Neonatal microbial colonization in mice promotes prolonged dominance of CD11b+Gr-1+ cells and accelerated establishment of the CD4+ T cell population in the spleen

Matilde B Kristensen; Stine Broeng Metzdorff; Anders Bergström; Dina Silke Malling Damlund; Lisbeth N Fink; Tine R Licht; Hanne Frøkiær

doi:10.1002/iid3.70

Neonatal microbial colonization in mice promotes prolonged dominance of CD11b⁺Gr-1⁺ cells and accelerated establishment of the CD4⁺ T cell population in the spleen

Matilde B Kristensen, Stine Broeng Metzdorff, Anders Bergström, Dina Silke Malling Damlund, Lisbeth N Fink, Tine R Licht, Hanne Frøkiær

9 Citations (Scopus)

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Abstract

To assess the microbial influence on postnatal hematopoiesis, we examined the role of early life microbial colonization on the composition of leukocyte subsets in the neonatal spleen. A high number of CD11b(+)Gr-1(+) splenocytes present perinatally was sustained for a longer period in conventionally colonized (CONV) mice than in mono-colonized (MC) and germfree (GF) mice, and the CD4(+) T cell population established faster in CONV mice. At the day of birth, compared to GF mice, the expression of Cxcl2 was up-regulated and Arg1 down-regulated in livers of CONV mice. This coincided with lower abundance of polylobed cells in the liver of CONV mice. An earlier peak in the expression of the genes Tjp1, Cdh1, and JamA in intestinal epithelial cells of CONV mice indicated an accelerated closure of the epithelial barrier. In conclusion, we have identified an important microbiota-dependent neonatal hematopoietic event, which we suggest impacts the subsequent development of the T cell population in the murine spleen.

Original language	English
Journal	Immunity, Inflammation and Disease
Volume	3
Issue number	3
Pages (from-to)	309-320
Number of pages	12
ISSN	2050-4527
DOIs	https://doi.org/10.1002/iid3.70
Publication status	Published - Sept 2015

Keywords

Faculty of Health and Medical Sciences

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Neonatal microbial colonization in mice promotes prolonged dominance of CD11b+Gr-1+ cells and accelerated establishment of the CD4+ T cell population in the spleenFinal published version, 1.72 MB
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Kristensen, M. B., Metzdorff, S. B., Bergström, A., Damlund, D. S. M., Fink, L. N., Licht, T. R., & Frøkiær, H. (2015). Neonatal microbial colonization in mice promotes prolonged dominance of CD11b⁺Gr-1⁺ cells and accelerated establishment of the CD4⁺ T cell population in the spleen. Immunity, Inflammation and Disease, 3(3), 309-320. https://doi.org/10.1002/iid3.70

Neonatal microbial colonization in mice promotes prolonged dominance of CD11b⁺Gr-1⁺ cells and accelerated establishment of the CD4⁺ T cell population in the spleen. / Kristensen, Matilde B; Metzdorff, Stine Broeng; Bergström, Anders et al.
In: Immunity, Inflammation and Disease, Vol. 3, No. 3, 09.2015, p. 309-320.

Research output: Contribution to journal › Journal article › Research › peer-review

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abstract = "To assess the microbial influence on postnatal hematopoiesis, we examined the role of early life microbial colonization on the composition of leukocyte subsets in the neonatal spleen. A high number of CD11b(+)Gr-1(+) splenocytes present perinatally was sustained for a longer period in conventionally colonized (CONV) mice than in mono-colonized (MC) and germfree (GF) mice, and the CD4(+) T cell population established faster in CONV mice. At the day of birth, compared to GF mice, the expression of Cxcl2 was up-regulated and Arg1 down-regulated in livers of CONV mice. This coincided with lower abundance of polylobed cells in the liver of CONV mice. An earlier peak in the expression of the genes Tjp1, Cdh1, and JamA in intestinal epithelial cells of CONV mice indicated an accelerated closure of the epithelial barrier. In conclusion, we have identified an important microbiota-dependent neonatal hematopoietic event, which we suggest impacts the subsequent development of the T cell population in the murine spleen.",

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AU - Metzdorff, Stine Broeng

AU - Bergström, Anders

AU - Damlund, Dina Silke Malling

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AU - Licht, Tine R

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AB - To assess the microbial influence on postnatal hematopoiesis, we examined the role of early life microbial colonization on the composition of leukocyte subsets in the neonatal spleen. A high number of CD11b(+)Gr-1(+) splenocytes present perinatally was sustained for a longer period in conventionally colonized (CONV) mice than in mono-colonized (MC) and germfree (GF) mice, and the CD4(+) T cell population established faster in CONV mice. At the day of birth, compared to GF mice, the expression of Cxcl2 was up-regulated and Arg1 down-regulated in livers of CONV mice. This coincided with lower abundance of polylobed cells in the liver of CONV mice. An earlier peak in the expression of the genes Tjp1, Cdh1, and JamA in intestinal epithelial cells of CONV mice indicated an accelerated closure of the epithelial barrier. In conclusion, we have identified an important microbiota-dependent neonatal hematopoietic event, which we suggest impacts the subsequent development of the T cell population in the murine spleen.

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