Optimization of the collection and analysis of touch DNA traces

Hannah T Haase; Helle Smidt Mogensen; Cathrine Bie Petersen; Johan Frederik Torp Petersen; Anders Holmer; Claus Børsting; Vania Pereira

doi:10.1016/j.fsigss.2019.09.038

Optimization of the collection and analysis of touch DNA traces

Hannah T Haase, Helle Smidt Mogensen, Cathrine Bie Petersen, Johan Frederik Torp Petersen, Anders Holmer, Claus Børsting, Vania Pereira^*

^*Corresponding author for this work

Section of Forensic Genetics

3 Citations (Scopus)

Abstract

The aim of this work was to optimize a strategy for detection, collection, and analysis of touch DNA traces. 4N6FLOWSwabs™ and cotton swabs were compared by collecting touch DNA traces from glass slides and gun shell casings. Shed cells were visualized using Diamond™ nucleic acid dye and a digital fluorescent microscope. Different collection and extraction methods were tested. Collected samples were amplified with the AmpFlSTR® NGMSElect™ kit and the Precision ID mtDNA Whole Genome Panel.

Original language	English
Journal	Forensic Science International: Genetics. Supplement Series
Volume	7
ISSN	1875-1768
DOIs	https://doi.org/10.1016/j.fsigss.2019.09.038
Publication status	Published - Dec 2019

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10.1016/j.fsigss.2019.09.038

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title = "Optimization of the collection and analysis of touch DNA traces",

abstract = "The aim of this work was to optimize a strategy for detection, collection, and analysis of touch DNA traces. 4N6FLOWSwabs{\texttrademark} and cotton swabs were compared by collecting touch DNA traces from glass slides and gun shell casings. Shed cells were visualized using Diamond{\texttrademark} nucleic acid dye and a digital fluorescent microscope. Different collection and extraction methods were tested. Collected samples were amplified with the AmpFlSTR{\textregistered} NGMSElect{\texttrademark} kit and the Precision ID mtDNA Whole Genome Panel.",

author = "Haase, {Hannah T} and Mogensen, {Helle Smidt} and Petersen, {Cathrine Bie} and Petersen, {Johan Frederik Torp} and Anders Holmer and Claus B{\o}rsting and Vania Pereira",

year = "2019",

month = dec,

doi = "10.1016/j.fsigss.2019.09.038",

language = "English",

volume = "7",

journal = "Forensic Science International: Genetics. Supplement Series",

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T1 - Optimization of the collection and analysis of touch DNA traces

AU - Haase, Hannah T

AU - Mogensen, Helle Smidt

AU - Petersen, Cathrine Bie

AU - Petersen, Johan Frederik Torp

AU - Holmer, Anders

AU - Børsting, Claus

AU - Pereira, Vania

PY - 2019/12

Y1 - 2019/12

N2 - The aim of this work was to optimize a strategy for detection, collection, and analysis of touch DNA traces. 4N6FLOWSwabs™ and cotton swabs were compared by collecting touch DNA traces from glass slides and gun shell casings. Shed cells were visualized using Diamond™ nucleic acid dye and a digital fluorescent microscope. Different collection and extraction methods were tested. Collected samples were amplified with the AmpFlSTR® NGMSElect™ kit and the Precision ID mtDNA Whole Genome Panel.

AB - The aim of this work was to optimize a strategy for detection, collection, and analysis of touch DNA traces. 4N6FLOWSwabs™ and cotton swabs were compared by collecting touch DNA traces from glass slides and gun shell casings. Shed cells were visualized using Diamond™ nucleic acid dye and a digital fluorescent microscope. Different collection and extraction methods were tested. Collected samples were amplified with the AmpFlSTR® NGMSElect™ kit and the Precision ID mtDNA Whole Genome Panel.

U2 - 10.1016/j.fsigss.2019.09.038

DO - 10.1016/j.fsigss.2019.09.038

M3 - Conference article

SN - 1875-1768

VL - 7

JO - Forensic Science International: Genetics. Supplement Series

JF - Forensic Science International: Genetics. Supplement Series

ER -

Optimization of the collection and analysis of touch DNA traces

Abstract

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