@inbook{c80a8cd9291a4f878a38641f4454ce50,
title = "Peptide release, side-chain deprotection, work-up, and isolation",
abstract = "After having successfully synthesized a peptide, it has to be released from the solid support, unless it is being used for on-resin display. The linker and, in some cases, the cleavage mixture determine the C-terminal functionality of the released peptide. In most cases, the peptide is released with concomitant removal of side-chain protecting groups. However, some combinations of linkers and side-chain protecting groups enable a two-stage procedure, either using orthogonal chemistry or graduated labilities. Herein, we describe protocols for the release of peptides from the most commonly used linker types providing a variety of different C-terminal functionalities, including acids, amides, amines, and aldehydes. Moreover, suggestions for determination of peptide purity and for storage conditions are provided.",
keywords = "Peptide cleavage, Side-chain deprotection, Rink amide linker, Wang linker, Hydrazine benzoyl linker, HMBA linker",
author = "Pedersen, {S{\o}ren Ljungberg} and Jensen, {Knud J{\o}rgen}",
year = "2013",
doi = "10.1007/978-1-62703-544-6_3",
language = "English",
isbn = "978-1-62703-543-9",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "43--63",
editor = "Jensen, {Knud J.} and Shelton, {Pernille T.} and Pedersen, {S{\o}ren L.}",
booktitle = "Peptide synthesis and applications",
address = "United States",
}