Phage display of peptide / major histocompatibility class I complexes

N Vest Hansen; L Ostergaard Pedersen; A Stryhn; S Buus

Phage display of peptide / major histocompatibility class I complexes

N Vest Hansen, L Ostergaard Pedersen, A Stryhn, S Buus

Department of Immunology and Microbiology

16 Citations (Scopus)

Abstract

Major histocompatibility complex class I (MHC-I) molecules sample peptides from the intracellular environment and present them to cytotoxic T cells (CTL). To establish a selection system, and, thereby, enable a library approach to identify the specificities involved (that of the MHC-I for peptides and subsequently that ot the T cell receptor for peptide-MHC-I complex), we have fused a single chain peptide-MHC-I complex to the phage minor coat protein, gpIII, and displayed it on filamentous phage. Expression of peptide-MHC-I complexes was shown with relevant conformation-specific monoclonal antibodies and, more importantly, with a unique "T cell receptor-like" (i. e. peptide-specific, MHC-I-restricted) antibody. Thus, properly assembled and folded peptide-MHC-I complexes can be displayed on filamentous phage. Despite the successful display, interaction with T cells could not be demonstrated.

Original language	English
Journal	European Journal of Immunology
Volume	31
Issue number	1
Pages (from-to)	32-8
Number of pages	6
ISSN	0014-2980
Publication status	Published - 2001

Cite this

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title = "Phage display of peptide / major histocompatibility class I complexes",

abstract = "Major histocompatibility complex class I (MHC-I) molecules sample peptides from the intracellular environment and present them to cytotoxic T cells (CTL). To establish a selection system, and, thereby, enable a library approach to identify the specificities involved (that of the MHC-I for peptides and subsequently that ot the T cell receptor for peptide-MHC-I complex), we have fused a single chain peptide-MHC-I complex to the phage minor coat protein, gpIII, and displayed it on filamentous phage. Expression of peptide-MHC-I complexes was shown with relevant conformation-specific monoclonal antibodies and, more importantly, with a unique {"}T cell receptor-like{"} (i. e. peptide-specific, MHC-I-restricted) antibody. Thus, properly assembled and folded peptide-MHC-I complexes can be displayed on filamentous phage. Despite the successful display, interaction with T cells could not be demonstrated.",

author = "{Vest Hansen}, N and {Ostergaard Pedersen}, L and A Stryhn and S Buus",

note = "Keywords: Amino Acid Sequence; Bacteriophages; Base Sequence; Histocompatibility Antigens Class I; Molecular Sequence Data; Peptides; Protein Folding; Receptors, Antigen, T-Cell; Recombinant Fusion Proteins",

year = "2001",

language = "English",

volume = "31",

pages = "32--8",

journal = "European Journal of Immunology",

issn = "0014-2980",

publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",

number = "1",

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TY - JOUR

T1 - Phage display of peptide / major histocompatibility class I complexes

AU - Vest Hansen, N

AU - Ostergaard Pedersen, L

AU - Stryhn, A

AU - Buus, S

N1 - Keywords: Amino Acid Sequence; Bacteriophages; Base Sequence; Histocompatibility Antigens Class I; Molecular Sequence Data; Peptides; Protein Folding; Receptors, Antigen, T-Cell; Recombinant Fusion Proteins

PY - 2001

Y1 - 2001

N2 - Major histocompatibility complex class I (MHC-I) molecules sample peptides from the intracellular environment and present them to cytotoxic T cells (CTL). To establish a selection system, and, thereby, enable a library approach to identify the specificities involved (that of the MHC-I for peptides and subsequently that ot the T cell receptor for peptide-MHC-I complex), we have fused a single chain peptide-MHC-I complex to the phage minor coat protein, gpIII, and displayed it on filamentous phage. Expression of peptide-MHC-I complexes was shown with relevant conformation-specific monoclonal antibodies and, more importantly, with a unique "T cell receptor-like" (i. e. peptide-specific, MHC-I-restricted) antibody. Thus, properly assembled and folded peptide-MHC-I complexes can be displayed on filamentous phage. Despite the successful display, interaction with T cells could not be demonstrated.

AB - Major histocompatibility complex class I (MHC-I) molecules sample peptides from the intracellular environment and present them to cytotoxic T cells (CTL). To establish a selection system, and, thereby, enable a library approach to identify the specificities involved (that of the MHC-I for peptides and subsequently that ot the T cell receptor for peptide-MHC-I complex), we have fused a single chain peptide-MHC-I complex to the phage minor coat protein, gpIII, and displayed it on filamentous phage. Expression of peptide-MHC-I complexes was shown with relevant conformation-specific monoclonal antibodies and, more importantly, with a unique "T cell receptor-like" (i. e. peptide-specific, MHC-I-restricted) antibody. Thus, properly assembled and folded peptide-MHC-I complexes can be displayed on filamentous phage. Despite the successful display, interaction with T cells could not be demonstrated.

M3 - Journal article

C2 - 11169435

SN - 0014-2980

VL - 31

SP - 32

EP - 38

JO - European Journal of Immunology

JF - European Journal of Immunology

IS - 1

ER -

Phage display of peptide / major histocompatibility class I complexes

Abstract

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