Optimizing sampling strategies for NMR-based metabolomics of human feces: pooled vs. unpooled analyses

TY - JOUR

T1 - Optimizing sampling strategies for NMR-based metabolomics of human feces: pooled vs. unpooled analyses

AU - Lamichhane, Santosh

AU - Sundekilde, Ulrik Kræmer

AU - Blædel, Trine

AU - Dalsgaard, Trine Kastrup

AU - Larsen, Lesli Hingstrup

AU - Dragsted, Lars Ove

AU - Astrup, Arne

AU - Bertram, Hanne Christine

N1 - CURIS 2017 NEXS 207

PY - 2017/8/14

Y1 - 2017/8/14

N2 - This study focused on elucidating fecal sample collection for 1H NMR-based metabolite profiling. Pooled vs. unpooled feces sampling was compared to determine if single unpooled samples are adequate and representative for NMR based metabolomics of feces. Our results show that the metabolome of unpooled fecal samples varied within the individual (day-to-day variation). This variation could mainly be ascribed to fluctuations (CV > 40%) in the level of the metabolites glucose, lactate, and succinate. Consequently, our data suggest that multiple sampling and pooling of fecal samples could be considered to minimize errors arising from day to day variation in fecal sample.

AB - This study focused on elucidating fecal sample collection for 1H NMR-based metabolite profiling. Pooled vs. unpooled feces sampling was compared to determine if single unpooled samples are adequate and representative for NMR based metabolomics of feces. Our results show that the metabolome of unpooled fecal samples varied within the individual (day-to-day variation). This variation could mainly be ascribed to fluctuations (CV > 40%) in the level of the metabolites glucose, lactate, and succinate. Consequently, our data suggest that multiple sampling and pooling of fecal samples could be considered to minimize errors arising from day to day variation in fecal sample.

U2 - 10.1039/c7ay01465a

DO - 10.1039/c7ay01465a

M3 - Journal article

SN - 1759-9660

VL - 9

SP - 4476

EP - 4480

JO - Analytical Methods

JF - Analytical Methods

IS - 30

ER -