Oxygen restriction increases the infective potential of Listeria monocytogenes in vitro in Caco-2 cells and in vivo in guinea pigs

TY - JOUR

T1 - Oxygen restriction increases the infective potential of Listeria monocytogenes in vitro in Caco-2 cells and in vivo in guinea pigs

AU - Andersen, Jens Bo

AU - Roldgaard, Bent

AU - Christensen, Bjarke Bak

AU - Licht, Tine Rask

PY - 2007

Y1 - 2007

N2 - Listeria monocytogenes has been implicated in several food borne outbreaks as well as sporadic cases of disease. Increased understanding of the biology of this organism is important in the prevention of food borne listeriosis. The infectivity of Listeria monocytogenes ScottA, cultivated with and without oxygen restriction, was compared in vitro and in vivo. Fluorescent protein labels were applied to allow certain identification of Listeria cells from untagged bacteria in in vivo samples, and to distinguish between cells grown under different conditions in mixed infection experiments.

AB - Listeria monocytogenes has been implicated in several food borne outbreaks as well as sporadic cases of disease. Increased understanding of the biology of this organism is important in the prevention of food borne listeriosis. The infectivity of Listeria monocytogenes ScottA, cultivated with and without oxygen restriction, was compared in vitro and in vivo. Fluorescent protein labels were applied to allow certain identification of Listeria cells from untagged bacteria in in vivo samples, and to distinguish between cells grown under different conditions in mixed infection experiments.

KW - Animals

KW - Bacteriological Techniques

KW - Caco-2 Cells

KW - Guinea Pigs

KW - Humans

KW - Listeria monocytogenes

KW - Listeriosis

KW - Oxygen

U2 - 10.1186/1471-2180-7-55

DO - 10.1186/1471-2180-7-55

M3 - Journal article

C2 - 17570840

SN - 1471-2180

VL - 7

SP - 55

JO - BMC Microbiology

JF - BMC Microbiology

ER -