Next-generation sequencing technology for genetics and genomics of sorghum

TY - CHAP

T1 - Next-generation sequencing technology for genetics and genomics of sorghum

AU - Luo, Hong

AU - Mocoeur, Anne Raymonde Joelle

AU - Jing, Hai-Chun

PY - 2014/1/1

Y1 - 2014/1/1

N2 - The invention and application of Next-Generation Sequencing (NGS) technolgies have revolutionized the study of genetics and genomics. Much research which would not even be considered are nowdays being excuted in many laboratories as routine. In this chapter, we introduce the curretly available NGS platforms, comparing their working theories and reveiwing their advantages and disadvatages. We also discuss the future of NGS development and point out that single molecular sequencing would push the technology to the next level for biological sciences. Much of the chapter focuses on the use of NGS technologies in sorghum. Although the acqusition of the first whole-genome sequence in sorghum was carried out primarily using Sanger sequencing, the use of NGS for examining the genome-wide variation was almost synchronized with other work. Interesting genomic variation was found between sweet and grain sorghum. NGS has also been used to examine the transcriptomes of sorghum under various stress conditions. Besides identifying interesting transcriptonal adptation to stress conditions, these studies show that sugar could potentially act as an osmitc adjusting factor via transcriptional regulation. Furthermore, miRNAs are found to be important for transcriptional adaptation to both biotic and abiotic stresses in sorghum. We discuss the use of NGS for further genetic improvement and breeding of sorghum.

AB - The invention and application of Next-Generation Sequencing (NGS) technolgies have revolutionized the study of genetics and genomics. Much research which would not even be considered are nowdays being excuted in many laboratories as routine. In this chapter, we introduce the curretly available NGS platforms, comparing their working theories and reveiwing their advantages and disadvatages. We also discuss the future of NGS development and point out that single molecular sequencing would push the technology to the next level for biological sciences. Much of the chapter focuses on the use of NGS technologies in sorghum. Although the acqusition of the first whole-genome sequence in sorghum was carried out primarily using Sanger sequencing, the use of NGS for examining the genome-wide variation was almost synchronized with other work. Interesting genomic variation was found between sweet and grain sorghum. NGS has also been used to examine the transcriptomes of sorghum under various stress conditions. Besides identifying interesting transcriptonal adptation to stress conditions, these studies show that sugar could potentially act as an osmitc adjusting factor via transcriptional regulation. Furthermore, miRNAs are found to be important for transcriptional adaptation to both biotic and abiotic stresses in sorghum. We discuss the use of NGS for further genetic improvement and breeding of sorghum.

KW - Former LIFE faculty

KW - Sorghum bicolour L.; Quality Declared Seed System

KW - Next generation sequencing

KW - Genetic

KW - genomic variation

KW - molecular breeding

KW - biofuel-associated traits

KW - Association analysis

M3 - Book chapter

SN - 978-1-4822-1008-8

SP - 226

EP - 250

BT - Genetics, genomics and breeding of sorghum

A2 - Wang, Yi-Hong

A2 - Upadhyaya, Hari D.

A2 - Kole, Chittaranjan

PB - CRC Press

ER -