or clinical-grade fetal bovine serum (FBS) (DMEM^pHPL versus DMEM^FBS). Methods: ASCs from four healthy donors were cultured in either DMEM^pHPL or DMEM^FBS, and the population doubling time (PDT) was calculated. ASCs from two of the donors were expanded in DMEM^pHPL or DMEM^FBS and cultured for the final week before harvesting with or without the addition of vascular endothelial growth factor. We assessed the chromosomal stability (through the use of array comparative genomic hybridization), the expression of ASC and endothelial surface markers and the differentiation and angiogenic potential of these cells. Results: The ASCs that were cultured in pHPL exhibited a significantly shorter PDT of 29.6 h (95% confidence interval, 22.3-41.9 h) compared with those cultured in FBS, for which the PDT was 123.9 h (95% confidence interval, 95.6-176.2 h). Comparative genomic hybridization analyses revealed no chromosomal aberrations. Cell differentiation, capillary structure formation and cell-surface marker expression were generally unaffected by the type of medium supplement that was used or by the addition of vascular endothelial growth factor. Conclusions: We observed that the use of pHPL as a growth supplement for ASCs facilitated a significantly higher proliferation rate compared with FBS without compromising genomic stability or differentiation capacity.

Original language	English
Journal	Cytotherapy
Volume	15
Issue number	9
Pages (from-to)	1086-1097
Number of pages	12
ISSN	1465-3249
DOIs	https://doi.org/10.1016/j.jcyt.2013.01.217
Publication status	Published - Sept 2013

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Trojahn Kølle, S.-F., Oliveri, R. S., Glovinski, P. V., Kirchhoff, E. M., Mathiasen, A. B., Elberg, J. J., Andersen, P. S., Drzewiecki, K. T., & Fischer-Nielsen, A. (2013). Pooled human platelet lysate versus fetal bovine serum—investigating the proliferation rate, chromosome stability and angiogenic potential of human adipose tissue-derived stem cells intended for clinical use. Cytotherapy, 15(9), 1086-1097. https://doi.org/10.1016/j.jcyt.2013.01.217

Pooled human platelet lysate versus fetal bovine serum—investigating the proliferation rate, chromosome stability and angiogenic potential of human adipose tissue-derived stem cells intended for clinical use. / Trojahn Kølle, Stig-Frederik; Oliveri, Roberto S; Glovinski, Peter V et al.
In: Cytotherapy, Vol. 15, No. 9, 09.2013, p. 1086-1097.

Research output: Contribution to journal › Journal article › Research › peer-review

Trojahn Kølle, S-F, Oliveri, RS, Glovinski, PV, Kirchhoff, EM, Mathiasen, AB, Elberg, JJ, Andersen, PS, Drzewiecki, KT & Fischer-Nielsen, A 2013, 'Pooled human platelet lysate versus fetal bovine serum—investigating the proliferation rate, chromosome stability and angiogenic potential of human adipose tissue-derived stem cells intended for clinical use', Cytotherapy, vol. 15, no. 9, pp. 1086-1097. https://doi.org/10.1016/j.jcyt.2013.01.217

Trojahn Kølle SF, Oliveri RS, Glovinski PV, Kirchhoff EM, Mathiasen AB, Elberg JJ et al. Pooled human platelet lysate versus fetal bovine serum—investigating the proliferation rate, chromosome stability and angiogenic potential of human adipose tissue-derived stem cells intended for clinical use. Cytotherapy. 2013 Sept;15(9):1086-1097. doi: 10.1016/j.jcyt.2013.01.217

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title = "Pooled human platelet lysate versus fetal bovine serum—investigating the proliferation rate, chromosome stability and angiogenic potential of human adipose tissue-derived stem cells intended for clinical use",

abstract = "Background aims: Because of an increasing focus on the use of adipose-derived stem cells (ASCs) in clinical trials, the culture conditions for these cells are being optimized. We compared the proliferation rates and chromosomal stability of ASCs that had been cultured in Dulbecco's modified Eagle's Medium (DMEM) supplemented with either pooled human platelet lysate (pHPL) or clinical-grade fetal bovine serum (FBS) (DMEMpHPL versus DMEMFBS). Methods: ASCs from four healthy donors were cultured in either DMEMpHPL or DMEMFBS, and the population doubling time (PDT) was calculated. ASCs from two of the donors were expanded in DMEMpHPL or DMEMFBS and cultured for the final week before harvesting with or without the addition of vascular endothelial growth factor. We assessed the chromosomal stability (through the use of array comparative genomic hybridization), the expression of ASC and endothelial surface markers and the differentiation and angiogenic potential of these cells. Results: The ASCs that were cultured in pHPL exhibited a significantly shorter PDT of 29.6 h (95% confidence interval, 22.3-41.9 h) compared with those cultured in FBS, for which the PDT was 123.9 h (95% confidence interval, 95.6-176.2 h). Comparative genomic hybridization analyses revealed no chromosomal aberrations. Cell differentiation, capillary structure formation and cell-surface marker expression were generally unaffected by the type of medium supplement that was used or by the addition of vascular endothelial growth factor. Conclusions: We observed that the use of pHPL as a growth supplement for ASCs facilitated a significantly higher proliferation rate compared with FBS without compromising genomic stability or differentiation capacity.",

