Physical inactivity affects skeletal muscle insulin signaling in a birth weight-dependent manner

Brynjulf Mortensen; Martin Friedrichsen; Nicoline Resen Andersen; Amra C Alibegovic; Lise Højbjerre; Mette P Sonne; Bente Stallknecht; Flemming Dela; Jørgen Wojtaszewski; Allan Vaag

doi:10.1016/j.jdiacomp.2013.09.002

Physical inactivity affects skeletal muscle insulin signaling in a birth weight-dependent manner

Brynjulf Mortensen, Martin Friedrichsen, Nicoline Resen Andersen, Amra C Alibegovic, Lise Højbjerre, Mette P Sonne, Bente Stallknecht, Flemming Dela, Jørgen Wojtaszewski, Allan Vaag

18 Citations (Scopus)

Abstract

Aims We investigated whether physical inactivity could unmask defects in insulin and AMPK signaling in low birth weight (LBW) subjects. Methods Twenty LBW and 20 normal birth weight (NBW) subjects were investigated using the euglycemic-hyperinsulinemic clamp with excision of skeletal muscle biopsies pre and post 9 days of bed rest. Employing Western blotting, we investigated skeletal muscle Akt, AS160, GLUT4, and AMPK signaling. Results Peripheral insulin action was similar in the two groups and was decreased to the same extent post bed rest. Insulin and AMPK signaling was unaffected by bed rest in NBW individuals. LBW subjects showed decreased insulin-stimulated Akt phosphorylation and increased AMPK α1 and γ3 protein expression post bed rest. Insulin response of AS160 phosphorylation was lower in LBW subjects both pre and post bed rest. Conclusions Bed rest-induced insulin resistance is not explained by impaired muscle insulin or AMPK signaling in subjects with or without LBW. Lower muscle insulin signaling in LBW subjects post bed rest despite similar degree of insulin resistance as seen in controls may to some extent support the idea that LBW subjects are at higher risk of developing type 2 diabetes when being physically inactive.

Original language	English
Journal	Journal of Diabetes and its Complications
Volume	28
Issue number	1
Pages (from-to)	71-78
Number of pages	8
ISSN	1056-8727
DOIs	https://doi.org/10.1016/j.jdiacomp.2013.09.002
Publication status	Published - Jan 2014

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10.1016/j.jdiacomp.2013.09.002

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@article{32aae722ddfe4046aa86119c8a0ef25e,

title = "Physical inactivity affects skeletal muscle insulin signaling in a birth weight-dependent manner",

abstract = "Aims We investigated whether physical inactivity could unmask defects in insulin and AMPK signaling in low birth weight (LBW) subjects. Methods Twenty LBW and 20 normal birth weight (NBW) subjects were investigated using the euglycemic-hyperinsulinemic clamp with excision of skeletal muscle biopsies pre and post 9 days of bed rest. Employing Western blotting, we investigated skeletal muscle Akt, AS160, GLUT4, and AMPK signaling. Results Peripheral insulin action was similar in the two groups and was decreased to the same extent post bed rest. Insulin and AMPK signaling was unaffected by bed rest in NBW individuals. LBW subjects showed decreased insulin-stimulated Akt phosphorylation and increased AMPK α1 and γ3 protein expression post bed rest. Insulin response of AS160 phosphorylation was lower in LBW subjects both pre and post bed rest. Conclusions Bed rest-induced insulin resistance is not explained by impaired muscle insulin or AMPK signaling in subjects with or without LBW. Lower muscle insulin signaling in LBW subjects post bed rest despite similar degree of insulin resistance as seen in controls may to some extent support the idea that LBW subjects are at higher risk of developing type 2 diabetes when being physically inactive.",

author = "Brynjulf Mortensen and Martin Friedrichsen and Andersen, {Nicoline Resen} and Alibegovic, {Amra C} and Lise H{\o}jbjerre and Sonne, {Mette P} and Bente Stallknecht and Flemming Dela and J{\o}rgen Wojtaszewski and Allan Vaag",

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doi = "10.1016/j.jdiacomp.2013.09.002",

language = "English",

volume = "28",

pages = "71--78",

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T1 - Physical inactivity affects skeletal muscle insulin signaling in a birth weight-dependent manner

AU - Mortensen, Brynjulf

AU - Friedrichsen, Martin

AU - Andersen, Nicoline Resen

AU - Alibegovic, Amra C

AU - Højbjerre, Lise

AU - Sonne, Mette P

AU - Stallknecht, Bente

AU - Dela, Flemming

AU - Wojtaszewski, Jørgen

AU - Vaag, Allan

N1 - CURIS 2014 NEXS 007

PY - 2014/1

Y1 - 2014/1

N2 - Aims We investigated whether physical inactivity could unmask defects in insulin and AMPK signaling in low birth weight (LBW) subjects. Methods Twenty LBW and 20 normal birth weight (NBW) subjects were investigated using the euglycemic-hyperinsulinemic clamp with excision of skeletal muscle biopsies pre and post 9 days of bed rest. Employing Western blotting, we investigated skeletal muscle Akt, AS160, GLUT4, and AMPK signaling. Results Peripheral insulin action was similar in the two groups and was decreased to the same extent post bed rest. Insulin and AMPK signaling was unaffected by bed rest in NBW individuals. LBW subjects showed decreased insulin-stimulated Akt phosphorylation and increased AMPK α1 and γ3 protein expression post bed rest. Insulin response of AS160 phosphorylation was lower in LBW subjects both pre and post bed rest. Conclusions Bed rest-induced insulin resistance is not explained by impaired muscle insulin or AMPK signaling in subjects with or without LBW. Lower muscle insulin signaling in LBW subjects post bed rest despite similar degree of insulin resistance as seen in controls may to some extent support the idea that LBW subjects are at higher risk of developing type 2 diabetes when being physically inactive.

AB - Aims We investigated whether physical inactivity could unmask defects in insulin and AMPK signaling in low birth weight (LBW) subjects. Methods Twenty LBW and 20 normal birth weight (NBW) subjects were investigated using the euglycemic-hyperinsulinemic clamp with excision of skeletal muscle biopsies pre and post 9 days of bed rest. Employing Western blotting, we investigated skeletal muscle Akt, AS160, GLUT4, and AMPK signaling. Results Peripheral insulin action was similar in the two groups and was decreased to the same extent post bed rest. Insulin and AMPK signaling was unaffected by bed rest in NBW individuals. LBW subjects showed decreased insulin-stimulated Akt phosphorylation and increased AMPK α1 and γ3 protein expression post bed rest. Insulin response of AS160 phosphorylation was lower in LBW subjects both pre and post bed rest. Conclusions Bed rest-induced insulin resistance is not explained by impaired muscle insulin or AMPK signaling in subjects with or without LBW. Lower muscle insulin signaling in LBW subjects post bed rest despite similar degree of insulin resistance as seen in controls may to some extent support the idea that LBW subjects are at higher risk of developing type 2 diabetes when being physically inactive.

U2 - 10.1016/j.jdiacomp.2013.09.002

DO - 10.1016/j.jdiacomp.2013.09.002

M3 - Journal article

C2 - 24120282

SN - 1056-8727

VL - 28

SP - 71

EP - 78

JO - Journal of Diabetes and its Complications

JF - Journal of Diabetes and its Complications

IS - 1

ER -

Physical inactivity affects skeletal muscle insulin signaling in a birth weight-dependent manner

Abstract

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