TY - JOUR
T1 - No need for biopsies
T2 - comparison of three sample techniques for wound microbiota determination
AU - Gjødsbøl, Kristine
AU - Skindersoe, Mette E
AU - Christensen, Jens Jørgen
AU - Karlsmark, Tonny
AU - Jørgensen, Bo
AU - Jensen, Anders Mørup
AU - Klein, Bjarke M
AU - Sonnested, Michael K
AU - Krogfelt, Karen
N1 - © 2011 The Authors. © 2011 Blackwell Publishing Ltd and Medicalhelplines.com Inc.
PY - 2012/6
Y1 - 2012/6
N2 - The aim of the study was to compare three sampling techniques used in routine diagnostics to identify the microbiota in chronic venous leg ulcers. A total of 46 patients with persisting venous leg ulcers were included in the study. At inclusion, swab, biopsy and filter paper pad samples were collected. After 4 weeks, additional biopsy and filter paper pad samples were collected. Bacteria were isolated and identified at species level by standard methods. The most common bacterial species detected was Staphylococcus aureus found in 89% of the ulcers. No methicillin-resistant S. aureus isolates were found. We did not find any significant differences regarding the bacterial species isolated between the three sampling techniques. However, using multiple techniques led to identification of more species. Our study suggests that it is sufficient to use swab specimens to identify the bacterial species present in chronic wounds, thus avoiding complications during and after biopsy sampling.
AB - The aim of the study was to compare three sampling techniques used in routine diagnostics to identify the microbiota in chronic venous leg ulcers. A total of 46 patients with persisting venous leg ulcers were included in the study. At inclusion, swab, biopsy and filter paper pad samples were collected. After 4 weeks, additional biopsy and filter paper pad samples were collected. Bacteria were isolated and identified at species level by standard methods. The most common bacterial species detected was Staphylococcus aureus found in 89% of the ulcers. No methicillin-resistant S. aureus isolates were found. We did not find any significant differences regarding the bacterial species isolated between the three sampling techniques. However, using multiple techniques led to identification of more species. Our study suggests that it is sufficient to use swab specimens to identify the bacterial species present in chronic wounds, thus avoiding complications during and after biopsy sampling.
U2 - 10.1111/j.1742-481x.2011.00883.x
DO - 10.1111/j.1742-481x.2011.00883.x
M3 - Journal article
SN - 1742-4801
JO - International Wound Journal
JF - International Wound Journal
ER -