Abstract
We present a new fiber-optic oxygen microsensor based on dynamic luminescence quenching which was recently developed for measuring oxygen at high spatial resolution in aquatic sediments and biofilms. Micro-optrodes with a typical tip diameter of 20 to 50 μm were fabricated. The fabrication procedure is simple and guarantees a high reproducibility of the calibration curves. The micro-optrodes were characterized with respect to dynamic range, response time, storage, longterm stability, interferences, temperature dependence, photostability, and mechanical stability. A special LED based luminescence intensity measuring instrument was developed. It is battery operated and can be used for field measurements. The micro-optrodes were used to measure oxygen gradients in marine sediments. Comparative measurements were performed with oxygen microelectrodes. The first measurements have shown that oxygen micro-optrodes present a true alternative to existing electrochemical microsensors. Nevertheless, it is obvious, that the measurement of luminescence intensity of the indicator limits their practical application. Therefore a new setup was developed to make oxygen measurements with the luminescence lifetime as parameter.
| Original language | English |
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| Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
| Number of pages | 12 |
| Volume | 2508 |
| Publication date | 1 Dec 1995 |
| Pages | 375-386 |
| ISBN (Print) | 0819418668, 9780819418661 |
| Publication status | Published - 1 Dec 1995 |
| Event | Chemical, Biochemical, and Environmental Fiber Sensors VII - Munich, Ger Duration: 19 Jun 1995 → 20 Jun 1995 |
Conference
| Conference | Chemical, Biochemical, and Environmental Fiber Sensors VII |
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| City | Munich, Ger |
| Period | 19/06/1995 → 20/06/1995 |
| Sponsor | SPIE - Int Soc for Opt Engineering, Bellingham, WA USA |
