Neonates colonized with pathogenic bacteria in the airways have a low-grade systemic inflammation

N Rahman Fink; B L Chawes; J. Thorsen; J Stokholm; K A Krogfelt; S Schjørring; M Kragh; K Bønnelykke; S Brix; H Bisgaard

doi:10.1111/all.13461

Neonates colonized with pathogenic bacteria in the airways have a low-grade systemic inflammation

N Rahman Fink, B L Chawes, J. Thorsen, J Stokholm, K A Krogfelt, S Schjørring, M Kragh, K Bønnelykke, S Brix, H Bisgaard

Department of Clinical Medicine

6 Citations (Scopus)

Abstract

Background and objectives: The development of childhood asthma is associated with neonatal colonization with pathogenic bacteria in hypopharynx. Furthermore, established asthma is associated with systemic low-grade inflammation. We here report on the association between neonatal colonization with pathogenic bacteria in hypopharynx and the development of systemic low-grade inflammation. Methods: Bacterial colonization of the hypopharynx with Moraxella catharralis, Haemophilus influenzae, and/or Streptococcus pneumoniae was assessed in asymptomatic children from the Copenhagen Prospective Studies on Asthma in Childhood₂₀₀₀ (COPSAC₂₀₀₀) cohort at age 1 month by culturing technique (N = 238) and by quantitative polymerase chain reaction (qPCR) technique (N = 249) and in the COPSAC₂₀₁₀ cohort by culturing at age 1 month (N = 622) and again at age 3 months (N = 613). Systemic low-grade inflammation was determined in both cohorts at age 6 months by measuring plasma levels of high-sensitivity C-reactive protein (hs-CRP), tumor necrosis factor-α (TNF-α), and interleukin-6 (lL-6). Results: In both cohorts, bacterial colonization was associated with increased levels of hs-CRP: COPSAC₂₀₀₀, 1 month culturing (geometric mean ratio of colonized/noncolonized [95% CI]), 1.39 [0.97-2.01], P =.08; 1 month qPCR, 1.55 [1.14-2.10], P <.01; COPSAC₂₀₁₀, 1 month, 1.52 [1.23-1.87], P <.01; and 3 month, 1.57 [1.30-1.90], P <.01. A multiparametric principal component analysis incorporating hs-CRP, TNF-α, and IL-6 confirmed a systemic inflammatory profile in children colonized with M. catharralis, H. influenzae. and/or S. pneumoniae in the hypopharynx compared to noncolonized children (P-values <.05). Conclusion: The composition of the upper airway microbiome in early life may cause systemic low-grade inflammation.

Original language	English
Journal	Allergy
Volume	73
Issue number	11
Pages (from-to)	2150-2159
Number of pages	10
ISSN	0105-4538
DOIs	https://doi.org/10.1111/all.13461
Publication status	Published - Nov 2018

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@article{814ab67ff73143dba9a90e703ca58757,

title = "Neonates colonized with pathogenic bacteria in the airways have a low-grade systemic inflammation",

abstract = "Background and objectives: The development of childhood asthma is associated with neonatal colonization with pathogenic bacteria in hypopharynx. Furthermore, established asthma is associated with systemic low-grade inflammation. We here report on the association between neonatal colonization with pathogenic bacteria in hypopharynx and the development of systemic low-grade inflammation. Methods: Bacterial colonization of the hypopharynx with Moraxella catharralis, Haemophilus influenzae, and/or Streptococcus pneumoniae was assessed in asymptomatic children from the Copenhagen Prospective Studies on Asthma in Childhood2000 (COPSAC2000) cohort at age 1 month by culturing technique (N = 238) and by quantitative polymerase chain reaction (qPCR) technique (N = 249) and in the COPSAC2010 cohort by culturing at age 1 month (N = 622) and again at age 3 months (N = 613). Systemic low-grade inflammation was determined in both cohorts at age 6 months by measuring plasma levels of high-sensitivity C-reactive protein (hs-CRP), tumor necrosis factor-α (TNF-α), and interleukin-6 (lL-6). Results: In both cohorts, bacterial colonization was associated with increased levels of hs-CRP: COPSAC2000, 1 month culturing (geometric mean ratio of colonized/noncolonized [95% CI]), 1.39 [0.97-2.01], P =.08; 1 month qPCR, 1.55 [1.14-2.10], P <.01; COPSAC2010, 1 month, 1.52 [1.23-1.87], P <.01; and 3 month, 1.57 [1.30-1.90], P <.01. A multiparametric principal component analysis incorporating hs-CRP, TNF-α, and IL-6 confirmed a systemic inflammatory profile in children colonized with M. catharralis, H. influenzae. and/or S. pneumoniae in the hypopharynx compared to noncolonized children (P-values <.05). Conclusion: The composition of the upper airway microbiome in early life may cause systemic low-grade inflammation.",

author = "{Rahman Fink}, N and Chawes, {B L} and J. Thorsen and J Stokholm and Krogfelt, {K A} and S Schj{\o}rring and M Kragh and K B{\o}nnelykke and S Brix and H Bisgaard",

note = "{\textcopyright} 2018 EAACI and John Wiley and Sons A/S. Published by John Wiley and Sons Ltd.",

year = "2018",

month = nov,

doi = "10.1111/all.13461",

language = "English",

volume = "73",

pages = "2150--2159",

journal = "Allergy: European Journal of Allergy and Clinical Immunology",

issn = "0105-4538",

publisher = "Wiley Online",

number = "11",

}

TY - JOUR

T1 - Neonates colonized with pathogenic bacteria in the airways have a low-grade systemic inflammation

AU - Rahman Fink, N

AU - Chawes, B L

AU - Thorsen, J.

