MYB75 phosphorylation by MPK4 is required for light-induced anthocyanin accumulation in arabidopsis

Shengnan Li; Wenyi Wang; Jinlan Gao; Kangquan Yin; Rui Wang; Chengcheng Wang; Morten Petersen; John Mundy; Jin-Long Qiu

doi:10.1105/tpc.16.00130

MYB75 phosphorylation by MPK4 is required for light-induced anthocyanin accumulation in arabidopsis

Shengnan Li, Wenyi Wang, Jinlan Gao, Kangquan Yin, Rui Wang, Chengcheng Wang, Morten Petersen, John Mundy, Jin-Long Qiu

Functional Genomics

68 Citations (Scopus)

Abstract

Light is a major environmental cue affecting various physiological and metabolic processes in plants. Although plant photoreceptors are well characterized, the mechanisms by which light regulates downstream responses are less clear. In Arabidopsis thaliana, the accumulation of photoprotective anthocyanin pigments is light dependent, and the R2R3 MYB transcription factor MYB75/PAP1 regulates anthocyanin accumulation. Here, we report that MYB75 interacts with and is phosphorylated by MAP KINASE4 (MPK4). Their interaction is dependent on MPK4 kinase activity and is required for full function of MYB75. MPK4 can be activated in response to light and is involved in the light-induced accumulation of anthocyanins. We show that MPK4 phosphorylation of MYB75 increases its stability and is essential for light-induced anthocyanin accumulation. Our findings reveal an important role for a MAPK pathway in light signal transduction.

Original language	English
Journal	The Plant Cell
Volume	28
Issue number	11
Pages (from-to)	2866-2883
Number of pages	18
ISSN	1040-4651
DOIs	https://doi.org/10.1105/tpc.16.00130
Publication status	Published - Nov 2016

Access to Document

10.1105/tpc.16.00130

2866.full.pdfFinal published version, 2.45 MB

http://www.plantcell.org/content/plantcell/28/11/2866.full.pdf

Cite this

@article{75f527303b2042acb4a462b78eaaff08,

title = "MYB75 phosphorylation by MPK4 is required for light-induced anthocyanin accumulation in arabidopsis",

abstract = "Light is a major environmental cue affecting various physiological and metabolic processes in plants. Although plant photoreceptors are well characterized, the mechanisms by which light regulates downstream responses are less clear. In Arabidopsis thaliana, the accumulation of photoprotective anthocyanin pigments is light dependent, and the R2R3 MYB transcription factor MYB75/PAP1 regulates anthocyanin accumulation. Here, we report that MYB75 interacts with and is phosphorylated by MAP KINASE4 (MPK4). Their interaction is dependent on MPK4 kinase activity and is required for full function of MYB75. MPK4 can be activated in response to light and is involved in the light-induced accumulation of anthocyanins. We show that MPK4 phosphorylation of MYB75 increases its stability and is essential for light-induced anthocyanin accumulation. Our findings reveal an important role for a MAPK pathway in light signal transduction.",

author = "Shengnan Li and Wenyi Wang and Jinlan Gao and Kangquan Yin and Rui Wang and Chengcheng Wang and Morten Petersen and John Mundy and Jin-Long Qiu",

year = "2016",

month = nov,

doi = "10.1105/tpc.16.00130",

language = "English",

volume = "28",

pages = "2866--2883",

journal = "The Plant Cell",

issn = "1040-4651",

publisher = "American Society of Plant Biologists",

number = "11",

}

TY - JOUR

T1 - MYB75 phosphorylation by MPK4 is required for light-induced anthocyanin accumulation in arabidopsis

AU - Li, Shengnan

AU - Wang, Wenyi

AU - Gao, Jinlan

AU - Yin, Kangquan

AU - Wang, Rui

AU - Wang, Chengcheng

AU - Petersen, Morten

AU - Mundy, John

AU - Qiu, Jin-Long

PY - 2016/11

Y1 - 2016/11

N2 - Light is a major environmental cue affecting various physiological and metabolic processes in plants. Although plant photoreceptors are well characterized, the mechanisms by which light regulates downstream responses are less clear. In Arabidopsis thaliana, the accumulation of photoprotective anthocyanin pigments is light dependent, and the R2R3 MYB transcription factor MYB75/PAP1 regulates anthocyanin accumulation. Here, we report that MYB75 interacts with and is phosphorylated by MAP KINASE4 (MPK4). Their interaction is dependent on MPK4 kinase activity and is required for full function of MYB75. MPK4 can be activated in response to light and is involved in the light-induced accumulation of anthocyanins. We show that MPK4 phosphorylation of MYB75 increases its stability and is essential for light-induced anthocyanin accumulation. Our findings reveal an important role for a MAPK pathway in light signal transduction.

AB - Light is a major environmental cue affecting various physiological and metabolic processes in plants. Although plant photoreceptors are well characterized, the mechanisms by which light regulates downstream responses are less clear. In Arabidopsis thaliana, the accumulation of photoprotective anthocyanin pigments is light dependent, and the R2R3 MYB transcription factor MYB75/PAP1 regulates anthocyanin accumulation. Here, we report that MYB75 interacts with and is phosphorylated by MAP KINASE4 (MPK4). Their interaction is dependent on MPK4 kinase activity and is required for full function of MYB75. MPK4 can be activated in response to light and is involved in the light-induced accumulation of anthocyanins. We show that MPK4 phosphorylation of MYB75 increases its stability and is essential for light-induced anthocyanin accumulation. Our findings reveal an important role for a MAPK pathway in light signal transduction.

U2 - 10.1105/tpc.16.00130

DO - 10.1105/tpc.16.00130

M3 - Journal article

C2 - 27811015

SN - 1040-4651

VL - 28

SP - 2866

EP - 2883

JO - The Plant Cell

JF - The Plant Cell

IS - 11

ER -

MYB75 phosphorylation by MPK4 is required for light-induced anthocyanin accumulation in arabidopsis

Abstract

Access to Document

Fingerprint

Cite this