author = "{Trojahn K{\o}lle}, Stig-Frederik and Oliveri, {Roberto S} and Glovinski, {Peter V} and Kirchhoff, {Eva Maria} and Mathiasen, {Anders Bruun} and Elberg, {Jens J{\o}rgen} and Andersen, {Peter Stemann} and Drzewiecki, {Krzysztof Tadeusz} and Anne Fischer-Nielsen",

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doi = "10.1016/j.jcyt.2013.01.217",

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T1 - Pooled human platelet lysate versus fetal bovine serum—investigating the proliferation rate, chromosome stability and angiogenic potential of human adipose tissue-derived stem cells intended for clinical use

AU - Trojahn Kølle, Stig-Frederik

AU - Oliveri, Roberto S

AU - Glovinski, Peter V

AU - Kirchhoff, Eva Maria

AU - Mathiasen, Anders Bruun

AU - Elberg, Jens Jørgen

AU - Andersen, Peter Stemann

AU - Drzewiecki, Krzysztof Tadeusz

AU - Fischer-Nielsen, Anne

PY - 2013/9

Y1 - 2013/9

N2 - Background aims: Because of an increasing focus on the use of adipose-derived stem cells (ASCs) in clinical trials, the culture conditions for these cells are being optimized. We compared the proliferation rates and chromosomal stability of ASCs that had been cultured in Dulbecco's modified Eagle's Medium (DMEM) supplemented with either pooled human platelet lysate (pHPL) or clinical-grade fetal bovine serum (FBS) (DMEMpHPL versus DMEMFBS). Methods: ASCs from four healthy donors were cultured in either DMEMpHPL or DMEMFBS, and the population doubling time (PDT) was calculated. ASCs from two of the donors were expanded in DMEMpHPL or DMEMFBS and cultured for the final week before harvesting with or without the addition of vascular endothelial growth factor. We assessed the chromosomal stability (through the use of array comparative genomic hybridization), the expression of ASC and endothelial surface markers and the differentiation and angiogenic potential of these cells. Results: The ASCs that were cultured in pHPL exhibited a significantly shorter PDT of 29.6 h (95% confidence interval, 22.3-41.9 h) compared with those cultured in FBS, for which the PDT was 123.9 h (95% confidence interval, 95.6-176.2 h). Comparative genomic hybridization analyses revealed no chromosomal aberrations. Cell differentiation, capillary structure formation and cell-surface marker expression were generally unaffected by the type of medium supplement that was used or by the addition of vascular endothelial growth factor. Conclusions: We observed that the use of pHPL as a growth supplement for ASCs facilitated a significantly higher proliferation rate compared with FBS without compromising genomic stability or differentiation capacity.

AB - Background aims: Because of an increasing focus on the use of adipose-derived stem cells (ASCs) in clinical trials, the culture conditions for these cells are being optimized. We compared the proliferation rates and chromosomal stability of ASCs that had been cultured in Dulbecco's modified Eagle's Medium (DMEM) supplemented with either pooled human platelet lysate (pHPL) or clinical-grade fetal bovine serum (FBS) (DMEMpHPL versus DMEMFBS). Methods: ASCs from four healthy donors were cultured in either DMEMpHPL or DMEMFBS, and the population doubling time (PDT) was calculated. ASCs from two of the donors were expanded in DMEMpHPL or DMEMFBS and cultured for the final week before harvesting with or without the addition of vascular endothelial growth factor. We assessed the chromosomal stability (through the use of array comparative genomic hybridization), the expression of ASC and endothelial surface markers and the differentiation and angiogenic potential of these cells. Results: The ASCs that were cultured in pHPL exhibited a significantly shorter PDT of 29.6 h (95% confidence interval, 22.3-41.9 h) compared with those cultured in FBS, for which the PDT was 123.9 h (95% confidence interval, 95.6-176.2 h). Comparative genomic hybridization analyses revealed no chromosomal aberrations. Cell differentiation, capillary structure formation and cell-surface marker expression were generally unaffected by the type of medium supplement that was used or by the addition of vascular endothelial growth factor. Conclusions: We observed that the use of pHPL as a growth supplement for ASCs facilitated a significantly higher proliferation rate compared with FBS without compromising genomic stability or differentiation capacity.

U2 - 10.1016/j.jcyt.2013.01.217

DO - 10.1016/j.jcyt.2013.01.217

M3 - Journal article

C2 - 23602579

SN - 1465-3249

VL - 15

SP - 1086

EP - 1097

JO - Cytotherapy

JF - Cytotherapy

IS - 9

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