TY - BOOK
T1 - Novel Industrial Enzymes from Uncultured Arctic Microorganisms
T2 - Cold- and Alkaline-active Enzymes of Microbial Origin from the Ikaite Columns of SW Greenland
AU - Vester, Jan Kjølhede
PY - 2014
Y1 - 2014
N2 - Many industrial and biotechnological processes make use of cold-active enzymes or could benefit from the use, as the reduced temperature can be beneficial in multiple ways. Such processes may save energy and production costs, improve hygiene, maintain taste and other organoleptic properties, and reduce the risk of contaminations.
Cold- and alkaline-active enzymes can be found in microorganisms adapted to living in natural environments with these conditions, which are extremely rare but found in the unique ikaite columns from SW Greenland (4-6 °C, pH >10).
It is estimated that less than 1% of the microorganisms in an environmental sample can be cultured in the laboratory with standard techniques, which is also the case for the ikaite columns. Thus, there is an enormous potential in the uncultured microorganisms, which cannot be accessed through cultivation based methods.
This PhD thesis presents studies on the diversity of microorganisms from the ikaite columns as well as bioprospecting for enzyme activities using both culture dependent and independent methods. Two cold-active β-galactosidases and one extremely cold-active α-amylase, all related to Clostridia, were characterized in more details.
AB - Many industrial and biotechnological processes make use of cold-active enzymes or could benefit from the use, as the reduced temperature can be beneficial in multiple ways. Such processes may save energy and production costs, improve hygiene, maintain taste and other organoleptic properties, and reduce the risk of contaminations.
Cold- and alkaline-active enzymes can be found in microorganisms adapted to living in natural environments with these conditions, which are extremely rare but found in the unique ikaite columns from SW Greenland (4-6 °C, pH >10).
It is estimated that less than 1% of the microorganisms in an environmental sample can be cultured in the laboratory with standard techniques, which is also the case for the ikaite columns. Thus, there is an enormous potential in the uncultured microorganisms, which cannot be accessed through cultivation based methods.
This PhD thesis presents studies on the diversity of microorganisms from the ikaite columns as well as bioprospecting for enzyme activities using both culture dependent and independent methods. Two cold-active β-galactosidases and one extremely cold-active α-amylase, all related to Clostridia, were characterized in more details.
UR - https://rex.kb.dk/primo-explore/fulldisplay?docid=KGL01008873304&context=L&vid=NUI&search_scope=KGL&tab=default_tab&lang=da_DK
M3 - Ph.D. thesis
BT - Novel Industrial Enzymes from Uncultured Arctic Microorganisms
PB - Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen
ER -