Oxygen-dependent vasomotor responses are conducted upstream in the mouse cremaster microcirculation

Mads Riemann; Amrit Rai; Anh Thuc Ngo; Morten Hanefeld Dziegiel; Niels-Henrik Holstein-Rathlou; Christian Torp-Pedersen; Mads Achen Riemann; Amrit Rai; Anh Thuc Ngo; Morten Hanefeld Dziegiel; Niels-Henrik Holstein-Rathlou; Christian Torp-Pedersen

doi:10.1159/000318777

Oxygen-dependent vasomotor responses are conducted upstream in the mouse cremaster microcirculation

Mads Riemann, Amrit Rai, Anh Thuc Ngo, Morten Hanefeld Dziegiel, Niels-Henrik Holstein-Rathlou, Christian Torp-Pedersen, Mads Achen Riemann, Amrit Rai, Anh Thuc Ngo, Morten Hanefeld Dziegiel, Niels-Henrik Holstein-Rathlou, Christian Torp-Pedersen

11 Citations (Scopus)

Abstract

Background: A new method was evaluated where local changes in oxygen tension were induced in a tissue while being studied under a microscope in vivo. We tested whether hypoxic vasodilation and hyperoxic vasoconstriction in arterioles in striated muscle are being propagated upstream, and whether the endothelium and smooth muscle cell layers are necessary components in the signaling pathway. Methods: The study was performed in mouse cremaster muscle superfused with Krebs buffer. A section of the capillary bed was then superfused with human red blood cell suspension equilibrated with either 95% nitrogen or 95% oxygen, and 5% carbon dioxide. Results: The superfusions caused a 12.9 ± 2.4% (p < 0.01) dilation and a 12.3 ± 2.7% (p < 0.01) constriction of the supplying non-exposed arteriole. Vasomotor responses could be detected 1 mm upstream of the stimulation site. The responses to hypoxia and hyperoxia were not affected by inhibition of nitric oxide (NO) synthases by L-NAME. Damage to the wall of an intervening segment of the arteriole abolished upstream changes. Conclusions: The method is capable of changing the oxygen tension locally in a membranous tissue and elicits NO-independent local and upstream vasomotor responses. Upstream responses were transmitted by a conducted vascular response.

Original language	English
Journal	Journal of Vascular Research
Volume	48
Issue number	1
Pages (from-to)	79-89
Number of pages	11
ISSN	1018-1172
DOIs	https://doi.org/10.1159/000318777 https://doi.org/10.1159/000318777
Publication status	Published - Dec 2010

Access to Document

Cite this

Riemann, M., Rai, A., Ngo, A. T., Dziegiel, M. H., Holstein-Rathlou, N.-H., Torp-Pedersen, C., Riemann, M. A., Rai, A., Ngo, A. T., Dziegiel, M. H., Holstein-Rathlou, N.-H., & Torp-Pedersen, C. (2010). Oxygen-dependent vasomotor responses are conducted upstream in the mouse cremaster microcirculation. Journal of Vascular Research, 48(1), 79-89. https://doi.org/10.1159/000318777, https://doi.org/10.1159/000318777

Riemann, M, Rai, A, Ngo, AT, Dziegiel, MH , Holstein-Rathlou, N-H , Torp-Pedersen, C, Riemann, MA, Rai, A, Ngo, AT, Dziegiel, MH, Holstein-Rathlou, N-H & Torp-Pedersen, C 2010, 'Oxygen-dependent vasomotor responses are conducted upstream in the mouse cremaster microcirculation', Journal of Vascular Research, vol. 48, no. 1, pp. 79-89. https://doi.org/10.1159/000318777, https://doi.org/10.1159/000318777

@article{24686720a9de11df928f000ea68e967b,

title = "Oxygen-dependent vasomotor responses are conducted upstream in the mouse cremaster microcirculation",

abstract = "Background: A new method was evaluated where local changes in oxygen tension were induced in a tissue while being studied under a microscope in vivo. We tested whether hypoxic vasodilation and hyperoxic vasoconstriction in arterioles in striated muscle are being propagated upstream, and whether the endothelium and smooth muscle cell layers are necessary components in the signaling pathway. Methods: The study was performed in mouse cremaster muscle superfused with Krebs buffer. A section of the capillary bed was then superfused with human red blood cell suspension equilibrated with either 95% nitrogen or 95% oxygen, and 5% carbon dioxide. Results: The superfusions caused a 12.9 ± 2.4% (p < 0.01) dilation and a 12.3 ± 2.7% (p < 0.01) constriction of the supplying non-exposed arteriole. Vasomotor responses could be detected 1 mm upstream of the stimulation site. The responses to hypoxia and hyperoxia were not affected by inhibition of nitric oxide (NO) synthases by L-NAME. Damage to the wall of an intervening segment of the arteriole abolished upstream changes. Conclusions: The method is capable of changing the oxygen tension locally in a membranous tissue and elicits NO-independent local and upstream vasomotor responses. Upstream responses were transmitted by a conducted vascular response.",

