Myocardial perfusion of infarcted and normal myocardium in propofol-anesthetized minipigs using 82Rubidium PET

Thomas Rasmussen; Bjarke Follin Larsen; Jens Kastrup; Thomas Emil Christensen; Karsten Pharao Hammelev; Andreas Kjær; Philip Hasbak

doi:10.1007/s12350-016-0453-z

Myocardial perfusion of infarcted and normal myocardium in propofol-anesthetized minipigs using ⁸²Rubidium PET

Thomas Rasmussen^*, Bjarke Follin Larsen, Jens Kastrup, Thomas Emil Christensen, Karsten Pharao Hammelev, Andreas Kjær, Philip Hasbak

^*Corresponding author for this work

1 Citation (Scopus)

Abstract

Cardiac Rubidium-82 (⁸²Rb) positron-emission-tomography (PET) is a good method for quantification of myocardial blood flow in man. Quantification of myocardial blood flow in animals to evaluate new treatment strategies or to understand underlying disease is also of great interest but raises some challenges. Animals, which have been anesthetized during PET acquisition, might react differently to used stress medications, and therefore difficulties might exist while evaluating the resulting PET images using standard software packages from commercial vendors optimized for human hearts. Furthermore propofol, used for anesthesia, can influence myocardial perfusion and coronary flow reserve due to its vasorelaxant effect, and interactions might exist between propofol and used stress agents, potentially affecting the result of the examination. We present cardiac ⁸²Rb-PET studies performed in propofol-anesthetized minipigs with normal and infarcted myocardium stressed with both adenosine and dipyridamole. Despite the mentioned challenges, we were able to trace the small minipig heart with software designed for human cardiac PET and to achieve blood flow measurements comparable with results in humans with both adenosine and dipyridamole. We found dipyridamole to be a superior stress agent for this experimental setup. Finally, we were able to clearly identify the myocardial perfusion defect after an induced myocardial infarction.

Original language	English
Journal	Journal of Nuclear Cardiology
Volume	23
Issue number	3
Pages (from-to)	599-603
Number of pages	5
ISSN	1071-3581
DOIs	https://doi.org/10.1007/s12350-016-0453-z
Publication status	Published - 1 Jun 2016

Keywords

A2A adenosine receptor agonists
coronary artery disease
infarction
myocardial
myocardial perfusion imaging
PET
pharmacologic stress

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10.1007/s12350-016-0453-z

Cite this

@article{4ffa19990074459dba6b20f11f6f9573,

title = "Myocardial perfusion of infarcted and normal myocardium in propofol-anesthetized minipigs using 82Rubidium PET",

abstract = "Cardiac Rubidium-82 (82Rb) positron-emission-tomography (PET) is a good method for quantification of myocardial blood flow in man. Quantification of myocardial blood flow in animals to evaluate new treatment strategies or to understand underlying disease is also of great interest but raises some challenges. Animals, which have been anesthetized during PET acquisition, might react differently to used stress medications, and therefore difficulties might exist while evaluating the resulting PET images using standard software packages from commercial vendors optimized for human hearts. Furthermore propofol, used for anesthesia, can influence myocardial perfusion and coronary flow reserve due to its vasorelaxant effect, and interactions might exist between propofol and used stress agents, potentially affecting the result of the examination. We present cardiac 82Rb-PET studies performed in propofol-anesthetized minipigs with normal and infarcted myocardium stressed with both adenosine and dipyridamole. Despite the mentioned challenges, we were able to trace the small minipig heart with software designed for human cardiac PET and to achieve blood flow measurements comparable with results in humans with both adenosine and dipyridamole. We found dipyridamole to be a superior stress agent for this experimental setup. Finally, we were able to clearly identify the myocardial perfusion defect after an induced myocardial infarction.",

keywords = "A2A adenosine receptor agonists, coronary artery disease, infarction, myocardial, myocardial perfusion imaging, PET, pharmacologic stress",

author = "Thomas Rasmussen and Larsen, {Bjarke Follin} and Jens Kastrup and Christensen, {Thomas Emil} and Hammelev, {Karsten Pharao} and Andreas Kj{\ae}r and Philip Hasbak",

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T1 - Myocardial perfusion of infarcted and normal myocardium in propofol-anesthetized minipigs using 82Rubidium PET

AU - Rasmussen, Thomas

AU - Larsen, Bjarke Follin

AU - Kastrup, Jens

AU - Christensen, Thomas Emil

AU - Hammelev, Karsten Pharao

AU - Kjær, Andreas

AU - Hasbak, Philip

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Cardiac Rubidium-82 (82Rb) positron-emission-tomography (PET) is a good method for quantification of myocardial blood flow in man. Quantification of myocardial blood flow in animals to evaluate new treatment strategies or to understand underlying disease is also of great interest but raises some challenges. Animals, which have been anesthetized during PET acquisition, might react differently to used stress medications, and therefore difficulties might exist while evaluating the resulting PET images using standard software packages from commercial vendors optimized for human hearts. Furthermore propofol, used for anesthesia, can influence myocardial perfusion and coronary flow reserve due to its vasorelaxant effect, and interactions might exist between propofol and used stress agents, potentially affecting the result of the examination. We present cardiac 82Rb-PET studies performed in propofol-anesthetized minipigs with normal and infarcted myocardium stressed with both adenosine and dipyridamole. Despite the mentioned challenges, we were able to trace the small minipig heart with software designed for human cardiac PET and to achieve blood flow measurements comparable with results in humans with both adenosine and dipyridamole. We found dipyridamole to be a superior stress agent for this experimental setup. Finally, we were able to clearly identify the myocardial perfusion defect after an induced myocardial infarction.

AB - Cardiac Rubidium-82 (82Rb) positron-emission-tomography (PET) is a good method for quantification of myocardial blood flow in man. Quantification of myocardial blood flow in animals to evaluate new treatment strategies or to understand underlying disease is also of great interest but raises some challenges. Animals, which have been anesthetized during PET acquisition, might react differently to used stress medications, and therefore difficulties might exist while evaluating the resulting PET images using standard software packages from commercial vendors optimized for human hearts. Furthermore propofol, used for anesthesia, can influence myocardial perfusion and coronary flow reserve due to its vasorelaxant effect, and interactions might exist between propofol and used stress agents, potentially affecting the result of the examination. We present cardiac 82Rb-PET studies performed in propofol-anesthetized minipigs with normal and infarcted myocardium stressed with both adenosine and dipyridamole. Despite the mentioned challenges, we were able to trace the small minipig heart with software designed for human cardiac PET and to achieve blood flow measurements comparable with results in humans with both adenosine and dipyridamole. We found dipyridamole to be a superior stress agent for this experimental setup. Finally, we were able to clearly identify the myocardial perfusion defect after an induced myocardial infarction.

KW - A2A adenosine receptor agonists

KW - coronary artery disease

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KW - myocardial

KW - myocardial perfusion imaging

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KW - pharmacologic stress

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