Neural cell adhesion molecule differentially interacts with isoforms of the fibroblast growth factor receptor

Claus Christensen; Vladimir Berezin; Elisabeth Bock

doi:10.1097/wnr.0b013e3283491682

Neural cell adhesion molecule differentially interacts with isoforms of the fibroblast growth factor receptor

Claus Christensen, Vladimir Berezin, Elisabeth Bock

10 Citations (Scopus)

Abstract

The fibroblast growth factor receptor (FGFR) can be activated through direct interactions with various fibroblast growth factors or through a number of cell adhesion molecules, including the neural cell adhesion molecule (NCAM). We produced recombinant proteins comprising the ligand-binding immunoglobulin-like modules 2 and 3 of FGFR1b, FGFR1c, FGFR2b, FGFR2c, FGFR3b, FGFR3c, and FGFR4, and found that all FGFR isoforms, except for FGFR4, interacted with NCAM. The binding affinity of NCAM-FGFR interactions was considerably higher for splice variant 'b' than for splice variant 'c'. We suggest that the expression pattern of various FGFR isoforms determines the cell context-specific effects of NCAM signaling through FGFR.

Original language	English
Journal	NeuroReport
Volume	22
Issue number	15
Pages (from-to)	727-732
Number of pages	6
ISSN	0959-4965
DOIs	https://doi.org/10.1097/wnr.0b013e3283491682
Publication status	Published - 26 Oct 2011

Keywords

Animals
Male
Neural Cell Adhesion Molecules
Protein Isoforms
Rats
Rats, Wistar
Receptors, Fibroblast Growth Factor
Recombinant Proteins
Signal Transduction
Surface Plasmon Resonance

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10.1097/wnr.0b013e3283491682

Cite this

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title = "Neural cell adhesion molecule differentially interacts with isoforms of the fibroblast growth factor receptor",

abstract = "The fibroblast growth factor receptor (FGFR) can be activated through direct interactions with various fibroblast growth factors or through a number of cell adhesion molecules, including the neural cell adhesion molecule (NCAM). We produced recombinant proteins comprising the ligand-binding immunoglobulin-like modules 2 and 3 of FGFR1b, FGFR1c, FGFR2b, FGFR2c, FGFR3b, FGFR3c, and FGFR4, and found that all FGFR isoforms, except for FGFR4, interacted with NCAM. The binding affinity of NCAM-FGFR interactions was considerably higher for splice variant 'b' than for splice variant 'c'. We suggest that the expression pattern of various FGFR isoforms determines the cell context-specific effects of NCAM signaling through FGFR.",

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year = "2011",

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doi = "10.1097/wnr.0b013e3283491682",

language = "English",

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TY - JOUR

T1 - Neural cell adhesion molecule differentially interacts with isoforms of the fibroblast growth factor receptor

AU - Christensen, Claus

AU - Berezin, Vladimir

AU - Bock, Elisabeth

PY - 2011/10/26

Y1 - 2011/10/26

N2 - The fibroblast growth factor receptor (FGFR) can be activated through direct interactions with various fibroblast growth factors or through a number of cell adhesion molecules, including the neural cell adhesion molecule (NCAM). We produced recombinant proteins comprising the ligand-binding immunoglobulin-like modules 2 and 3 of FGFR1b, FGFR1c, FGFR2b, FGFR2c, FGFR3b, FGFR3c, and FGFR4, and found that all FGFR isoforms, except for FGFR4, interacted with NCAM. The binding affinity of NCAM-FGFR interactions was considerably higher for splice variant 'b' than for splice variant 'c'. We suggest that the expression pattern of various FGFR isoforms determines the cell context-specific effects of NCAM signaling through FGFR.

AB - The fibroblast growth factor receptor (FGFR) can be activated through direct interactions with various fibroblast growth factors or through a number of cell adhesion molecules, including the neural cell adhesion molecule (NCAM). We produced recombinant proteins comprising the ligand-binding immunoglobulin-like modules 2 and 3 of FGFR1b, FGFR1c, FGFR2b, FGFR2c, FGFR3b, FGFR3c, and FGFR4, and found that all FGFR isoforms, except for FGFR4, interacted with NCAM. The binding affinity of NCAM-FGFR interactions was considerably higher for splice variant 'b' than for splice variant 'c'. We suggest that the expression pattern of various FGFR isoforms determines the cell context-specific effects of NCAM signaling through FGFR.

KW - Animals

KW - Male

KW - Neural Cell Adhesion Molecules

KW - Protein Isoforms

KW - Rats

KW - Rats, Wistar

KW - Receptors, Fibroblast Growth Factor

KW - Recombinant Proteins

KW - Signal Transduction

KW - Surface Plasmon Resonance

U2 - 10.1097/wnr.0b013e3283491682

DO - 10.1097/wnr.0b013e3283491682

M3 - Journal article

C2 - 21876469

SN - 0959-4965

VL - 22

SP - 727

EP - 732

JO - NeuroReport

JF - NeuroReport

IS - 15

ER -

Neural cell adhesion molecule differentially interacts with isoforms of the fibroblast growth factor receptor

Abstract

Keywords

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