author = "Mads Riemann and Amrit Rai and Ngo, {Anh Thuc} and Dziegiel, {Morten Hanefeld} and Niels-Henrik Holstein-Rathlou and Christian Torp-Pedersen and Riemann, {Mads Achen} and Amrit Rai and Ngo, {Anh Thuc} and Dziegiel, {Morten Hanefeld} and Niels-Henrik Holstein-Rathlou and Christian Torp-Pedersen",

note = "Copyright {\textcopyright} 2010 S. Karger AG, Basel.",

year = "2010",

month = dec,

doi = "10.1159/000318777",

language = "English",

volume = "48",

pages = "79--89",

journal = "Journal of Vascular Research",

issn = "1018-1172",

publisher = "S Karger AG",

number = "1",

}

TY - JOUR

T1 - Oxygen-dependent vasomotor responses are conducted upstream in the mouse cremaster microcirculation

AU - Riemann, Mads

AU - Rai, Amrit

AU - Ngo, Anh Thuc

AU - Dziegiel, Morten Hanefeld

AU - Holstein-Rathlou, Niels-Henrik

AU - Torp-Pedersen, Christian

AU - Riemann, Mads Achen

AU - Rai, Amrit

AU - Ngo, Anh Thuc

AU - Dziegiel, Morten Hanefeld

AU - Holstein-Rathlou, Niels-Henrik

AU - Torp-Pedersen, Christian

PY - 2010/12

Y1 - 2010/12

N2 - Background: A new method was evaluated where local changes in oxygen tension were induced in a tissue while being studied under a microscope in vivo. We tested whether hypoxic vasodilation and hyperoxic vasoconstriction in arterioles in striated muscle are being propagated upstream, and whether the endothelium and smooth muscle cell layers are necessary components in the signaling pathway. Methods: The study was performed in mouse cremaster muscle superfused with Krebs buffer. A section of the capillary bed was then superfused with human red blood cell suspension equilibrated with either 95% nitrogen or 95% oxygen, and 5% carbon dioxide. Results: The superfusions caused a 12.9 ± 2.4% (p < 0.01) dilation and a 12.3 ± 2.7% (p < 0.01) constriction of the supplying non-exposed arteriole. Vasomotor responses could be detected 1 mm upstream of the stimulation site. The responses to hypoxia and hyperoxia were not affected by inhibition of nitric oxide (NO) synthases by L-NAME. Damage to the wall of an intervening segment of the arteriole abolished upstream changes. Conclusions: The method is capable of changing the oxygen tension locally in a membranous tissue and elicits NO-independent local and upstream vasomotor responses. Upstream responses were transmitted by a conducted vascular response.

AB - Background: A new method was evaluated where local changes in oxygen tension were induced in a tissue while being studied under a microscope in vivo. We tested whether hypoxic vasodilation and hyperoxic vasoconstriction in arterioles in striated muscle are being propagated upstream, and whether the endothelium and smooth muscle cell layers are necessary components in the signaling pathway. Methods: The study was performed in mouse cremaster muscle superfused with Krebs buffer. A section of the capillary bed was then superfused with human red blood cell suspension equilibrated with either 95% nitrogen or 95% oxygen, and 5% carbon dioxide. Results: The superfusions caused a 12.9 ± 2.4% (p < 0.01) dilation and a 12.3 ± 2.7% (p < 0.01) constriction of the supplying non-exposed arteriole. Vasomotor responses could be detected 1 mm upstream of the stimulation site. The responses to hypoxia and hyperoxia were not affected by inhibition of nitric oxide (NO) synthases by L-NAME. Damage to the wall of an intervening segment of the arteriole abolished upstream changes. Conclusions: The method is capable of changing the oxygen tension locally in a membranous tissue and elicits NO-independent local and upstream vasomotor responses. Upstream responses were transmitted by a conducted vascular response.

U2 - 10.1159/000318777

DO - 10.1159/000318777

M3 - Journal article

C2 - 20639650

SN - 1018-1172

VL - 48

SP - 79

EP - 89

JO - Journal of Vascular Research

JF - Journal of Vascular Research

IS - 1

ER -

Oxygen-dependent vasomotor responses are conducted upstream in the mouse cremaster microcirculation

Abstract

Access to Document

Fingerprint

Cite this