TY - JOUR
T1 - No effect of human serum and erythrocytes enriched in n-3 fatty acids by oral intake on Plasmodium falciparum bloodstage parasites in vitro
AU - Abu-Zeid, Y.A.
AU - Hansen, Harald S.
AU - Jakobsen, P.H.
AU - Bygbjerg, I.C.
AU - Dyerberg, J.
PY - 1993/1/1
Y1 - 1993/1/1
N2 - To examine the effect of n-3 polyunsaturated fatty acids (n-3 PUFA) on the erythrocytic growth of Plasmodium falciparum, serum and erythrocytes were separated from blood of a healthy donor before and after he had taken fish oil capsules for 8 days. Such intake supplied an amount of eicosapentaenoic acid (EPA, 20:5n-3) of 3.5g/d and docosahexaenoic acid (DHA, 22:6n-3) of 2.5 g/d and 24 mg/d of total tocopherol. Post-intake fish oil serum (post-s) and erythrocytes (post-e) were tested in vitro for inhibitory activity against blood stages of P. falciparum compared with pre-intake serum (pre-s) and pre-intake erythrocyte (pre-e). Also the effect of EPA and arachidonic acid (AA, 20:4n-6) on the erythrocytic growth of P. falciparum was tested using in vitro assays. The results show that both post-s and post-e had no antimalarial activity on P. falciparum. No differential antimalarial effect was observed for 20:5n-3 compared with 20:4n-6, which at high concentrations (> 40 µM) had an anti-schizont-growth effect.
AB - To examine the effect of n-3 polyunsaturated fatty acids (n-3 PUFA) on the erythrocytic growth of Plasmodium falciparum, serum and erythrocytes were separated from blood of a healthy donor before and after he had taken fish oil capsules for 8 days. Such intake supplied an amount of eicosapentaenoic acid (EPA, 20:5n-3) of 3.5g/d and docosahexaenoic acid (DHA, 22:6n-3) of 2.5 g/d and 24 mg/d of total tocopherol. Post-intake fish oil serum (post-s) and erythrocytes (post-e) were tested in vitro for inhibitory activity against blood stages of P. falciparum compared with pre-intake serum (pre-s) and pre-intake erythrocyte (pre-e). Also the effect of EPA and arachidonic acid (AA, 20:4n-6) on the erythrocytic growth of P. falciparum was tested using in vitro assays. The results show that both post-s and post-e had no antimalarial activity on P. falciparum. No differential antimalarial effect was observed for 20:5n-3 compared with 20:4n-6, which at high concentrations (> 40 µM) had an anti-schizont-growth effect.
UR - http://www.scopus.com/inward/record.url?scp=0027248559&partnerID=8YFLogxK
M3 - Journal article
AN - SCOPUS:0027248559
SN - 0954-3007
VL - 47
SP - 431
EP - 436
JO - European Journal of Clinical Nutrition
JF - European Journal of Clinical Nutrition
IS - 6
ER -