Abstract
Background: This paper reports development of a new approach towards analytical liquid-liquid-liquid membrane extraction termed parallel artificial liquid membrane extraction. A donor plate and acceptor plate create a sandwich, in which each sample (human plasma) and acceptor solution is separated by an artificial liquid membrane. Parallel artificial liquid membrane extraction is a modification of hollow-fiber liquid-phase microextraction, where the hollow fibers are replaced by flat membranes in a 96-well plate format. Results: Four basic drugs (pethidine, nortriptyline, methadone and haloperidol) were extracted from human plasma in 30 min, followed by analysis with LC-MS/MS. Extraction recoveries for the model analytes were in the range of 34-74% from human plasma. LOQs were in the range of 0.01-0.35 ng/ml, linearity above 0.9955 for all drugs and with RSD values below 12%. Conclusion: Liquid-liquid-liquid membrane extraction was successfully performed in a slightly modified commercially available 96-well plate format.
| Original language | English |
|---|---|
| Journal | Bioanalysis |
| Volume | 5 |
| Issue number | 11 |
| Pages (from-to) | 1377-85 |
| Number of pages | 9 |
| ISSN | 1757-6180 |
| DOIs | |
| Publication status | Published - Jun 2013 |
Keywords
- Chromatography, Liquid
- Equipment Design
- Humans
- Limit of Detection
- Liquid Phase Microextraction
- Membranes, Artificial
- Pharmaceutical Preparations
- Tandem Mass Spectrometry