Pattern of distribution of blood group antigens on human epidermal cells during maturation

Erik Dabelsteen; Karsten Buschard; Sen-Itiroh Hakomori

Pattern of distribution of blood group antigens on human epidermal cells during maturation

Erik Dabelsteen, Karsten Buschard, Sen-Itiroh Hakomori

Section 03 - Radiology

46 Citations (Scopus)

Abstract

The distribution in human epidermis of A, B, and H blood group antigens and of a precursor carbohydrate chain, N-acetyl-lactosamine, was examined using immunofluorescence staining techniques. The material included tissue from 10 blood group A, 4 blood group B, and 9 blood group O persons. Murine monoclonal antibodies were used to identify H antigen (type 2 chain) and N-acetyl-lactosamine. Human antisera were used to identify A and B antigens. In all groups N-acetyl-lactosamine and H antigen were found on the cell membranes of the spinous cell layer. N-acetyl-lactosamine was present mainly on the lower spinous cells whereas H antigen was seen predominantly on upper spinous cells or on the granular cells. Epithelia from blood group A or B persons demonstrated A or B antigens, respectively, but only if the tissue sections were trypsinized before staining. In such cases A or B antigens were found on the cell membranes in the granular cell layer.

Original language	English
Journal	Journal of Investigative Dermatology
Volume	82
Pages (from-to)	13-7
ISSN	0022-202X
Publication status	Published - 1984

Cite this

@article{467060527a9c464ca13e8957f56c4c21,

title = "Pattern of distribution of blood group antigens on human epidermal cells during maturation",

abstract = "The distribution in human epidermis of A, B, and H blood group antigens and of a precursor carbohydrate chain, N-acetyl-lactosamine, was examined using immunofluorescence staining techniques. The material included tissue from 10 blood group A, 4 blood group B, and 9 blood group O persons. Murine monoclonal antibodies were used to identify H antigen (type 2 chain) and N-acetyl-lactosamine. Human antisera were used to identify A and B antigens. In all groups N-acetyl-lactosamine and H antigen were found on the cell membranes of the spinous cell layer. N-acetyl-lactosamine was present mainly on the lower spinous cells whereas H antigen was seen predominantly on upper spinous cells or on the granular cells. Epithelia from blood group A or B persons demonstrated A or B antigens, respectively, but only if the tissue sections were trypsinized before staining. In such cases A or B antigens were found on the cell membranes in the granular cell layer.",

author = "Erik Dabelsteen and Karsten Buschard and Sen-Itiroh Hakomori",

year = "1984",

language = "English",

volume = "82",

pages = "13--7",

journal = "Journal of Investigative Dermatology",

issn = "0022-202X",

publisher = "nature publishing group",

}

TY - JOUR

T1 - Pattern of distribution of blood group antigens on human epidermal cells during maturation

AU - Dabelsteen, Erik

AU - Buschard, Karsten

AU - Hakomori, Sen-Itiroh

PY - 1984

Y1 - 1984

N2 - The distribution in human epidermis of A, B, and H blood group antigens and of a precursor carbohydrate chain, N-acetyl-lactosamine, was examined using immunofluorescence staining techniques. The material included tissue from 10 blood group A, 4 blood group B, and 9 blood group O persons. Murine monoclonal antibodies were used to identify H antigen (type 2 chain) and N-acetyl-lactosamine. Human antisera were used to identify A and B antigens. In all groups N-acetyl-lactosamine and H antigen were found on the cell membranes of the spinous cell layer. N-acetyl-lactosamine was present mainly on the lower spinous cells whereas H antigen was seen predominantly on upper spinous cells or on the granular cells. Epithelia from blood group A or B persons demonstrated A or B antigens, respectively, but only if the tissue sections were trypsinized before staining. In such cases A or B antigens were found on the cell membranes in the granular cell layer.

AB - The distribution in human epidermis of A, B, and H blood group antigens and of a precursor carbohydrate chain, N-acetyl-lactosamine, was examined using immunofluorescence staining techniques. The material included tissue from 10 blood group A, 4 blood group B, and 9 blood group O persons. Murine monoclonal antibodies were used to identify H antigen (type 2 chain) and N-acetyl-lactosamine. Human antisera were used to identify A and B antigens. In all groups N-acetyl-lactosamine and H antigen were found on the cell membranes of the spinous cell layer. N-acetyl-lactosamine was present mainly on the lower spinous cells whereas H antigen was seen predominantly on upper spinous cells or on the granular cells. Epithelia from blood group A or B persons demonstrated A or B antigens, respectively, but only if the tissue sections were trypsinized before staining. In such cases A or B antigens were found on the cell membranes in the granular cell layer.

M3 - Journal article

SN - 0022-202X

VL - 82

SP - 13

EP - 17

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

ER -