PDE9A, PDE10A, and PDE11A expression in rat trigeminovascular pain signalling system

Lars S Kruse; Morten Møller; Maiken Tibaek; Steen Gammeltoft; Jes Olesen; Christina Kruuse; Lars S Kruse; Morten Møller; Maiken Tibaek; Steen Gammeltoft; Jes Olesen; Christina Rostrup Kruuse

doi:10.1016/j.brainres.2009.05.012

PDE9A, PDE10A, and PDE11A expression in rat trigeminovascular pain signalling system

Lars S Kruse, Morten Møller, Maiken Tibaek, Steen Gammeltoft, Jes Olesen, Christina Kruuse, Lars S Kruse, Morten Møller, Maiken Tibaek, Steen Gammeltoft, Jes Olesen, Christina Rostrup Kruuse

12 Citations (Scopus)

Abstract

Activation of the trigeminovascular pain signalling system, including cerebral arteries, meninges, trigeminal ganglion, and brain stem, is involved in migraine. Furthermore, stimulation of cyclic nucleotide (cAMP and cGMP) production as well as inhibition of phosphodiesterases (PDEs) induces headache and migraine. In order to investigate the possible role of PDE in the pain pathway of migraine, expression of the most recently discovered PDE subtypes (9A, 10A and 11A) in cerebral arteries, dura mater, and trigeminal ganglion and nucleus was examined. The presence of mRNA and protein in the middle cerebral artery, basilar artery, meninges, trigeminal ganglion, and spinal trigeminal nucleus of male Sprague-Dawley rats were investigated using real-time PCR, Western blot, and immunohistochemistry. The results were compared to two peripheral arteries: aorta and mesenteric artery, as well as neocortex and cerebellar cortex. Real-time PCR and Western blotting showed that PDE9A, PDE10A and PDE11A are expressed in components of the rat trigeminovascular pain signalling system including middle cerebral artery, basilar artery, meninges, trigeminal ganglion and spinal trigeminal nucleus. Aorta and mesenteric artery as well as cerebral neocortex and cerebellar cortex also showed expression of PDE9A, PDE10A and PDE11A. Immunohistochemistry revealed that PDE9A, PDE10A and PDE11A are localised in the cytosol of nerve cell bodies of the trigeminal ganglion. We here present, for the first time, the expression of PDE9A, PDE10A, and PDE11A in the trigeminovascular system. The functional implications are yet unknown, but their localisation indicates that they may have a role in the pain pathway of migraine as well as trigeminal neuralgia and trigeminal autonomic cephalalgias.

Original language	English
Journal	Brain Research
Volume	1281
Pages (from-to)	25-34
Number of pages	9
ISSN	0006-8993
DOIs	https://doi.org/10.1016/j.brainres.2009.05.012 https://doi.org/10.1016/j.brainres.2009.05.012
Publication status	Published - 2009

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Kruse, L. S., Møller, M., Tibaek, M., Gammeltoft, S., Olesen, J., Kruuse, C., Kruse, L. S., Møller, M., Tibaek, M., Gammeltoft, S., Olesen, J., & Kruuse, C. R. (2009). PDE9A, PDE10A, and PDE11A expression in rat trigeminovascular pain signalling system. Brain Research, 1281, 25-34. https://doi.org/10.1016/j.brainres.2009.05.012, https://doi.org/10.1016/j.brainres.2009.05.012

@article{60f26e00a92c11df928f000ea68e967b,

title = "PDE9A, PDE10A, and PDE11A expression in rat trigeminovascular pain signalling system",

abstract = "Activation of the trigeminovascular pain signalling system, including cerebral arteries, meninges, trigeminal ganglion, and brain stem, is involved in migraine. Furthermore, stimulation of cyclic nucleotide (cAMP and cGMP) production as well as inhibition of phosphodiesterases (PDEs) induces headache and migraine. In order to investigate the possible role of PDE in the pain pathway of migraine, expression of the most recently discovered PDE subtypes (9A, 10A and 11A) in cerebral arteries, dura mater, and trigeminal ganglion and nucleus was examined. The presence of mRNA and protein in the middle cerebral artery, basilar artery, meninges, trigeminal ganglion, and spinal trigeminal nucleus of male Sprague-Dawley rats were investigated using real-time PCR, Western blot, and immunohistochemistry. The results were compared to two peripheral arteries: aorta and mesenteric artery, as well as neocortex and cerebellar cortex. Real-time PCR and Western blotting showed that PDE9A, PDE10A and PDE11A are expressed in components of the rat trigeminovascular pain signalling system including middle cerebral artery, basilar artery, meninges, trigeminal ganglion and spinal trigeminal nucleus. Aorta and mesenteric artery as well as cerebral neocortex and cerebellar cortex also showed expression of PDE9A, PDE10A and PDE11A. Immunohistochemistry revealed that PDE9A, PDE10A and PDE11A are localised in the cytosol of nerve cell bodies of the trigeminal ganglion. We here present, for the first time, the expression of PDE9A, PDE10A, and PDE11A in the trigeminovascular system. The functional implications are yet unknown, but their localisation indicates that they may have a role in the pain pathway of migraine as well as trigeminal neuralgia and trigeminal autonomic cephalalgias.",

author = "Kruse, {Lars S} and Morten M{\o}ller and Maiken Tibaek and Steen Gammeltoft and Jes Olesen and Christina Kruuse and Kruse, {Lars S} and Morten M{\o}ller and Maiken Tibaek and Steen Gammeltoft and Jes Olesen and Kruuse, {Christina Rostrup}",

note = "Keywords: 3',5'-Cyclic-AMP Phosphodiesterases; Animals; Aorta; Blotting, Western; Brain; Cerebral Arteries; Dura Mater; Immunohistochemistry; Male; Mesenteric Arteries; Migraine Disorders; Neurons; Pain; Phosphoric Diester Hydrolases; RNA, Messenger; Rats; Rats, Sprague-Dawley; Signal Transduction; Trigeminal Ganglion; Trigeminal Nuclei; Trigeminal Nucleus, Spinal",

year = "2009",

doi = "10.1016/j.brainres.2009.05.012",

language = "English",

volume = "1281",

pages = "25--34",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier",

}

TY - JOUR

T1 - PDE9A, PDE10A, and PDE11A expression in rat trigeminovascular pain signalling system

