O-glycosylated cell wall proteins are essential in root hair growth

Silvia M. Velasquez; Martiniano M. Ricardi; Javier Gloazzo Dorosz; Paula V.  Fernandez; Alejandro D.  Nadra; Laercio  Pol-Fachin; Jack Egelund Madsen; Sascha  Gille; Jesper Harholt; Marina  Ciancia; Hugo  Verli; Markus  Pauly; Antony  Bacic; Carl Erik Olsen; Peter Ulvskov; Bent L Petersen; Chris  Somerville; Norberto D.  Iusem; Jose M.  Estevez

doi:10.1126/science.1206657

O-glycosylated cell wall proteins are essential in root hair growth

Silvia M. Velasquez, Martiniano M. Ricardi, Javier Gloazzo Dorosz, Paula V. Fernandez, Alejandro D. Nadra, Laercio Pol-Fachin, Jack Egelund Madsen, Sascha Gille, Jesper Harholt, Marina Ciancia, Hugo Verli, Markus Pauly, Antony Bacic, Carl Erik Olsen, Peter Ulvskov, Bent L Petersen, Chris Somerville, Norberto D. Iusem, Jose M. Estevez

184 Citations (Scopus)

Abstract

Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early posttranslational modification of HRGPs that is catalyzed by prolyl 4-hydroxylases (P4Hs), defines the subsequent O-glycosylation sites in EXTs (which are mainly arabinosylated) and AGPs (which are mainly arabinogalactosylated). We explored the biological function of P4Hs, arabinosyltransferases, and EXTs in root hair cell growth. Biochemical inhibition or genetic disruption resulted in the blockage of polarized growth in root hairs and reduced arabinosylation of EXTs. Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana.

Original language	English
Journal	Science
Volume	332
Issue number	6036
Pages (from-to)	1401-1403
Number of pages	3
ISSN	0036-8075
DOIs	https://doi.org/10.1126/science.1206657
Publication status	Published - 17 Jun 2011

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Velasquez, S. M., Ricardi, M. M., Dorosz, J. G., Fernandez, P. V., Nadra, A. D., Pol-Fachin, L., Madsen, J. E., Gille, S., Harholt, J., Ciancia, M., Verli, H., Pauly, M., Bacic, A., Olsen, C. E., Ulvskov, P., Petersen, B. L., Somerville, C., Iusem, N. D., & Estevez, J. M. (2011). O-glycosylated cell wall proteins are essential in root hair growth. Science, 332(6036), 1401-1403. https://doi.org/10.1126/science.1206657

Velasquez, SM, Ricardi, MM, Dorosz, JG, Fernandez, PV, Nadra, AD, Pol-Fachin, L, Madsen, JE, Gille, S, Harholt, J, Ciancia, M, Verli, H, Pauly, M, Bacic, A, Olsen, CE, Ulvskov, P , Petersen, BL, Somerville, C, Iusem, ND & Estevez, JM 2011, 'O-glycosylated cell wall proteins are essential in root hair growth', Science, vol. 332, no. 6036, pp. 1401-1403. https://doi.org/10.1126/science.1206657

@article{e4d4ddf7c2be41f78d3b40e978e3e94a,

title = "O-glycosylated cell wall proteins are essential in root hair growth",

abstract = "Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early posttranslational modification of HRGPs that is catalyzed by prolyl 4-hydroxylases (P4Hs), defines the subsequent O-glycosylation sites in EXTs (which are mainly arabinosylated) and AGPs (which are mainly arabinogalactosylated). We explored the biological function of P4Hs, arabinosyltransferases, and EXTs in root hair cell growth. Biochemical inhibition or genetic disruption resulted in the blockage of polarized growth in root hairs and reduced arabinosylation of EXTs. Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana.",

author = "Velasquez, {Silvia M.} and Ricardi, {Martiniano M.} and Dorosz, {Javier Gloazzo} and Fernandez, {Paula V.} and Nadra, {Alejandro D.} and Laercio Pol-Fachin and Madsen, {Jack Egelund} and Sascha Gille and Jesper Harholt and Marina Ciancia and Hugo Verli and Markus Pauly and Antony Bacic and Olsen, {Carl Erik} and Peter Ulvskov and Petersen, {Bent L} and Chris Somerville and Iusem, {Norberto D.} and Estevez, {Jose M.}",

year = "2011",

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doi = "10.1126/science.1206657",

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T1 - O-glycosylated cell wall proteins are essential in root hair growth

AU - Velasquez, Silvia M.

AU - Ricardi, Martiniano M.

AU - Dorosz, Javier Gloazzo

AU - Fernandez, Paula V.

AU - Nadra, Alejandro D.

AU - Pol-Fachin, Laercio

AU - Madsen, Jack Egelund

AU - Gille, Sascha

AU - Harholt, Jesper

AU - Ciancia, Marina

AU - Verli, Hugo

AU - Pauly, Markus

AU - Bacic, Antony

AU - Olsen, Carl Erik

AU - Ulvskov, Peter

AU - Petersen, Bent L

AU - Somerville, Chris

AU - Iusem, Norberto D.

AU - Estevez, Jose M.

PY - 2011/6/17

Y1 - 2011/6/17

N2 - Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early posttranslational modification of HRGPs that is catalyzed by prolyl 4-hydroxylases (P4Hs), defines the subsequent O-glycosylation sites in EXTs (which are mainly arabinosylated) and AGPs (which are mainly arabinogalactosylated). We explored the biological function of P4Hs, arabinosyltransferases, and EXTs in root hair cell growth. Biochemical inhibition or genetic disruption resulted in the blockage of polarized growth in root hairs and reduced arabinosylation of EXTs. Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana.

AB - Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early posttranslational modification of HRGPs that is catalyzed by prolyl 4-hydroxylases (P4Hs), defines the subsequent O-glycosylation sites in EXTs (which are mainly arabinosylated) and AGPs (which are mainly arabinogalactosylated). We explored the biological function of P4Hs, arabinosyltransferases, and EXTs in root hair cell growth. Biochemical inhibition or genetic disruption resulted in the blockage of polarized growth in root hairs and reduced arabinosylation of EXTs. Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana.

U2 - 10.1126/science.1206657

DO - 10.1126/science.1206657

M3 - Journal article

C2 - 21680836

SN - 0036-8075

VL - 332

SP - 1401

EP - 1403

JO - Science

JF - Science

IS - 6036

ER -

O-glycosylated cell wall proteins are essential in root hair growth

Abstract

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