Next-generation sequencing of whole saliva from patients with primary Sjögren’s syndrome and non-Sjögren’s sicca reveals comparable salivary microbiota

Maria Lynn Sembler-Møller; Daniel Belstrøm; Henning Locht; Christian Enevold; Anne Marie Lynge Pedersen

doi:10.1080/20002297.2019.1660566

Next-generation sequencing of whole saliva from patients with primary Sjögren’s syndrome and non-Sjögren’s sicca reveals comparable salivary microbiota

Maria Lynn Sembler-Møller, Daniel Belstrøm, Henning Locht, Christian Enevold, Anne Marie Lynge Pedersen

Section 01 - Prosthetics

7 Citations (Scopus)

11 Downloads (Pure)

Abstract

Objective: To characterize and compare the salivary microbiota in patients with pSS and patients with non-Sjögren’s-related sicca, and to relate the findings to their oral health status and saliva flow rates. Methods: Twenty-four patients fulfilled the 2016 classification criteria for pSS and 34 did not (non-pSS). A clinical examination included registration of decayed, missing and filled teeth/-surfaces and collection of whole saliva. The microbiota was characterized using next-generation sequencing of the V1–V3 region of the 16S rRNA gene. Data were annotated against the eHOMD database. Results: A total of 509 different bacterial taxa were identified. There were no statistically significant differences between the groups with regard to the abundance of predominant genera, bacterial diversity and relative abundance on the genus or species level. The two groups did not differ with regard to general health, including intake of xerogenic medication and polypharmacy, oral health status or unstimulated and stimulated whole saliva flow rates. Conclusion: The salivary microbiota and oral health status, as well as salivary flow rate in patients with pSS resemble that of non-pSS patients. Our findings indicate that changes in the salivary microbiota do not appear to be determined by the disease entity pSS itself.

Original language	Danish
Article number	1660566
Journal	Journal of Oral Microbiology
Volume	11
Issue number	1
Number of pages	7
ISSN	2000-2297
DOIs	https://doi.org/10.1080/20002297.2019.1660566
Publication status	Published - 2019

Access to Document

10.1080/20002297.2019.1660566Licence: CC BY

Next generation sequencing of whole saliva from patients with primary Sj gren s syndrome and non Sj gren s sicca reveals comparable salivaryFinal published version, 990 KBLicence: CC BY

Cite this

Next-generation sequencing of whole saliva from patients with primary Sjögren’s syndrome and non-Sjögren’s sicca reveals comparable salivary microbiota. / Sembler-Møller, Maria Lynn ; Belstrøm, Daniel; Locht, Henning et al.
In: Journal of Oral Microbiology, Vol. 11, No. 1, 1660566, 2019.

Research output: Contribution to journal › Journal article › Research › peer-review

@article{7eb1fa08130b4906878d71f46c1b8848,

title = "Next-generation sequencing of whole saliva from patients with primary Sj{\"o}gren{\textquoteright}s syndrome and non-Sj{\"o}gren{\textquoteright}s sicca reveals comparable salivary microbiota",

abstract = "Objective: To characterize and compare the salivary microbiota in patients with pSS and patients with non-Sj{\"o}gren{\textquoteright}s-related sicca, and to relate the findings to their oral health status and saliva flow rates. Methods: Twenty-four patients fulfilled the 2016 classification criteria for pSS and 34 did not (non-pSS). A clinical examination included registration of decayed, missing and filled teeth/-surfaces and collection of whole saliva. The microbiota was characterized using next-generation sequencing of the V1–V3 region of the 16S rRNA gene. Data were annotated against the eHOMD database. Results: A total of 509 different bacterial taxa were identified. There were no statistically significant differences between the groups with regard to the abundance of predominant genera, bacterial diversity and relative abundance on the genus or species level. The two groups did not differ with regard to general health, including intake of xerogenic medication and polypharmacy, oral health status or unstimulated and stimulated whole saliva flow rates. Conclusion: The salivary microbiota and oral health status, as well as salivary flow rate in patients with pSS resemble that of non-pSS patients. Our findings indicate that changes in the salivary microbiota do not appear to be determined by the disease entity pSS itself.",

author = "Sembler-M{\o}ller, {Maria Lynn} and Daniel Belstr{\o}m and Henning Locht and Christian Enevold and Pedersen, {Anne Marie Lynge}",

year = "2019",

doi = "10.1080/20002297.2019.1660566",

language = "Dansk",

volume = "11",

journal = "Journal of Oral Microbiology",

issn = "2000-2297",

publisher = "Taylor & Francis",

number = "1",

}

TY - JOUR

T1 - Next-generation sequencing of whole saliva from patients with primary Sjögren’s syndrome and non-Sjögren’s sicca reveals comparable salivary microbiota

AU - Sembler-Møller, Maria Lynn

AU - Belstrøm, Daniel

AU - Locht, Henning

AU - Enevold, Christian

AU - Pedersen, Anne Marie Lynge

PY - 2019

Y1 - 2019

N2 - Objective: To characterize and compare the salivary microbiota in patients with pSS and patients with non-Sjögren’s-related sicca, and to relate the findings to their oral health status and saliva flow rates. Methods: Twenty-four patients fulfilled the 2016 classification criteria for pSS and 34 did not (non-pSS). A clinical examination included registration of decayed, missing and filled teeth/-surfaces and collection of whole saliva. The microbiota was characterized using next-generation sequencing of the V1–V3 region of the 16S rRNA gene. Data were annotated against the eHOMD database. Results: A total of 509 different bacterial taxa were identified. There were no statistically significant differences between the groups with regard to the abundance of predominant genera, bacterial diversity and relative abundance on the genus or species level. The two groups did not differ with regard to general health, including intake of xerogenic medication and polypharmacy, oral health status or unstimulated and stimulated whole saliva flow rates. Conclusion: The salivary microbiota and oral health status, as well as salivary flow rate in patients with pSS resemble that of non-pSS patients. Our findings indicate that changes in the salivary microbiota do not appear to be determined by the disease entity pSS itself.

AB - Objective: To characterize and compare the salivary microbiota in patients with pSS and patients with non-Sjögren’s-related sicca, and to relate the findings to their oral health status and saliva flow rates. Methods: Twenty-four patients fulfilled the 2016 classification criteria for pSS and 34 did not (non-pSS). A clinical examination included registration of decayed, missing and filled teeth/-surfaces and collection of whole saliva. The microbiota was characterized using next-generation sequencing of the V1–V3 region of the 16S rRNA gene. Data were annotated against the eHOMD database. Results: A total of 509 different bacterial taxa were identified. There were no statistically significant differences between the groups with regard to the abundance of predominant genera, bacterial diversity and relative abundance on the genus or species level. The two groups did not differ with regard to general health, including intake of xerogenic medication and polypharmacy, oral health status or unstimulated and stimulated whole saliva flow rates. Conclusion: The salivary microbiota and oral health status, as well as salivary flow rate in patients with pSS resemble that of non-pSS patients. Our findings indicate that changes in the salivary microbiota do not appear to be determined by the disease entity pSS itself.

U2 - 10.1080/20002297.2019.1660566

DO - 10.1080/20002297.2019.1660566

M3 - Tidsskriftartikel

C2 - 31497258

SN - 2000-2297

VL - 11

JO - Journal of Oral Microbiology

JF - Journal of Oral Microbiology

IS - 1

M1 - 1660566

ER -