Phosphorus acquisition efficiency in arbuscular mycorrhizal maize is correlated with the abundance of root-external hyphae and the accumulation of transcripts encoding PHT1 phosphate transporters

Ruairidh J.H. Sawers; Simon Fiil Svane; Clement Quan; Mette Grønlund; Barbara Wozniak; Mesfin-Nigussie Gebreselassie; Eliécer González-Muñoz; Ricardo A. Chávez Montes; Ivan Baxter; Jerome Goudet; Iver Jakobsen; Uta Paszkowski

doi:10.1111/nph.14403

Phosphorus acquisition efficiency in arbuscular mycorrhizal maize is correlated with the abundance of root-external hyphae and the accumulation of transcripts encoding PHT1 phosphate transporters

Ruairidh J.H. Sawers, Simon Fiil Svane, Clement Quan, Mette Grønlund, Barbara Wozniak, Mesfin-Nigussie Gebreselassie, Eliécer González-Muñoz, Ricardo A. Chávez Montes, Ivan Baxter, Jerome Goudet, Iver Jakobsen, Uta Paszkowski

104 Citations (Scopus)

Abstract

Plant interactions with arbuscular mycorrhizal fungi have long attracted interest for their potential to promote more efficient use of mineral resources in agriculture. Their use, however, remains limited by a lack of understanding of the processes that determine the outcome of the symbiosis. In this study, the impact of host genotype on growth response to mycorrhizal inoculation was investigated in a panel of diverse maize lines. A panel of 30 maize lines was evaluated with and without inoculation with arbuscular mycorrhizal fungi. The line Oh43 was identified to show superior response and, along with five other reference lines, was characterized in greater detail in a split-compartment system, using ³³P to quantify mycorrhizal phosphorus uptake. Changes in relative growth indicated variation in host capacity to profit from the symbiosis. Shoot phosphate content, abundance of root-internal and -external fungal structures, mycorrhizal phosphorus uptake, and accumulation of transcripts encoding plant PHT1 family phosphate transporters varied among lines. Superior response in Oh43 is correlated with extensive development of root-external hyphae, accumulation of specific Pht1 transcripts and high phosphorus uptake by mycorrhizal plants. The data indicate that host genetic factors influence fungal growth strategy with an impact on plant performance.

Original language	English
Journal	New Phytologist
Volume	214
Issue number	2
Pages (from-to)	632-643
Number of pages	12
ISSN	0028-646X
DOIs	https://doi.org/10.1111/nph.14403
Publication status	Published - 1 Apr 2017

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https://www.biorxiv.org/content/early/2016/10/24/042028.full.pdfLicence: CC BY-NC-ND

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Sawers, R. J. H., Svane, S. F., Quan, C., Grønlund, M., Wozniak, B., Gebreselassie, M.-N., González-Muñoz, E., Montes, R. A. C., Baxter, I., Goudet, J., Jakobsen, I., & Paszkowski, U. (2017). Phosphorus acquisition efficiency in arbuscular mycorrhizal maize is correlated with the abundance of root-external hyphae and the accumulation of transcripts encoding PHT1 phosphate transporters. New Phytologist, 214(2), 632-643. https://doi.org/10.1111/nph.14403

Phosphorus acquisition efficiency in arbuscular mycorrhizal maize is correlated with the abundance of root-external hyphae and the accumulation of transcripts encoding PHT1 phosphate transporters. / Sawers, Ruairidh J.H.; Svane, Simon Fiil; Quan, Clement et al.
In: New Phytologist, Vol. 214, No. 2, 01.04.2017, p. 632-643.

Research output: Contribution to journal › Journal article › Research › peer-review

Sawers, RJH, Svane, SF, Quan, C, Grønlund, M, Wozniak, B, Gebreselassie, M-N, González-Muñoz, E, Montes, RAC, Baxter, I, Goudet, J, Jakobsen, I & Paszkowski, U 2017, 'Phosphorus acquisition efficiency in arbuscular mycorrhizal maize is correlated with the abundance of root-external hyphae and the accumulation of transcripts encoding PHT1 phosphate transporters', New Phytologist, vol. 214, no. 2, pp. 632-643. https://doi.org/10.1111/nph.14403

