Outdoor air pollution exposures and micronuclei frequencies in lymphocytes from pregnant women and newborns in Crete, Greece (Rhea cohort)

Cristina O'Callaghan-Gordo; Eleni Fthenou; Marie Pedersen; Ana Espinosa; Leda Chatzi; Rob Beelen; Georgia Chalkiadaki; Ilse Decordier; Gerard Hoek; Domenico Franco Merlo; Mark Nieuwenhuijsen; Theano Roumeliotaki; Marina Vafeiadi; Kim Vande Loock; Jos Kleinjans; Euripides Stephanou; Micheline Kirsch-Volders; Manolis Kogevinas

doi:10.1016/j.envres.2015.10.011

Outdoor air pollution exposures and micronuclei frequencies in lymphocytes from pregnant women and newborns in Crete, Greece (Rhea cohort)

Cristina O'Callaghan-Gordo, Eleni Fthenou, Marie Pedersen, Ana Espinosa, Leda Chatzi, Rob Beelen, Georgia Chalkiadaki, Ilse Decordier, Gerard Hoek, Domenico Franco Merlo, Mark Nieuwenhuijsen, Theano Roumeliotaki, Marina Vafeiadi, Kim Vande Loock, Jos Kleinjans, Euripides Stephanou, Micheline Kirsch-Volders, Manolis Kogevinas

18 Citations (Scopus)

Abstract

BACKGROUND: Micronuclei (MN) are biomarkers of early genetic effects that have been used to investigate the association between environmental exposures and cancer. However, few studies have examined the association between environmental exposures during pregnancy and MN in mothers and newborns.

OBJECTIVES: We examined MN frequency in maternal blood and in cord blood, in relation to maternal air pollution exposure, and the potential interaction with maternal vitamin C intake and maternal smoking.

METHODS: We used the cytokinesis-block micronucleus assay to assess MN frequency per 1000 bi-nucleated T-lymphocytes from 181 mothers and 183 newborns born in 2007-2008 in Heraklion (Crete, Greece). The ESCAPE land-use regression methods were used to estimate annual mean exposure to outdoor air pollution [particulate matter (PM), black carbon, nitrogen dioxide (NO2) and nitrogen oxides (NOx)] at maternal home addresses. Food frequency questionnaires were used to estimate maternal dietary vitamin C intake during pregnancy. Smoking habits were self-reported using questionnaires which were checked by measuring maternal urinary cotinine levels.

RESULTS: Exposure to PM2.5 was associated with increased MN frequencies in pregnant women [rate ratio [RR (95%CI)] per 5µg/m(3)=1.53 (1.02, 2.29)]. This increase was considerably higher among women who did not fulfill the recommended vitamin C dietary allowances [RR=9.35 (2.77, 31.61); n=20]. Exposure to PM2.5-10, PM10, NO2 and NOx were also associated with a higher incidence of MN frequencies in smoker women (n=56). No associations were found for newborns.

CONCLUSIONS: We found an association between air pollution, particularly PM2.5, and MN frequency in mothers but not in newborns. This association was more pronounced among women with a lower dietary intake of vitamin C during pregnancy and among women who smoked during pregnancy. While results are clear in mothers, the association between maternal carcinogenic exposures during pregnancy and biomarkers of early biologic effect in the newborn remains poorly understood.

Original language	English
Journal	Environmental Research
Volume	143
Issue number	Part A
Pages (from-to)	170-176
Number of pages	7
ISSN	0013-9351
DOIs	https://doi.org/10.1016/j.envres.2015.10.011
Publication status	Published - 1 Nov 2015
Externally published	Yes

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O'Callaghan-Gordo, C., Fthenou, E., Pedersen, M., Espinosa, A., Chatzi, L., Beelen, R., Chalkiadaki, G., Decordier, I., Hoek, G., Merlo, D. F., Nieuwenhuijsen, M., Roumeliotaki, T., Vafeiadi, M., Loock, K. V., Kleinjans, J., Stephanou, E., Kirsch-Volders, M., & Kogevinas, M. (2015). Outdoor air pollution exposures and micronuclei frequencies in lymphocytes from pregnant women and newborns in Crete, Greece (Rhea cohort). Environmental Research, 143(Part A), 170-176. https://doi.org/10.1016/j.envres.2015.10.011

Outdoor air pollution exposures and micronuclei frequencies in lymphocytes from pregnant women and newborns in Crete, Greece (Rhea cohort). / O'Callaghan-Gordo, Cristina; Fthenou, Eleni; Pedersen, Marie et al.

