Pbx Regulates Patterning of the Cerebral Cortex in Progenitors and Postmitotic Neurons

Olga Golonzhka; Alex Nord; Paul L F Tang; Susan Lindtner; Athena R Ypsilanti; Elisabetta Ferretti; Axel Visel; Licia Selleri; John L R Rubenstein

doi:10.1016/j.neuron.2015.10.045

Pbx Regulates Patterning of the Cerebral Cortex in Progenitors and Postmitotic Neurons

Olga Golonzhka, Alex Nord, Paul L F Tang, Susan Lindtner, Athena R Ypsilanti, Elisabetta Ferretti, Axel Visel, Licia Selleri, John L R Rubenstein

The Danish Stem Cell Center

27 Citations (Scopus)

Abstract

We demonstrate using conditional mutagenesis that Pbx1, with and without Pbx2(+/-) sensitization, regulates regional identity and laminar patterning of the developing mouse neocortex in cortical progenitors (Emx1-Cre) and in newly generated neurons (Nex1-Cre). Pbx1/2 mutants have three salient molecular phenotypes of cortical regional and laminar organization: hypoplasia of the frontal cortex, ventral expansion of the dorsomedial cortex, and ventral expansion of Reelin expression in the cortical plate of the frontal cortex, concomitant with an inversion of cortical layering in the rostral cortex. Molecular analyses, including PBX ChIP-seq, provide evidence that PBX promotes frontal cortex identity by repressing genes that promote dorsocaudal fate.

Original language	English
Journal	Neuron
Volume	88
Issue number	6
Pages (from-to)	1192-207
Number of pages	16
ISSN	0896-6273
DOIs	https://doi.org/10.1016/j.neuron.2015.10.045
Publication status	Published - 16 Dec 2015

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10.1016/j.neuron.2015.10.045

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title = "Pbx Regulates Patterning of the Cerebral Cortex in Progenitors and Postmitotic Neurons",

abstract = "We demonstrate using conditional mutagenesis that Pbx1, with and without Pbx2(+/-) sensitization, regulates regional identity and laminar patterning of the developing mouse neocortex in cortical progenitors (Emx1-Cre) and in newly generated neurons (Nex1-Cre). Pbx1/2 mutants have three salient molecular phenotypes of cortical regional and laminar organization: hypoplasia of the frontal cortex, ventral expansion of the dorsomedial cortex, and ventral expansion of Reelin expression in the cortical plate of the frontal cortex, concomitant with an inversion of cortical layering in the rostral cortex. Molecular analyses, including PBX ChIP-seq, provide evidence that PBX promotes frontal cortex identity by repressing genes that promote dorsocaudal fate.",

author = "Olga Golonzhka and Alex Nord and Tang, {Paul L F} and Susan Lindtner and Ypsilanti, {Athena R} and Elisabetta Ferretti and Axel Visel and Licia Selleri and Rubenstein, {John L R}",

year = "2015",

month = dec,

day = "16",

doi = "10.1016/j.neuron.2015.10.045",

language = "English",

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T1 - Pbx Regulates Patterning of the Cerebral Cortex in Progenitors and Postmitotic Neurons

AU - Golonzhka, Olga

AU - Nord, Alex

AU - Tang, Paul L F

AU - Lindtner, Susan

AU - Ypsilanti, Athena R

AU - Ferretti, Elisabetta

AU - Visel, Axel

AU - Selleri, Licia

AU - Rubenstein, John L R

PY - 2015/12/16

Y1 - 2015/12/16

N2 - We demonstrate using conditional mutagenesis that Pbx1, with and without Pbx2(+/-) sensitization, regulates regional identity and laminar patterning of the developing mouse neocortex in cortical progenitors (Emx1-Cre) and in newly generated neurons (Nex1-Cre). Pbx1/2 mutants have three salient molecular phenotypes of cortical regional and laminar organization: hypoplasia of the frontal cortex, ventral expansion of the dorsomedial cortex, and ventral expansion of Reelin expression in the cortical plate of the frontal cortex, concomitant with an inversion of cortical layering in the rostral cortex. Molecular analyses, including PBX ChIP-seq, provide evidence that PBX promotes frontal cortex identity by repressing genes that promote dorsocaudal fate.

AB - We demonstrate using conditional mutagenesis that Pbx1, with and without Pbx2(+/-) sensitization, regulates regional identity and laminar patterning of the developing mouse neocortex in cortical progenitors (Emx1-Cre) and in newly generated neurons (Nex1-Cre). Pbx1/2 mutants have three salient molecular phenotypes of cortical regional and laminar organization: hypoplasia of the frontal cortex, ventral expansion of the dorsomedial cortex, and ventral expansion of Reelin expression in the cortical plate of the frontal cortex, concomitant with an inversion of cortical layering in the rostral cortex. Molecular analyses, including PBX ChIP-seq, provide evidence that PBX promotes frontal cortex identity by repressing genes that promote dorsocaudal fate.

U2 - 10.1016/j.neuron.2015.10.045

DO - 10.1016/j.neuron.2015.10.045

M3 - Journal article

C2 - 26671461

SN - 0896-6273

VL - 88

SP - 1192

EP - 1207

JO - Neuron

JF - Neuron

IS - 6

ER -

Pbx Regulates Patterning of the Cerebral Cortex in Progenitors and Postmitotic Neurons

Abstract

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