PD-L1 expression by neurons nearby tumors indicates better prognosis in glioblastoma patients

Yawei Liu; Robert Carlsson; Malene Ambjørn; Maruf Hasan; Wiaam Badn; Anna Darabi; Peter Siesjö; Shohreh Navikas

doi:10.1523/JNEUROSCI.5812-12.2013

PD-L1 expression by neurons nearby tumors indicates better prognosis in glioblastoma patients

Yawei Liu, Robert Carlsson, Malene Ambjørn, Maruf Hasan, Wiaam Badn, Anna Darabi, Peter Siesjö, Shohreh Navikas

76 Citations (Scopus)

Abstract

Glioblastoma multiforme (GBM) is the most aggressive form of brain tumor. In general, tumor growth requires disruption of the tissue microenvironment, yet how this affects glioma progression is unknown. We studied program death-ligand (PD-L)1 in neurons and gliomas in tumors from GBM patients and associated the findings with clinical outcome. Remarkably, we found that upregulation of PD-L1 by neurons in tumor-adjacent brain tissue (TABT) associated positively with GBM patient survival, whereas lack of neuronal PD-L1 expression was associated with high PD-L1 in tumors and unfavorable prognosis. To understand the molecular mechanism of PD-L1 signaling in neurons, we investigated PD-L1 function in cerebellar and cortical neurons and its impact on gliomas. We discovered that neuronal PD-L1-induced caspase-dependent apoptosis of glioma cells. Because interferon (IFN)-- induces PD-L1 expression, we studied the functional consequences of neuronal Ifnb gene deletion on PD-L1 signaling and function. Ifnb^-/- neurons lacked PD-L1 and were defective in inducing glioma cell death; this effect was reversed on PD-L1 gene transfection. Ifnb^-/- mice with intracerebral isografts survived poorly. Similar to the observations in GBM patients, better survival in wild-type mice was associated with high neuronal PD-L1 in TABT and downregulation of PD-L1 in tumors, which was defective in Ifnb^-/-mice. Our data indicated that neuronal PD-L1 signaling in brain cells was important forGBMpatient survival. Reciprocal PD-L1 regulation in TABT and tumor tissue could be a prognostic biomarker for GBM. Understanding the complex interactions between tumor and adjacent stromal tissue is important in designing targeted GBM therapies.

Original language	English
Journal	Journal of Neuroscience
Volume	33
Issue number	35
Pages (from-to)	14231-45
Number of pages	15
ISSN	0270-6474
DOIs	https://doi.org/10.1523/JNEUROSCI.5812-12.2013
Publication status	Published - 28 Aug 2013

Keywords

Adult
Aged
Animals
Antigens, CD274
Apoptosis
Brain Neoplasms
Cerebellum
Cerebral Cortex
Female
Gene Deletion
Gene Expression Regulation, Neoplastic
Glioblastoma
Humans
Interferon-beta
Male
Mice
Middle Aged
Neurons
Prognosis
Tumor Markers, Biological

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10.1523/JNEUROSCI.5812-12.2013

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title = "PD-L1 expression by neurons nearby tumors indicates better prognosis in glioblastoma patients",

abstract = "Glioblastoma multiforme (GBM) is the most aggressive form of brain tumor. In general, tumor growth requires disruption of the tissue microenvironment, yet how this affects glioma progression is unknown. We studied program death-ligand (PD-L)1 in neurons and gliomas in tumors from GBM patients and associated the findings with clinical outcome. Remarkably, we found that upregulation of PD-L1 by neurons in tumor-adjacent brain tissue (TABT) associated positively with GBM patient survival, whereas lack of neuronal PD-L1 expression was associated with high PD-L1 in tumors and unfavorable prognosis. To understand the molecular mechanism of PD-L1 signaling in neurons, we investigated PD-L1 function in cerebellar and cortical neurons and its impact on gliomas. We discovered that neuronal PD-L1-induced caspase-dependent apoptosis of glioma cells. Because interferon (IFN)-- induces PD-L1 expression, we studied the functional consequences of neuronal Ifnb gene deletion on PD-L1 signaling and function. Ifnb-/- neurons lacked PD-L1 and were defective in inducing glioma cell death; this effect was reversed on PD-L1 gene transfection. Ifnb-/- mice with intracerebral isografts survived poorly. Similar to the observations in GBM patients, better survival in wild-type mice was associated with high neuronal PD-L1 in TABT and downregulation of PD-L1 in tumors, which was defective in Ifnb-/-mice. Our data indicated that neuronal PD-L1 signaling in brain cells was important forGBMpatient survival. Reciprocal PD-L1 regulation in TABT and tumor tissue could be a prognostic biomarker for GBM. Understanding the complex interactions between tumor and adjacent stromal tissue is important in designing targeted GBM therapies.",

keywords = "Adult, Aged, Animals, Antigens, CD274, Apoptosis, Brain Neoplasms, Cerebellum, Cerebral Cortex, Female, Gene Deletion, Gene Expression Regulation, Neoplastic, Glioblastoma, Humans, Interferon-beta, Male, Mice, Middle Aged, Neurons, Prognosis, Tumor Markers, Biological",

author = "Yawei Liu and Robert Carlsson and Malene Ambj{\o}rn and Maruf Hasan and Wiaam Badn and Anna Darabi and Peter Siesj{\"o} and Shohreh Navikas",

year = "2013",

month = aug,

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doi = "10.1523/JNEUROSCI.5812-12.2013",

language = "English",

volume = "33",

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T1 - PD-L1 expression by neurons nearby tumors indicates better prognosis in glioblastoma patients

