Pax4 represses pancreatic glucagon gene expression

H V Petersen; Mette Christine Jørgensen; F G Andersen; J Jensen; T F-Nielsen; R Jørgensen; O D Madsen; Palle Serup

doi:10.1006/mcbr.2000.0220

Pax4 represses pancreatic glucagon gene expression

H V Petersen, Mette Christine Jørgensen, F G Andersen, J Jensen, T F-Nielsen, R Jørgensen, O D Madsen, Palle Serup

31 Citations (Scopus)

Abstract

The paired box and homeodomain containing transcription factors Pax4 and Pax6 are known to be essential for development of the pancreatic endocrine cells. In this report we demonstrate that stable expression of Pax4 in a rat glucagon-producing cell line inhibits the endogenously expressed glucagon gene completely. Furthermore, Pax4 represses Pax6 independent transcription of the insulin promoter, suggesting that Pax4 can actively repress transcription in addition to acting by competition with the transcriptional activator Pax6.

Original language	English
Journal	Molecular Cell Biology Research Communications
Volume	3
Issue number	4
Pages (from-to)	249-54
Number of pages	6
ISSN	1522-4724
DOIs	https://doi.org/10.1006/mcbr.2000.0220
Publication status	Published - Apr 2000
Externally published	Yes

Keywords

Animals
Cell Line
DNA-Binding Proteins
Eye Proteins
Fluorescent Antibody Technique
Gene Expression Regulation
Glucagon
Homeodomain Proteins
In Situ Hybridization
Insulin
Islets of Langerhans
Mice
Paired Box Transcription Factors
Promoter Regions, Genetic
RNA, Messenger
Rats
Repressor Proteins
Reverse Transcriptase Polymerase Chain Reaction
Transcription Factors
Transfection
Tumor Cells, Cultured

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10.1006/mcbr.2000.0220

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title = "Pax4 represses pancreatic glucagon gene expression",

abstract = "The paired box and homeodomain containing transcription factors Pax4 and Pax6 are known to be essential for development of the pancreatic endocrine cells. In this report we demonstrate that stable expression of Pax4 in a rat glucagon-producing cell line inhibits the endogenously expressed glucagon gene completely. Furthermore, Pax4 represses Pax6 independent transcription of the insulin promoter, suggesting that Pax4 can actively repress transcription in addition to acting by competition with the transcriptional activator Pax6.",

keywords = "Animals, Cell Line, DNA-Binding Proteins, Eye Proteins, Fluorescent Antibody Technique, Gene Expression Regulation, Glucagon, Homeodomain Proteins, In Situ Hybridization, Insulin, Islets of Langerhans, Mice, Paired Box Transcription Factors, Promoter Regions, Genetic, RNA, Messenger, Rats, Repressor Proteins, Reverse Transcriptase Polymerase Chain Reaction, Transcription Factors, Transfection, Tumor Cells, Cultured",

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year = "2000",

month = apr,

doi = "10.1006/mcbr.2000.0220",

language = "English",

volume = "3",

pages = "249--54",

journal = "Molecular Cell Biology Research Communications",

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TY - JOUR

T1 - Pax4 represses pancreatic glucagon gene expression

AU - Petersen, H V

AU - Jørgensen, Mette Christine

AU - Andersen, F G

AU - Jensen, J

AU - F-Nielsen, T

AU - Jørgensen, R

AU - Madsen, O D

AU - Serup, Palle

PY - 2000/4

Y1 - 2000/4

N2 - The paired box and homeodomain containing transcription factors Pax4 and Pax6 are known to be essential for development of the pancreatic endocrine cells. In this report we demonstrate that stable expression of Pax4 in a rat glucagon-producing cell line inhibits the endogenously expressed glucagon gene completely. Furthermore, Pax4 represses Pax6 independent transcription of the insulin promoter, suggesting that Pax4 can actively repress transcription in addition to acting by competition with the transcriptional activator Pax6.

AB - The paired box and homeodomain containing transcription factors Pax4 and Pax6 are known to be essential for development of the pancreatic endocrine cells. In this report we demonstrate that stable expression of Pax4 in a rat glucagon-producing cell line inhibits the endogenously expressed glucagon gene completely. Furthermore, Pax4 represses Pax6 independent transcription of the insulin promoter, suggesting that Pax4 can actively repress transcription in addition to acting by competition with the transcriptional activator Pax6.

KW - Animals

KW - Cell Line

KW - DNA-Binding Proteins

KW - Eye Proteins

KW - Fluorescent Antibody Technique

KW - Gene Expression Regulation

KW - Glucagon

KW - Homeodomain Proteins

KW - In Situ Hybridization

KW - Insulin

KW - Islets of Langerhans

KW - Mice

KW - Paired Box Transcription Factors

KW - Promoter Regions, Genetic

KW - RNA, Messenger

KW - Rats

KW - Repressor Proteins

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Transcription Factors

KW - Transfection

KW - Tumor Cells, Cultured

U2 - 10.1006/mcbr.2000.0220

DO - 10.1006/mcbr.2000.0220

M3 - Journal article

C2 - 10891400

SN - 1522-4724

VL - 3

SP - 249

EP - 254

JO - Molecular Cell Biology Research Communications

JF - Molecular Cell Biology Research Communications

IS - 4

ER -

Pax4 represses pancreatic glucagon gene expression

Abstract

Keywords

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