Neuronal activity rapidly induces transcription of the CREB-regulated microRNA-132, in vivo

TY - JOUR

T1 - Neuronal activity rapidly induces transcription of the CREB-regulated microRNA-132, in vivo

AU - Nudelman, Aaron Samuel

AU - DiRocco, Derek P

AU - Lambert, Talley J

AU - Garelick, Michael G

AU - Le, Josh

AU - Nathanson, Neil M

AU - Storm, Daniel R

N1 - Keywords: Animals; Association Learning; Behavior, Animal; Cyclic AMP Response Element-Binding Protein; Gene Expression; Hippocampus; Male; Mice; MicroRNAs; Neuronal Plasticity; Neurons; Pilocarpine; RNA, Messenger; Reverse Transcriptase Polymerase Chain Reaction; Seizures; Transcription, Genetic

PY - 2010/4

Y1 - 2010/4

N2 - Activity-dependent changes in gene-expression are believed to underlie the molecular representation of memory. In this study, we report that in vivo activation of neurons rapidly induces the CREB-regulated microRNA miR-132. To determine if production of miR-132 is regulated by neuronal activity its expression in mouse brain was monitored by quantitative RT-PCR (RT-qPCR). Pilocarpine-induced seizures led to a robust, rapid, and transient increase in the primary transcript of miR-132 (pri-miR-132) followed by a subsequent rise in mature microRNA (miR-132). Activation of neurons in the hippocampus, olfactory bulb, and striatum by contextual fear conditioning, odor-exposure, and cocaine-injection, respectively, also increased pri-miR-132. Induction kinetics of pri-miR-132 were monitored and found to parallel those of immediate early genes, peaking at 45 min and returning to basal levels within 2 h of stimulation. Expression levels of primary and maturemiR-132 increased significantly between postnatal Days 10 and 24. We conclude that miR-132 is an activity-dependent microRNA in vivo, and may contribute to the long-lasting proteomic changes required for experience-dependent neuronal plasticity.

AB - Activity-dependent changes in gene-expression are believed to underlie the molecular representation of memory. In this study, we report that in vivo activation of neurons rapidly induces the CREB-regulated microRNA miR-132. To determine if production of miR-132 is regulated by neuronal activity its expression in mouse brain was monitored by quantitative RT-PCR (RT-qPCR). Pilocarpine-induced seizures led to a robust, rapid, and transient increase in the primary transcript of miR-132 (pri-miR-132) followed by a subsequent rise in mature microRNA (miR-132). Activation of neurons in the hippocampus, olfactory bulb, and striatum by contextual fear conditioning, odor-exposure, and cocaine-injection, respectively, also increased pri-miR-132. Induction kinetics of pri-miR-132 were monitored and found to parallel those of immediate early genes, peaking at 45 min and returning to basal levels within 2 h of stimulation. Expression levels of primary and maturemiR-132 increased significantly between postnatal Days 10 and 24. We conclude that miR-132 is an activity-dependent microRNA in vivo, and may contribute to the long-lasting proteomic changes required for experience-dependent neuronal plasticity.

U2 - 10.1002/hipo.20646

DO - 10.1002/hipo.20646

M3 - Journal article

C2 - 19557767

SN - 1050-9631

VL - 20

SP - 492

EP - 498

JO - Hippocampus

JF - Hippocampus

IS - 4

ER -