AU - Liu, Yawei

AU - Carlsson, Robert

AU - Ambjørn, Malene

AU - Hasan, Maruf

AU - Badn, Wiaam

AU - Darabi, Anna

AU - Siesjö, Peter

AU - Navikas, Shohreh

PY - 2013/8/28

Y1 - 2013/8/28

N2 - Glioblastoma multiforme (GBM) is the most aggressive form of brain tumor. In general, tumor growth requires disruption of the tissue microenvironment, yet how this affects glioma progression is unknown. We studied program death-ligand (PD-L)1 in neurons and gliomas in tumors from GBM patients and associated the findings with clinical outcome. Remarkably, we found that upregulation of PD-L1 by neurons in tumor-adjacent brain tissue (TABT) associated positively with GBM patient survival, whereas lack of neuronal PD-L1 expression was associated with high PD-L1 in tumors and unfavorable prognosis. To understand the molecular mechanism of PD-L1 signaling in neurons, we investigated PD-L1 function in cerebellar and cortical neurons and its impact on gliomas. We discovered that neuronal PD-L1-induced caspase-dependent apoptosis of glioma cells. Because interferon (IFN)-- induces PD-L1 expression, we studied the functional consequences of neuronal Ifnb gene deletion on PD-L1 signaling and function. Ifnb-/- neurons lacked PD-L1 and were defective in inducing glioma cell death; this effect was reversed on PD-L1 gene transfection. Ifnb-/- mice with intracerebral isografts survived poorly. Similar to the observations in GBM patients, better survival in wild-type mice was associated with high neuronal PD-L1 in TABT and downregulation of PD-L1 in tumors, which was defective in Ifnb-/-mice. Our data indicated that neuronal PD-L1 signaling in brain cells was important forGBMpatient survival. Reciprocal PD-L1 regulation in TABT and tumor tissue could be a prognostic biomarker for GBM. Understanding the complex interactions between tumor and adjacent stromal tissue is important in designing targeted GBM therapies.

AB - Glioblastoma multiforme (GBM) is the most aggressive form of brain tumor. In general, tumor growth requires disruption of the tissue microenvironment, yet how this affects glioma progression is unknown. We studied program death-ligand (PD-L)1 in neurons and gliomas in tumors from GBM patients and associated the findings with clinical outcome. Remarkably, we found that upregulation of PD-L1 by neurons in tumor-adjacent brain tissue (TABT) associated positively with GBM patient survival, whereas lack of neuronal PD-L1 expression was associated with high PD-L1 in tumors and unfavorable prognosis. To understand the molecular mechanism of PD-L1 signaling in neurons, we investigated PD-L1 function in cerebellar and cortical neurons and its impact on gliomas. We discovered that neuronal PD-L1-induced caspase-dependent apoptosis of glioma cells. Because interferon (IFN)-- induces PD-L1 expression, we studied the functional consequences of neuronal Ifnb gene deletion on PD-L1 signaling and function. Ifnb-/- neurons lacked PD-L1 and were defective in inducing glioma cell death; this effect was reversed on PD-L1 gene transfection. Ifnb-/- mice with intracerebral isografts survived poorly. Similar to the observations in GBM patients, better survival in wild-type mice was associated with high neuronal PD-L1 in TABT and downregulation of PD-L1 in tumors, which was defective in Ifnb-/-mice. Our data indicated that neuronal PD-L1 signaling in brain cells was important forGBMpatient survival. Reciprocal PD-L1 regulation in TABT and tumor tissue could be a prognostic biomarker for GBM. Understanding the complex interactions between tumor and adjacent stromal tissue is important in designing targeted GBM therapies.

KW - Adult

KW - Aged

KW - Animals

KW - Antigens, CD274

KW - Apoptosis

KW - Brain Neoplasms

KW - Cerebellum

KW - Cerebral Cortex

KW - Female

KW - Gene Deletion

KW - Gene Expression Regulation, Neoplastic

KW - Glioblastoma

KW - Humans

KW - Interferon-beta

KW - Male

KW - Mice

KW - Middle Aged

KW - Neurons

KW - Prognosis

KW - Tumor Markers, Biological

U2 - 10.1523/JNEUROSCI.5812-12.2013

DO - 10.1523/JNEUROSCI.5812-12.2013

M3 - Journal article

C2 - 23986257

SN - 0270-6474

VL - 33

SP - 14231

EP - 14245

JO - Journal of Neuroscience

JF - Journal of Neuroscience

IS - 35

ER -

PD-L1 expression by neurons nearby tumors indicates better prognosis in glioblastoma patients

Abstract

Keywords

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