Peptidoglycan and muropeptides from pathogens Agrobacterium and Xanthomonas elicit plant innate immunity: structure and activity

TY - JOUR

T1 - Peptidoglycan and muropeptides from pathogens Agrobacterium and Xanthomonas elicit plant innate immunity

T2 - structure and activity

AU - Erbs, Gitte

AU - Silipo, Alba

AU - Aslam, Shazia

AU - Castro, Cristina De

AU - Liparoti, Valeria

AU - Flagiello, Angela

AU - Pucci, Pietro

AU - Lanzetta, Rosa

AU - Parrilli, Michelangelo

AU - Molinaro, Antonio

AU - Newman, Mari-Anne

AU - Cooper, Richard M.

PY - 2008

Y1 - 2008

N2 - Peptidoglycan (PGN) is a unique and essential structural part of the bacterial cell wall. PGNs from two contrasting Gram-negative plant pathogenic bacteria elicited components characteristic of the innate immune system in Arabidopsis thaliana, such as transcription of the defense gene PR1, oxidative burst, medium alkalinization, and formation of callose. Highly purified muropeptides from PGNs were more effective elicitors of early defense responses than native PGN. Therefore, PGN and its constituents represent a Microbe-Associated Molecular Pattern (MAMP) in plant-bacterial interactions. PGN and muropeptides from aggressive Xanthomonas campestris pv. campestris were significantly more active than those from Agrobacterium tumefaciens, which must maintain host cell viability during infection. The structure of muropeptide components and the distinctive differences are described. Differing defense-eliciting abilities appear to depend on subtle structural differences in either carbohydrate or peptide groups.

AB - Peptidoglycan (PGN) is a unique and essential structural part of the bacterial cell wall. PGNs from two contrasting Gram-negative plant pathogenic bacteria elicited components characteristic of the innate immune system in Arabidopsis thaliana, such as transcription of the defense gene PR1, oxidative burst, medium alkalinization, and formation of callose. Highly purified muropeptides from PGNs were more effective elicitors of early defense responses than native PGN. Therefore, PGN and its constituents represent a Microbe-Associated Molecular Pattern (MAMP) in plant-bacterial interactions. PGN and muropeptides from aggressive Xanthomonas campestris pv. campestris were significantly more active than those from Agrobacterium tumefaciens, which must maintain host cell viability during infection. The structure of muropeptide components and the distinctive differences are described. Differing defense-eliciting abilities appear to depend on subtle structural differences in either carbohydrate or peptide groups.

KW - Former LIFE faculty

KW - PROTEINS

KW - CHEMBIO

KW - MICROBIO

U2 - 10.1016/j.chembiol.2008.03.017

DO - 10.1016/j.chembiol.2008.03.017

M3 - Journal article

C2 - 18482696

SN - 1074-5521

VL - 15

SP - 438

EP - 448

JO - Chemistry & Biology

JF - Chemistry & Biology

IS - 5

ER -