New insight into erythrocyte through in vivo surface-enhanced Raman spectroscopy

TY - JOUR

T1 - New insight into erythrocyte through in vivo surface-enhanced Raman spectroscopy

AU - Brazhe, Nadezda A

AU - Abdali, Salim

AU - Brazhe, Alexey R

AU - Luneva, Oksana G

AU - Bryzgalova, Nadezda Y

AU - Parshina, Eugenia Y

AU - Sosnovtseva, Olga

AU - Maksimov, Georgy V

N1 - Keywords: Animals; Cell Survival; Cytosol; Erythrocytes; Gold; Hemoglobins; Male; Metal Nanoparticles; Rats; Rats, Wistar; Signal Processing, Computer-Assisted; Spectrum Analysis, Raman; Surface Properties

PY - 2009

Y1 - 2009

N2 - The article presents a noninvasive approach to the study of erythrocyte properties by means of a comparative analysis of signals obtained by surface-enhanced Raman spectroscopy (SERS) and resonance Raman spectroscopy (RS). We report step-by-step the procedure for preparing experimental samples containing erythrocytes in their normal physiological environment in a mixture of colloid solution with silver nanoparticles and the procedure for the optimization of SERS conditions to achieve high signal enhancement without affecting the properties of living erythrocytes. By means of three independent techniques, we demonstrate that under the proposed conditions a colloid solution of silver nanoparticles does not affect the properties of erythrocytes. For the first time to our knowledge, we describe how to use the SERS-RS approach to study two populations of hemoglobin molecules inside an intact living erythrocyte: submembrane and cytosolic hemoglobin (Hb(sm) and Hb(c)). We show that the conformation of Hb(sm) differs from the conformation of Hb(c). This finding has an important application, as the comparative study of Hb(sm) and Hb(c) could be successfully used in biomedical research and diagnostic tests.

AB - The article presents a noninvasive approach to the study of erythrocyte properties by means of a comparative analysis of signals obtained by surface-enhanced Raman spectroscopy (SERS) and resonance Raman spectroscopy (RS). We report step-by-step the procedure for preparing experimental samples containing erythrocytes in their normal physiological environment in a mixture of colloid solution with silver nanoparticles and the procedure for the optimization of SERS conditions to achieve high signal enhancement without affecting the properties of living erythrocytes. By means of three independent techniques, we demonstrate that under the proposed conditions a colloid solution of silver nanoparticles does not affect the properties of erythrocytes. For the first time to our knowledge, we describe how to use the SERS-RS approach to study two populations of hemoglobin molecules inside an intact living erythrocyte: submembrane and cytosolic hemoglobin (Hb(sm) and Hb(c)). We show that the conformation of Hb(sm) differs from the conformation of Hb(c). This finding has an important application, as the comparative study of Hb(sm) and Hb(c) could be successfully used in biomedical research and diagnostic tests.

U2 - 10.1016/j.bpj.2009.09.029

DO - 10.1016/j.bpj.2009.09.029

M3 - Journal article

C2 - 20006958

SN - 0006-3495

VL - 97

SP - 3206

EP - 3214

JO - Biophysical Journal

JF - Biophysical Journal

IS - 12

ER -