Phage display of peptide / major histocompatibility class I complexes

N Vest Hansen, L Ostergaard Pedersen, A Stryhn, S Buus

16 Citations (Scopus)

Abstract

Major histocompatibility complex class I (MHC-I) molecules sample peptides from the intracellular environment and present them to cytotoxic T cells (CTL). To establish a selection system, and, thereby, enable a library approach to identify the specificities involved (that of the MHC-I for peptides and subsequently that ot the T cell receptor for peptide-MHC-I complex), we have fused a single chain peptide-MHC-I complex to the phage minor coat protein, gpIII, and displayed it on filamentous phage. Expression of peptide-MHC-I complexes was shown with relevant conformation-specific monoclonal antibodies and, more importantly, with a unique "T cell receptor-like" (i. e. peptide-specific, MHC-I-restricted) antibody. Thus, properly assembled and folded peptide-MHC-I complexes can be displayed on filamentous phage. Despite the successful display, interaction with T cells could not be demonstrated.
Original languageEnglish
JournalEuropean Journal of Immunology
Volume31
Issue number1
Pages (from-to)32-8
Number of pages6
ISSN0014-2980
Publication statusPublished - 2001

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