TY - JOUR
T1 - Photosynthetic and transcriptional responses of the marine diatom Thalassiosira pseudonana to the combined effect of temperature stress and copper exposure
AU - Leung, Priscilla T. Y.
AU - Yi, Andy Xianliang
AU - Ip, Jack C. H.
AU - Mak, Sarah
AU - Leung, Kenneth M. Y.
PY - 2017/11/30
Y1 - 2017/11/30
N2 - A 96-h exposure experiment was conducted to elucidate the toxicity responses of the marine diatom Thalassiosira pseudonana upon exposure to different temperatures and copper (Cu) concentrations. Three Cu treatments (seawater control; 200μg/L Cu, EC50 for the yield at 25°C; and 1000μg/L Cu, EC50 for growth inhibition at 25°C) were conducted against four temperatures (10°C, 15°C, 25°C and 30°C). Growth rate and photosynthetic responses showed a significant interacting thermal-chemical effect with strong synergistic responses observed at 30°C treatments. Expression of heat shock protein (hsp) was positively modulated by increasing temperatures. Hsp 90, hsp90-2 and sit1 (related to silica shell formation) were highly expressed at 30°C under 1000μg/L Cu, while the genes encoding light harvesting proteins (3HfcpA and 3HfcpB) and silaffin precursor sil3 were significantly up-regulated at 15°C under 200μg/L Cu. Our results indicated an increase Cu toxicity to T. pseudonana under high temperature and Cu dose.
AB - A 96-h exposure experiment was conducted to elucidate the toxicity responses of the marine diatom Thalassiosira pseudonana upon exposure to different temperatures and copper (Cu) concentrations. Three Cu treatments (seawater control; 200μg/L Cu, EC50 for the yield at 25°C; and 1000μg/L Cu, EC50 for growth inhibition at 25°C) were conducted against four temperatures (10°C, 15°C, 25°C and 30°C). Growth rate and photosynthetic responses showed a significant interacting thermal-chemical effect with strong synergistic responses observed at 30°C treatments. Expression of heat shock protein (hsp) was positively modulated by increasing temperatures. Hsp 90, hsp90-2 and sit1 (related to silica shell formation) were highly expressed at 30°C under 1000μg/L Cu, while the genes encoding light harvesting proteins (3HfcpA and 3HfcpB) and silaffin precursor sil3 were significantly up-regulated at 15°C under 200μg/L Cu. Our results indicated an increase Cu toxicity to T. pseudonana under high temperature and Cu dose.
KW - Copper
KW - Heat shock protein
KW - Marine diatom
KW - Photosynthesis
KW - Thalassiosira
KW - Thermal stress
UR - http://www.scopus.com/inward/record.url?scp=85016466847&partnerID=8YFLogxK
U2 - 10.1016/j.marpolbul.2017.03.038
DO - 10.1016/j.marpolbul.2017.03.038
M3 - Journal article
C2 - 28365019
AN - SCOPUS:85016466847
SN - 0025-326X
VL - 124
SP - 938
EP - 945
JO - Marine Pollution Bulletin
JF - Marine Pollution Bulletin
IS - 2
ER -
