TY - JOUR
T1 - Phytoremediation of an aged petroleum contaminated soil using endophyte infected and non-infected grasses
AU - Soleimani, Mohsen
AU - Afyuni, Majid
AU - Hajabbasi, Mohammad A.
AU - Nourbakhsh, Farshid
AU - Sabzalian, Mohammad R.
AU - Christensen, Jan H.
PY - 2010/11
Y1 - 2010/11
N2 - Phytoremediation is a promising technique for cleaning petroleum contaminated soils. In this study, the effects of two grass species (Festuca arundinacea Schreb. and Festuca pratensis Huds.), infected (E+) and non-infected (E-) by endophytic fungi (Neotyphodium coenophialum and Neotyphodium uncinatum, respectively) on the degradation of petroleum hydrocarbons in an aged petroleum contaminated soil was investigated. Plants were grown in the soil for 7months and unplanted soil considered as control. At the end of the experiment, total and oil-degrading bacteria, dehydrogenase activity, water-soluble phenols, total petroleum hydrocarbons (TPH) and polycyclic aromatic hydrocarbons (PAHs) contents were measured in the soil. The results demonstrated that E+ plants contained more root and shoot biomass than E- plants and created higher levels of water-soluble phenols and dehydrogenase activity in the soil, while there was no significant difference in bacterial counts of planted soils. Planting stimulated total and oil-degrading bacterial numbers, dehydrogenase activity and the soil content of water-soluble phenols. Regardless of endophyte infection, PAH and TPH removal in the rhizosphere of plants were 80-84 and 64-72% respectively, whereas the removals in controls were 56 and 31%, respectively. It was revealed that TPHs in retention time range of n-alkanes with C10-C25 chain lengths and TPH were more degraded in the rhizosphere of E+ plants compared to E- ones. Thus, grasses infected with endophytic fungi could be more efficient for removal of TPH from oil-contaminated soils.
AB - Phytoremediation is a promising technique for cleaning petroleum contaminated soils. In this study, the effects of two grass species (Festuca arundinacea Schreb. and Festuca pratensis Huds.), infected (E+) and non-infected (E-) by endophytic fungi (Neotyphodium coenophialum and Neotyphodium uncinatum, respectively) on the degradation of petroleum hydrocarbons in an aged petroleum contaminated soil was investigated. Plants were grown in the soil for 7months and unplanted soil considered as control. At the end of the experiment, total and oil-degrading bacteria, dehydrogenase activity, water-soluble phenols, total petroleum hydrocarbons (TPH) and polycyclic aromatic hydrocarbons (PAHs) contents were measured in the soil. The results demonstrated that E+ plants contained more root and shoot biomass than E- plants and created higher levels of water-soluble phenols and dehydrogenase activity in the soil, while there was no significant difference in bacterial counts of planted soils. Planting stimulated total and oil-degrading bacterial numbers, dehydrogenase activity and the soil content of water-soluble phenols. Regardless of endophyte infection, PAH and TPH removal in the rhizosphere of plants were 80-84 and 64-72% respectively, whereas the removals in controls were 56 and 31%, respectively. It was revealed that TPHs in retention time range of n-alkanes with C10-C25 chain lengths and TPH were more degraded in the rhizosphere of E+ plants compared to E- ones. Thus, grasses infected with endophytic fungi could be more efficient for removal of TPH from oil-contaminated soils.
U2 - 10.1016/j.chemosphere.2010.09.034
DO - 10.1016/j.chemosphere.2010.09.034
M3 - Journal article
C2 - 20961596
SN - 0045-6535
VL - 81
SP - 1084
EP - 1090
JO - Chemosphere
JF - Chemosphere
IS - 9
ER -