AU - Stokholm, J

AU - Krogfelt, K A

AU - Schjørring, S

AU - Kragh, M

AU - Bønnelykke, K

AU - Brix, S

AU - Bisgaard, H

PY - 2018/11

Y1 - 2018/11

N2 - Background and objectives: The development of childhood asthma is associated with neonatal colonization with pathogenic bacteria in hypopharynx. Furthermore, established asthma is associated with systemic low-grade inflammation. We here report on the association between neonatal colonization with pathogenic bacteria in hypopharynx and the development of systemic low-grade inflammation. Methods: Bacterial colonization of the hypopharynx with Moraxella catharralis, Haemophilus influenzae, and/or Streptococcus pneumoniae was assessed in asymptomatic children from the Copenhagen Prospective Studies on Asthma in Childhood2000 (COPSAC2000) cohort at age 1 month by culturing technique (N = 238) and by quantitative polymerase chain reaction (qPCR) technique (N = 249) and in the COPSAC2010 cohort by culturing at age 1 month (N = 622) and again at age 3 months (N = 613). Systemic low-grade inflammation was determined in both cohorts at age 6 months by measuring plasma levels of high-sensitivity C-reactive protein (hs-CRP), tumor necrosis factor-α (TNF-α), and interleukin-6 (lL-6). Results: In both cohorts, bacterial colonization was associated with increased levels of hs-CRP: COPSAC2000, 1 month culturing (geometric mean ratio of colonized/noncolonized [95% CI]), 1.39 [0.97-2.01], P =.08; 1 month qPCR, 1.55 [1.14-2.10], P <.01; COPSAC2010, 1 month, 1.52 [1.23-1.87], P <.01; and 3 month, 1.57 [1.30-1.90], P <.01. A multiparametric principal component analysis incorporating hs-CRP, TNF-α, and IL-6 confirmed a systemic inflammatory profile in children colonized with M. catharralis, H. influenzae. and/or S. pneumoniae in the hypopharynx compared to noncolonized children (P-values <.05). Conclusion: The composition of the upper airway microbiome in early life may cause systemic low-grade inflammation.

AB - Background and objectives: The development of childhood asthma is associated with neonatal colonization with pathogenic bacteria in hypopharynx. Furthermore, established asthma is associated with systemic low-grade inflammation. We here report on the association between neonatal colonization with pathogenic bacteria in hypopharynx and the development of systemic low-grade inflammation. Methods: Bacterial colonization of the hypopharynx with Moraxella catharralis, Haemophilus influenzae, and/or Streptococcus pneumoniae was assessed in asymptomatic children from the Copenhagen Prospective Studies on Asthma in Childhood2000 (COPSAC2000) cohort at age 1 month by culturing technique (N = 238) and by quantitative polymerase chain reaction (qPCR) technique (N = 249) and in the COPSAC2010 cohort by culturing at age 1 month (N = 622) and again at age 3 months (N = 613). Systemic low-grade inflammation was determined in both cohorts at age 6 months by measuring plasma levels of high-sensitivity C-reactive protein (hs-CRP), tumor necrosis factor-α (TNF-α), and interleukin-6 (lL-6). Results: In both cohorts, bacterial colonization was associated with increased levels of hs-CRP: COPSAC2000, 1 month culturing (geometric mean ratio of colonized/noncolonized [95% CI]), 1.39 [0.97-2.01], P =.08; 1 month qPCR, 1.55 [1.14-2.10], P <.01; COPSAC2010, 1 month, 1.52 [1.23-1.87], P <.01; and 3 month, 1.57 [1.30-1.90], P <.01. A multiparametric principal component analysis incorporating hs-CRP, TNF-α, and IL-6 confirmed a systemic inflammatory profile in children colonized with M. catharralis, H. influenzae. and/or S. pneumoniae in the hypopharynx compared to noncolonized children (P-values <.05). Conclusion: The composition of the upper airway microbiome in early life may cause systemic low-grade inflammation.

U2 - 10.1111/all.13461

DO - 10.1111/all.13461

M3 - Journal article

C2 - 29672858

SN - 0105-4538

VL - 73

SP - 2150

EP - 2159

JO - Allergy: European Journal of Allergy and Clinical Immunology

JF - Allergy: European Journal of Allergy and Clinical Immunology

IS - 11

ER -

Neonates colonized with pathogenic bacteria in the airways have a low-grade systemic inflammation

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