OPEN PULLED STRAW (OPS) VITRIFICATION OF PORCINE BLASTOCYSTS: SIMPLE PROCEDURE YIELDING EXCELLENT IN VITRO SURVIVAL, BUT SO FAR NO PIGLETS FOLLOWING TRANSFER

P Holm; G Vajta; Z Macháty; M Schmidt; R.S. Prather; T Greve; H Callesen

OPEN PULLED STRAW (OPS) VITRIFICATION OF PORCINE BLASTOCYSTS: SIMPLE PROCEDURE YIELDING EXCELLENT IN VITRO SURVIVAL, BUT SO FAR NO PIGLETS FOLLOWING TRANSFER

P Holm, G Vajta, Z Macháty, M Schmidt, R.S. Prather, T Greve, H Callesen

21 Citations (Scopus)

Abstract

In 2 trials, 346 porcine in vivo blastocysts were vitrified in heat-softened and pulled French straws by the Open Pulled Straw (OPS) vitrification method. After thawing, embryos were either cultured in vitro (n=89) for 24 to 48 hours or transferred surgically (n=257) to 11 synchronised recipients without further culture. In trial I. 88% and 71 % of thawed blastocysts reexpanded and hatched in vitro, respectively, while 71 % reexpanded in trial 2. However, no piglets were born following transfer. though 1 recipient was diagnosed pregnant at Day 56 of gestation.

Original language	English
Journal	Cryo-Letters
Issue number	20
Pages (from-to)	307-310
Number of pages	4
ISSN	0143-2044
Publication status	Published - 1999

Cite this

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title = "OPEN PULLED STRAW (OPS) VITRIFICATION OF PORCINE BLASTOCYSTS: SIMPLE PROCEDURE YIELDING EXCELLENT IN VITRO SURVIVAL, BUT SO FAR NO PIGLETS FOLLOWING TRANSFER",

abstract = "In 2 trials, 346 porcine in vivo blastocysts were vitrified in heat-softened and pulled French straws by the Open Pulled Straw (OPS) vitrification method. After thawing, embryos were either cultured in vitro (n=89) for 24 to 48 hours or transferred surgically (n=257) to 11 synchronised recipients without further culture. In trial I. 88% and 71 % of thawed blastocysts reexpanded and hatched in vitro, respectively, while 71 % reexpanded in trial 2. However, no piglets were born following transfer. though 1 recipient was diagnosed pregnant at Day 56 of gestation.",

keywords = "cryopreservation,embryo survival,embryos,swine",

author = "P Holm and G Vajta and Z Mach{\'a}ty and M Schmidt and R.S. Prather and T Greve and H Callesen",

year = "1999",

language = "English",

pages = "307--310",

journal = "Cryo-Letters",

issn = "0143-2044",

publisher = "CryoLetters LLP",

number = "20",

}

TY - JOUR

T1 - OPEN PULLED STRAW (OPS) VITRIFICATION OF PORCINE BLASTOCYSTS: SIMPLE PROCEDURE YIELDING EXCELLENT IN VITRO SURVIVAL, BUT SO FAR NO PIGLETS FOLLOWING TRANSFER

AU - Holm, P

AU - Vajta, G

AU - Macháty, Z

AU - Schmidt, M

AU - Prather, R.S.

AU - Greve, T

AU - Callesen, H

PY - 1999

Y1 - 1999

N2 - In 2 trials, 346 porcine in vivo blastocysts were vitrified in heat-softened and pulled French straws by the Open Pulled Straw (OPS) vitrification method. After thawing, embryos were either cultured in vitro (n=89) for 24 to 48 hours or transferred surgically (n=257) to 11 synchronised recipients without further culture. In trial I. 88% and 71 % of thawed blastocysts reexpanded and hatched in vitro, respectively, while 71 % reexpanded in trial 2. However, no piglets were born following transfer. though 1 recipient was diagnosed pregnant at Day 56 of gestation.

AB - In 2 trials, 346 porcine in vivo blastocysts were vitrified in heat-softened and pulled French straws by the Open Pulled Straw (OPS) vitrification method. After thawing, embryos were either cultured in vitro (n=89) for 24 to 48 hours or transferred surgically (n=257) to 11 synchronised recipients without further culture. In trial I. 88% and 71 % of thawed blastocysts reexpanded and hatched in vitro, respectively, while 71 % reexpanded in trial 2. However, no piglets were born following transfer. though 1 recipient was diagnosed pregnant at Day 56 of gestation.

KW - cryopreservation,embryo survival,embryos,swine

M3 - Journal article

SN - 0143-2044

SP - 307

EP - 310

JO - Cryo-Letters

JF - Cryo-Letters

IS - 20

ER -