AU - Kruse, Lars S

AU - Møller, Morten

AU - Tibaek, Maiken

AU - Gammeltoft, Steen

AU - Olesen, Jes

AU - Kruuse, Christina

AU - Kruse, Lars S

AU - Møller, Morten

AU - Tibaek, Maiken

AU - Gammeltoft, Steen

AU - Olesen, Jes

AU - Kruuse, Christina Rostrup

N1 - Keywords: 3',5'-Cyclic-AMP Phosphodiesterases; Animals; Aorta; Blotting, Western; Brain; Cerebral Arteries; Dura Mater; Immunohistochemistry; Male; Mesenteric Arteries; Migraine Disorders; Neurons; Pain; Phosphoric Diester Hydrolases; RNA, Messenger; Rats; Rats, Sprague-Dawley; Signal Transduction; Trigeminal Ganglion; Trigeminal Nuclei; Trigeminal Nucleus, Spinal

PY - 2009

Y1 - 2009

N2 - Activation of the trigeminovascular pain signalling system, including cerebral arteries, meninges, trigeminal ganglion, and brain stem, is involved in migraine. Furthermore, stimulation of cyclic nucleotide (cAMP and cGMP) production as well as inhibition of phosphodiesterases (PDEs) induces headache and migraine. In order to investigate the possible role of PDE in the pain pathway of migraine, expression of the most recently discovered PDE subtypes (9A, 10A and 11A) in cerebral arteries, dura mater, and trigeminal ganglion and nucleus was examined. The presence of mRNA and protein in the middle cerebral artery, basilar artery, meninges, trigeminal ganglion, and spinal trigeminal nucleus of male Sprague-Dawley rats were investigated using real-time PCR, Western blot, and immunohistochemistry. The results were compared to two peripheral arteries: aorta and mesenteric artery, as well as neocortex and cerebellar cortex. Real-time PCR and Western blotting showed that PDE9A, PDE10A and PDE11A are expressed in components of the rat trigeminovascular pain signalling system including middle cerebral artery, basilar artery, meninges, trigeminal ganglion and spinal trigeminal nucleus. Aorta and mesenteric artery as well as cerebral neocortex and cerebellar cortex also showed expression of PDE9A, PDE10A and PDE11A. Immunohistochemistry revealed that PDE9A, PDE10A and PDE11A are localised in the cytosol of nerve cell bodies of the trigeminal ganglion. We here present, for the first time, the expression of PDE9A, PDE10A, and PDE11A in the trigeminovascular system. The functional implications are yet unknown, but their localisation indicates that they may have a role in the pain pathway of migraine as well as trigeminal neuralgia and trigeminal autonomic cephalalgias.

AB - Activation of the trigeminovascular pain signalling system, including cerebral arteries, meninges, trigeminal ganglion, and brain stem, is involved in migraine. Furthermore, stimulation of cyclic nucleotide (cAMP and cGMP) production as well as inhibition of phosphodiesterases (PDEs) induces headache and migraine. In order to investigate the possible role of PDE in the pain pathway of migraine, expression of the most recently discovered PDE subtypes (9A, 10A and 11A) in cerebral arteries, dura mater, and trigeminal ganglion and nucleus was examined. The presence of mRNA and protein in the middle cerebral artery, basilar artery, meninges, trigeminal ganglion, and spinal trigeminal nucleus of male Sprague-Dawley rats were investigated using real-time PCR, Western blot, and immunohistochemistry. The results were compared to two peripheral arteries: aorta and mesenteric artery, as well as neocortex and cerebellar cortex. Real-time PCR and Western blotting showed that PDE9A, PDE10A and PDE11A are expressed in components of the rat trigeminovascular pain signalling system including middle cerebral artery, basilar artery, meninges, trigeminal ganglion and spinal trigeminal nucleus. Aorta and mesenteric artery as well as cerebral neocortex and cerebellar cortex also showed expression of PDE9A, PDE10A and PDE11A. Immunohistochemistry revealed that PDE9A, PDE10A and PDE11A are localised in the cytosol of nerve cell bodies of the trigeminal ganglion. We here present, for the first time, the expression of PDE9A, PDE10A, and PDE11A in the trigeminovascular system. The functional implications are yet unknown, but their localisation indicates that they may have a role in the pain pathway of migraine as well as trigeminal neuralgia and trigeminal autonomic cephalalgias.

U2 - 10.1016/j.brainres.2009.05.012

DO - 10.1016/j.brainres.2009.05.012

M3 - Journal article

C2 - 19445908

SN - 0006-8993

VL - 1281

SP - 25

EP - 34

JO - Brain Research

JF - Brain Research

ER -

PDE9A, PDE10A, and PDE11A expression in rat trigeminovascular pain signalling system

Abstract

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