On the preparation, microscopic investigation and application of ISCOMs

TY - JOUR

T1 - On the preparation, microscopic investigation and application of ISCOMs

AU - Myschik, Julia

AU - Lendemans, Dirk G

AU - McBurney, Warren T

AU - Demana, Patrick H

AU - Hook, Sarah

AU - Rades, Thomas

PY - 2006

Y1 - 2006

N2 - ISCOM matrices constitute colloidal structures formed from Quillaja saponins, cholesterol and phospholipid. Addition of protein antigens to these matrices leads to the formation of ISCOMs. In this review we report on microscopic investigations of ISCOM matrices and ISCOMs as well as related colloidal structures, such as helices, worm-like micelles, ring-like micelles, and lamellae structures. We briefly outline the immunologic basis for the use of ISCOMs as vaccine delivery systems, and describe the various methods to form ISCOMs. Negative staining transmission electron micrographs of all colloidal structures are presented and described. On the basis of our microscopic investigations, different formation mechanisms of ISCOMS are discussed.

AB - ISCOM matrices constitute colloidal structures formed from Quillaja saponins, cholesterol and phospholipid. Addition of protein antigens to these matrices leads to the formation of ISCOMs. In this review we report on microscopic investigations of ISCOM matrices and ISCOMs as well as related colloidal structures, such as helices, worm-like micelles, ring-like micelles, and lamellae structures. We briefly outline the immunologic basis for the use of ISCOMs as vaccine delivery systems, and describe the various methods to form ISCOMs. Negative staining transmission electron micrographs of all colloidal structures are presented and described. On the basis of our microscopic investigations, different formation mechanisms of ISCOMS are discussed.

U2 - 10.1016/j.micron.2006.03.016

DO - 10.1016/j.micron.2006.03.016

M3 - Journal article

C2 - 16750912

SN - 0968-4328

VL - 37

SP - 724

EP - 734

JO - Micron

JF - Micron

IS - 8

ER -