Phage-display libraries of murine and human antibody Fab fragments

J Engberg; P S Andersen; L K Nielsen; Morten Hanefeld Dziegiel; L K Johansen; B Albrechtsen

doi:10.1007/BF02761708

Phage-display libraries of murine and human antibody Fab fragments

J Engberg, P S Andersen, L K Nielsen, Morten Hanefeld Dziegiel, L K Johansen, B Albrechtsen

38 Citations (Scopus)

Abstract

We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.

Original language	English
Journal	Molecular Biotechnology
Volume	6
Issue number	3
Pages (from-to)	287-310
Number of pages	24
ISSN	1073-6085
DOIs	https://doi.org/10.1007/BF02761708
Publication status	Published - Dec 1996

Keywords

Animals
Bacteriophages
Gene Library
Humans
Immunoglobulin Fab Fragments
Mice

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10.1007/BF02761708

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title = "Phage-display libraries of murine and human antibody Fab fragments",

abstract = "We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.",

keywords = "Animals, Bacteriophages, Gene Library, Humans, Immunoglobulin Fab Fragments, Mice",

author = "J Engberg and Andersen, {P S} and Nielsen, {L K} and Dziegiel, {Morten Hanefeld} and Johansen, {L K} and B Albrechtsen",

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T1 - Phage-display libraries of murine and human antibody Fab fragments

AU - Engberg, J

AU - Andersen, P S

AU - Nielsen, L K

AU - Dziegiel, Morten Hanefeld

AU - Johansen, L K

AU - Albrechtsen, B

PY - 1996/12

Y1 - 1996/12

N2 - We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.

AB - We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.

KW - Animals

KW - Bacteriophages

KW - Gene Library

KW - Humans

KW - Immunoglobulin Fab Fragments

KW - Mice

U2 - 10.1007/BF02761708

DO - 10.1007/BF02761708

M3 - Journal article

C2 - 9067975

SN - 1073-6085

VL - 6

SP - 287

EP - 310

JO - Molecular Biotechnology

JF - Molecular Biotechnology

IS - 3

ER -

Phage-display libraries of murine and human antibody Fab fragments

Abstract

Keywords

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