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title = "Phosphorus acquisition efficiency in arbuscular mycorrhizal maize is correlated with the abundance of root-external hyphae and the accumulation of transcripts encoding PHT1 phosphate transporters",

abstract = "Plant interactions with arbuscular mycorrhizal fungi have long attracted interest for their potential to promote more efficient use of mineral resources in agriculture. Their use, however, remains limited by a lack of understanding of the processes that determine the outcome of the symbiosis. In this study, the impact of host genotype on growth response to mycorrhizal inoculation was investigated in a panel of diverse maize lines. A panel of 30 maize lines was evaluated with and without inoculation with arbuscular mycorrhizal fungi. The line Oh43 was identified to show superior response and, along with five other reference lines, was characterized in greater detail in a split-compartment system, using 33P to quantify mycorrhizal phosphorus uptake. Changes in relative growth indicated variation in host capacity to profit from the symbiosis. Shoot phosphate content, abundance of root-internal and -external fungal structures, mycorrhizal phosphorus uptake, and accumulation of transcripts encoding plant PHT1 family phosphate transporters varied among lines. Superior response in Oh43 is correlated with extensive development of root-external hyphae, accumulation of specific Pht1 transcripts and high phosphorus uptake by mycorrhizal plants. The data indicate that host genetic factors influence fungal growth strategy with an impact on plant performance.",

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AU - Quan, Clement

AU - Grønlund, Mette

AU - Wozniak, Barbara

AU - Gebreselassie, Mesfin-Nigussie

AU - González-Muñoz, Eliécer

AU - Montes, Ricardo A. Chávez

AU - Baxter, Ivan

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AU - Jakobsen, Iver

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N2 - Plant interactions with arbuscular mycorrhizal fungi have long attracted interest for their potential to promote more efficient use of mineral resources in agriculture. Their use, however, remains limited by a lack of understanding of the processes that determine the outcome of the symbiosis. In this study, the impact of host genotype on growth response to mycorrhizal inoculation was investigated in a panel of diverse maize lines. A panel of 30 maize lines was evaluated with and without inoculation with arbuscular mycorrhizal fungi. The line Oh43 was identified to show superior response and, along with five other reference lines, was characterized in greater detail in a split-compartment system, using 33P to quantify mycorrhizal phosphorus uptake. Changes in relative growth indicated variation in host capacity to profit from the symbiosis. Shoot phosphate content, abundance of root-internal and -external fungal structures, mycorrhizal phosphorus uptake, and accumulation of transcripts encoding plant PHT1 family phosphate transporters varied among lines. Superior response in Oh43 is correlated with extensive development of root-external hyphae, accumulation of specific Pht1 transcripts and high phosphorus uptake by mycorrhizal plants. The data indicate that host genetic factors influence fungal growth strategy with an impact on plant performance.

AB - Plant interactions with arbuscular mycorrhizal fungi have long attracted interest for their potential to promote more efficient use of mineral resources in agriculture. Their use, however, remains limited by a lack of understanding of the processes that determine the outcome of the symbiosis. In this study, the impact of host genotype on growth response to mycorrhizal inoculation was investigated in a panel of diverse maize lines. A panel of 30 maize lines was evaluated with and without inoculation with arbuscular mycorrhizal fungi. The line Oh43 was identified to show superior response and, along with five other reference lines, was characterized in greater detail in a split-compartment system, using 33P to quantify mycorrhizal phosphorus uptake. Changes in relative growth indicated variation in host capacity to profit from the symbiosis. Shoot phosphate content, abundance of root-internal and -external fungal structures, mycorrhizal phosphorus uptake, and accumulation of transcripts encoding plant PHT1 family phosphate transporters varied among lines. Superior response in Oh43 is correlated with extensive development of root-external hyphae, accumulation of specific Pht1 transcripts and high phosphorus uptake by mycorrhizal plants. The data indicate that host genetic factors influence fungal growth strategy with an impact on plant performance.

U2 - 10.1111/nph.14403

DO - 10.1111/nph.14403

M3 - Journal article

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JF - New Phytologist

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Phosphorus acquisition efficiency in arbuscular mycorrhizal maize is correlated with the abundance of root-external hyphae and the accumulation of transcripts encoding PHT1 phosphate transporters

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