NADPH-dependent thioredoxin reductase and 2-Cys peroxiredoxins are needed for the protection of Mg-protoporphyrin monomethyl ester cyclase

Anne Stenbæk; Andreas Hansson; Ragna Peterson Wulff; Mats Hansson; Karl-Josef Dietz; Poul Erik Jensen

doi:10.1016/j.febslet.2008.07.006

NADPH-dependent thioredoxin reductase and 2-Cys peroxiredoxins are needed for the protection of Mg-protoporphyrin monomethyl ester cyclase

Anne Stenbæk, Andreas Hansson, Ragna Peterson Wulff, Mats Hansson, Karl-Josef Dietz, Poul Erik Jensen

76 Citations (Scopus)

Abstract

Abstract: The chloroplast-localized NADPH-dependent thioredoxin reductase ( NTRC) has been found to be able to reduce hydrogen peroxide scavenging 2-Cys peroxiredoxins. We show that the Arabidopsis ntrc mutant is perturbed in chlorophyll biosynthesis and accumulate intermediates preceding protochlorophyllide formation. A specific involvement of NTRC during biosynthesis of protochlorophyllide is indicated from in vitro aerobic cyclase assays in which the conversion of Mg-protoporhyrin monomethyl ester into protochlorophyllide is stimulated by addition of the NTRC/2-Cys peroxiredoxin system. These findings support the hypothesis that this NADPH-dependent hydrogen peroxide scavenging system is particularly important during periods with limited reducing power from photosynthesis, e. g. under chloroplast biogenesis. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Original language	English
Journal	FEBS Letters
Volume	582
Issue number	18
Pages (from-to)	2773-2778
Number of pages	6
ISSN	0014-5793
DOIs	https://doi.org/10.1016/j.febslet.2008.07.006
Publication status	Published - 2008

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10.1016/j.febslet.2008.07.006

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title = "NADPH-dependent thioredoxin reductase and 2-Cys peroxiredoxins are needed for the protection of Mg-protoporphyrin monomethyl ester cyclase",

abstract = "Abstract: The chloroplast-localized NADPH-dependent thioredoxin reductase ( NTRC) has been found to be able to reduce hydrogen peroxide scavenging 2-Cys peroxiredoxins. We show that the Arabidopsis ntrc mutant is perturbed in chlorophyll biosynthesis and accumulate intermediates preceding protochlorophyllide formation. A specific involvement of NTRC during biosynthesis of protochlorophyllide is indicated from in vitro aerobic cyclase assays in which the conversion of Mg-protoporhyrin monomethyl ester into protochlorophyllide is stimulated by addition of the NTRC/2-Cys peroxiredoxin system. These findings support the hypothesis that this NADPH-dependent hydrogen peroxide scavenging system is particularly important during periods with limited reducing power from photosynthesis, e. g. under chloroplast biogenesis. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.",

author = "Anne Stenb{\ae}k and Andreas Hansson and Wulff, {Ragna Peterson} and Mats Hansson and Karl-Josef Dietz and Jensen, {Poul Erik}",

year = "2008",

doi = "10.1016/j.febslet.2008.07.006",

language = "English",

volume = "582",

pages = "2773--2778",

journal = "F E B S Letters",

issn = "0014-5793",

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TY - JOUR

T1 - NADPH-dependent thioredoxin reductase and 2-Cys peroxiredoxins are needed for the protection of Mg-protoporphyrin monomethyl ester cyclase

AU - Stenbæk, Anne

AU - Hansson, Andreas

AU - Wulff, Ragna Peterson

AU - Hansson, Mats

AU - Dietz, Karl-Josef

AU - Jensen, Poul Erik

PY - 2008

Y1 - 2008

N2 - Abstract: The chloroplast-localized NADPH-dependent thioredoxin reductase ( NTRC) has been found to be able to reduce hydrogen peroxide scavenging 2-Cys peroxiredoxins. We show that the Arabidopsis ntrc mutant is perturbed in chlorophyll biosynthesis and accumulate intermediates preceding protochlorophyllide formation. A specific involvement of NTRC during biosynthesis of protochlorophyllide is indicated from in vitro aerobic cyclase assays in which the conversion of Mg-protoporhyrin monomethyl ester into protochlorophyllide is stimulated by addition of the NTRC/2-Cys peroxiredoxin system. These findings support the hypothesis that this NADPH-dependent hydrogen peroxide scavenging system is particularly important during periods with limited reducing power from photosynthesis, e. g. under chloroplast biogenesis. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

AB - Abstract: The chloroplast-localized NADPH-dependent thioredoxin reductase ( NTRC) has been found to be able to reduce hydrogen peroxide scavenging 2-Cys peroxiredoxins. We show that the Arabidopsis ntrc mutant is perturbed in chlorophyll biosynthesis and accumulate intermediates preceding protochlorophyllide formation. A specific involvement of NTRC during biosynthesis of protochlorophyllide is indicated from in vitro aerobic cyclase assays in which the conversion of Mg-protoporhyrin monomethyl ester into protochlorophyllide is stimulated by addition of the NTRC/2-Cys peroxiredoxin system. These findings support the hypothesis that this NADPH-dependent hydrogen peroxide scavenging system is particularly important during periods with limited reducing power from photosynthesis, e. g. under chloroplast biogenesis. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

U2 - 10.1016/j.febslet.2008.07.006

DO - 10.1016/j.febslet.2008.07.006

M3 - Journal article

SN - 0014-5793

VL - 582

SP - 2773

EP - 2778

JO - F E B S Letters

JF - F E B S Letters

IS - 18

ER -

NADPH-dependent thioredoxin reductase and 2-Cys peroxiredoxins are needed for the protection of Mg-protoporphyrin monomethyl ester cyclase

Abstract

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