In: Environmental Research, Vol. 143, No. Part A, 01.11.2015, p. 170-176.

Research output: Contribution to journal › Journal article › Research › peer-review

O'Callaghan-Gordo, C, Fthenou, E, Pedersen, M, Espinosa, A, Chatzi, L, Beelen, R, Chalkiadaki, G, Decordier, I, Hoek, G, Merlo, DF, Nieuwenhuijsen, M, Roumeliotaki, T, Vafeiadi, M, Loock, KV, Kleinjans, J, Stephanou, E, Kirsch-Volders, M & Kogevinas, M 2015, 'Outdoor air pollution exposures and micronuclei frequencies in lymphocytes from pregnant women and newborns in Crete, Greece (Rhea cohort)', Environmental Research, vol. 143, no. Part A, pp. 170-176. https://doi.org/10.1016/j.envres.2015.10.011

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title = "Outdoor air pollution exposures and micronuclei frequencies in lymphocytes from pregnant women and newborns in Crete, Greece (Rhea cohort)",

abstract = "BACKGROUND: Micronuclei (MN) are biomarkers of early genetic effects that have been used to investigate the association between environmental exposures and cancer. However, few studies have examined the association between environmental exposures during pregnancy and MN in mothers and newborns.OBJECTIVES: We examined MN frequency in maternal blood and in cord blood, in relation to maternal air pollution exposure, and the potential interaction with maternal vitamin C intake and maternal smoking.METHODS: We used the cytokinesis-block micronucleus assay to assess MN frequency per 1000 bi-nucleated T-lymphocytes from 181 mothers and 183 newborns born in 2007-2008 in Heraklion (Crete, Greece). The ESCAPE land-use regression methods were used to estimate annual mean exposure to outdoor air pollution [particulate matter (PM), black carbon, nitrogen dioxide (NO2) and nitrogen oxides (NOx)] at maternal home addresses. Food frequency questionnaires were used to estimate maternal dietary vitamin C intake during pregnancy. Smoking habits were self-reported using questionnaires which were checked by measuring maternal urinary cotinine levels.RESULTS: Exposure to PM2.5 was associated with increased MN frequencies in pregnant women [rate ratio [RR (95%CI)] per 5µg/m(3)=1.53 (1.02, 2.29)]. This increase was considerably higher among women who did not fulfill the recommended vitamin C dietary allowances [RR=9.35 (2.77, 31.61); n=20]. Exposure to PM2.5-10, PM10, NO2 and NOx were also associated with a higher incidence of MN frequencies in smoker women (n=56). No associations were found for newborns.CONCLUSIONS: We found an association between air pollution, particularly PM2.5, and MN frequency in mothers but not in newborns. This association was more pronounced among women with a lower dietary intake of vitamin C during pregnancy and among women who smoked during pregnancy. While results are clear in mothers, the association between maternal carcinogenic exposures during pregnancy and biomarkers of early biologic effect in the newborn remains poorly understood.",

author = "Cristina O'Callaghan-Gordo and Eleni Fthenou and Marie Pedersen and Ana Espinosa and Leda Chatzi and Rob Beelen and Georgia Chalkiadaki and Ilse Decordier and Gerard Hoek and Merlo, {Domenico Franco} and Mark Nieuwenhuijsen and Theano Roumeliotaki and Marina Vafeiadi and Loock, {Kim Vande} and Jos Kleinjans and Euripides Stephanou and Micheline Kirsch-Volders and Manolis Kogevinas",

year = "2015",

month = nov,

day = "1",

doi = "10.1016/j.envres.2015.10.011",

language = "English",

volume = "143",

pages = "170--176",

journal = "Environmental Research",

issn = "0013-9351",

publisher = "Academic Press",

number = "Part A",

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TY - JOUR

T1 - Outdoor air pollution exposures and micronuclei frequencies in lymphocytes from pregnant women and newborns in Crete, Greece (Rhea cohort)

