Phenotypic and genotypic characterizations of Campylobacter jejuni isolated from the broiler meat production process

Eglė Kudirkienė; Marianne Thorup Cohn; Richard A.  Stabler; Philippa C. R. Strong; Loreta Šernienė; Brendan W. Wren; Eva Møller Nielsen; Mindaugas Malakauskas; Lone Brøndsted

doi:10.1007/s00284-012-0170-z

Phenotypic and genotypic characterizations of Campylobacter jejuni isolated from the broiler meat production process

Eglė Kudirkienė, Marianne Thorup Cohn, Richard A. Stabler, Philippa C. R. Strong, Loreta Šernienė, Brendan W. Wren, Eva Møller Nielsen, Mindaugas Malakauskas , Lone Brøndsted

15 Citations (Scopus)

Abstract

A set of C. jejuni isolates of different origins and flaA-genotypes obtained throughout the broiler meat production chain was tested in this study for a possible correlation of their origin, phylogenetic relationship, and phenotypic properties. Interestingly, the results showed a correlation of the origin and the phylogenetic relationship between the C. jejuni isolates and their ability to form biofilm, but not in their ability to survive at -18, 5, 20, and 48 °C. Two strains, a broiler cloacae isolate and a broiler fillet isolate, were unable to develop biofilm, while most of the C. jejuni isolates originating from meat and surfaces of the slaughterhouse readily formed biofilms after both 24, 48, and 72 h. Interestingly, these biofilm-forming strains were closely related. Furthermore, two strains that were isolated after disinfection developed significantly more biofilms after 24 h of incubation than the remaining strains. A comparative genomic analysis using DNA microarrays showed that the gene contents of strains that efficiently formed biofilms were different from those that did not. The study suggests that biofilm formation might be a lineage specific property, allowing C. jejuni to both survive environmental stress at the slaughterhouse and to attach to the surface of meat.

Original language	English
Journal	Current Microbiology
Volume	65
Issue number	4
Pages (from-to)	398-406
Number of pages	9
ISSN	0343-8651
DOIs	https://doi.org/10.1007/s00284-012-0170-z
Publication status	Published - Oct 2012

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@article{9d3c38224faa4b9aad8dadbba679a2cc,

title = "Phenotypic and genotypic characterizations of Campylobacter jejuni isolated from the broiler meat production process",

abstract = "A set of C. jejuni isolates of different origins and flaA-genotypes obtained throughout the broiler meat production chain was tested in this study for a possible correlation of their origin, phylogenetic relationship, and phenotypic properties. Interestingly, the results showed a correlation of the origin and the phylogenetic relationship between the C. jejuni isolates and their ability to form biofilm, but not in their ability to survive at -18, 5, 20, and 48 °C. Two strains, a broiler cloacae isolate and a broiler fillet isolate, were unable to develop biofilm, while most of the C. jejuni isolates originating from meat and surfaces of the slaughterhouse readily formed biofilms after both 24, 48, and 72 h. Interestingly, these biofilm-forming strains were closely related. Furthermore, two strains that were isolated after disinfection developed significantly more biofilms after 24 h of incubation than the remaining strains. A comparative genomic analysis using DNA microarrays showed that the gene contents of strains that efficiently formed biofilms were different from those that did not. The study suggests that biofilm formation might be a lineage specific property, allowing C. jejuni to both survive environmental stress at the slaughterhouse and to attach to the surface of meat.",

author = "Eglė Kudirkienė and Cohn, {Marianne Thorup} and Stabler, {Richard A.} and Strong, {Philippa C. R.} and Loreta {\v S}ernienė and Wren, {Brendan W.} and Nielsen, {Eva M{\o}ller} and Mindaugas Malakauskas and Lone Br{\o}ndsted",

year = "2012",

month = oct,

doi = "10.1007/s00284-012-0170-z",

language = "English",

volume = "65",

pages = "398--406",

journal = "Current Microbiology",

issn = "0343-8651",

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T1 - Phenotypic and genotypic characterizations of Campylobacter jejuni isolated from the broiler meat production process

AU - Kudirkienė, Eglė

AU - Cohn, Marianne Thorup

AU - Stabler, Richard A.

AU - Strong, Philippa C. R.

AU - Šernienė, Loreta

AU - Wren, Brendan W.

AU - Nielsen, Eva Møller

AU - Malakauskas , Mindaugas

AU - Brøndsted, Lone

PY - 2012/10

Y1 - 2012/10

N2 - A set of C. jejuni isolates of different origins and flaA-genotypes obtained throughout the broiler meat production chain was tested in this study for a possible correlation of their origin, phylogenetic relationship, and phenotypic properties. Interestingly, the results showed a correlation of the origin and the phylogenetic relationship between the C. jejuni isolates and their ability to form biofilm, but not in their ability to survive at -18, 5, 20, and 48 °C. Two strains, a broiler cloacae isolate and a broiler fillet isolate, were unable to develop biofilm, while most of the C. jejuni isolates originating from meat and surfaces of the slaughterhouse readily formed biofilms after both 24, 48, and 72 h. Interestingly, these biofilm-forming strains were closely related. Furthermore, two strains that were isolated after disinfection developed significantly more biofilms after 24 h of incubation than the remaining strains. A comparative genomic analysis using DNA microarrays showed that the gene contents of strains that efficiently formed biofilms were different from those that did not. The study suggests that biofilm formation might be a lineage specific property, allowing C. jejuni to both survive environmental stress at the slaughterhouse and to attach to the surface of meat.

AB - A set of C. jejuni isolates of different origins and flaA-genotypes obtained throughout the broiler meat production chain was tested in this study for a possible correlation of their origin, phylogenetic relationship, and phenotypic properties. Interestingly, the results showed a correlation of the origin and the phylogenetic relationship between the C. jejuni isolates and their ability to form biofilm, but not in their ability to survive at -18, 5, 20, and 48 °C. Two strains, a broiler cloacae isolate and a broiler fillet isolate, were unable to develop biofilm, while most of the C. jejuni isolates originating from meat and surfaces of the slaughterhouse readily formed biofilms after both 24, 48, and 72 h. Interestingly, these biofilm-forming strains were closely related. Furthermore, two strains that were isolated after disinfection developed significantly more biofilms after 24 h of incubation than the remaining strains. A comparative genomic analysis using DNA microarrays showed that the gene contents of strains that efficiently formed biofilms were different from those that did not. The study suggests that biofilm formation might be a lineage specific property, allowing C. jejuni to both survive environmental stress at the slaughterhouse and to attach to the surface of meat.

U2 - 10.1007/s00284-012-0170-z

DO - 10.1007/s00284-012-0170-z

M3 - Journal article

C2 - 22735984

SN - 0343-8651

VL - 65

SP - 398

EP - 406

JO - Current Microbiology

JF - Current Microbiology

IS - 4

ER -

Phenotypic and genotypic characterizations of Campylobacter jejuni isolated from the broiler meat production process

Abstract

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