AU - O'Callaghan-Gordo, Cristina

AU - Fthenou, Eleni

AU - Pedersen, Marie

AU - Espinosa, Ana

AU - Chatzi, Leda

AU - Beelen, Rob

AU - Chalkiadaki, Georgia

AU - Decordier, Ilse

AU - Hoek, Gerard

AU - Merlo, Domenico Franco

AU - Nieuwenhuijsen, Mark

AU - Roumeliotaki, Theano

AU - Vafeiadi, Marina

AU - Loock, Kim Vande

AU - Kleinjans, Jos

AU - Stephanou, Euripides

AU - Kirsch-Volders, Micheline

AU - Kogevinas, Manolis

PY - 2015/11/1

Y1 - 2015/11/1

N2 - BACKGROUND: Micronuclei (MN) are biomarkers of early genetic effects that have been used to investigate the association between environmental exposures and cancer. However, few studies have examined the association between environmental exposures during pregnancy and MN in mothers and newborns.OBJECTIVES: We examined MN frequency in maternal blood and in cord blood, in relation to maternal air pollution exposure, and the potential interaction with maternal vitamin C intake and maternal smoking.METHODS: We used the cytokinesis-block micronucleus assay to assess MN frequency per 1000 bi-nucleated T-lymphocytes from 181 mothers and 183 newborns born in 2007-2008 in Heraklion (Crete, Greece). The ESCAPE land-use regression methods were used to estimate annual mean exposure to outdoor air pollution [particulate matter (PM), black carbon, nitrogen dioxide (NO2) and nitrogen oxides (NOx)] at maternal home addresses. Food frequency questionnaires were used to estimate maternal dietary vitamin C intake during pregnancy. Smoking habits were self-reported using questionnaires which were checked by measuring maternal urinary cotinine levels.RESULTS: Exposure to PM2.5 was associated with increased MN frequencies in pregnant women [rate ratio [RR (95%CI)] per 5µg/m(3)=1.53 (1.02, 2.29)]. This increase was considerably higher among women who did not fulfill the recommended vitamin C dietary allowances [RR=9.35 (2.77, 31.61); n=20]. Exposure to PM2.5-10, PM10, NO2 and NOx were also associated with a higher incidence of MN frequencies in smoker women (n=56). No associations were found for newborns.CONCLUSIONS: We found an association between air pollution, particularly PM2.5, and MN frequency in mothers but not in newborns. This association was more pronounced among women with a lower dietary intake of vitamin C during pregnancy and among women who smoked during pregnancy. While results are clear in mothers, the association between maternal carcinogenic exposures during pregnancy and biomarkers of early biologic effect in the newborn remains poorly understood.

AB - BACKGROUND: Micronuclei (MN) are biomarkers of early genetic effects that have been used to investigate the association between environmental exposures and cancer. However, few studies have examined the association between environmental exposures during pregnancy and MN in mothers and newborns.OBJECTIVES: We examined MN frequency in maternal blood and in cord blood, in relation to maternal air pollution exposure, and the potential interaction with maternal vitamin C intake and maternal smoking.METHODS: We used the cytokinesis-block micronucleus assay to assess MN frequency per 1000 bi-nucleated T-lymphocytes from 181 mothers and 183 newborns born in 2007-2008 in Heraklion (Crete, Greece). The ESCAPE land-use regression methods were used to estimate annual mean exposure to outdoor air pollution [particulate matter (PM), black carbon, nitrogen dioxide (NO2) and nitrogen oxides (NOx)] at maternal home addresses. Food frequency questionnaires were used to estimate maternal dietary vitamin C intake during pregnancy. Smoking habits were self-reported using questionnaires which were checked by measuring maternal urinary cotinine levels.RESULTS: Exposure to PM2.5 was associated with increased MN frequencies in pregnant women [rate ratio [RR (95%CI)] per 5µg/m(3)=1.53 (1.02, 2.29)]. This increase was considerably higher among women who did not fulfill the recommended vitamin C dietary allowances [RR=9.35 (2.77, 31.61); n=20]. Exposure to PM2.5-10, PM10, NO2 and NOx were also associated with a higher incidence of MN frequencies in smoker women (n=56). No associations were found for newborns.CONCLUSIONS: We found an association between air pollution, particularly PM2.5, and MN frequency in mothers but not in newborns. This association was more pronounced among women with a lower dietary intake of vitamin C during pregnancy and among women who smoked during pregnancy. While results are clear in mothers, the association between maternal carcinogenic exposures during pregnancy and biomarkers of early biologic effect in the newborn remains poorly understood.

U2 - 10.1016/j.envres.2015.10.011

DO - 10.1016/j.envres.2015.10.011

M3 - Journal article

C2 - 26496153

SN - 0013-9351

VL - 143

SP - 170

EP - 176

JO - Environmental Research

JF - Environmental Research

IS - Part A

ER -

Outdoor air pollution exposures and micronuclei frequencies in lymphocytes from pregnant women and newborns in Crete, Greece (Rhea cohort)

Abstract

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