Mutation of a bHLH transcription factor allowed almond domestication

R. Sánchez-Pérez; S. Pavan; R. Mazzeo; C. Moldovan; R. Aiese Cigliano; J. Del Cueto; F. Ricciardi; C. Lotti; L. Ricciardi; F. Dicenta; R. L. López-Marqués; B. Lindberg Møller

doi:10.1126/science.aav8197

Mutation of a bHLH transcription factor allowed almond domestication

R. Sánchez-Pérez^*, S. Pavan, R. Mazzeo, C. Moldovan, R. Aiese Cigliano, J. Del Cueto, F. Ricciardi, C. Lotti, L. Ricciardi, F. Dicenta, R. L. López-Marqués, B. Lindberg Møller

^*Corresponding author for this work

45 Citations (Scopus)

Abstract

Wild almond species accumulate the bitter and toxic cyanogenic diglucoside amygdalin. Almond domestication was enabled by the selection of genotypes harboring sweet kernels. We report the completion of the almond reference genome. Map-based cloning using an F₁ population segregating for kernel taste led to the identification of a 46-kilobase gene cluster encoding five basic helix-loop-helix transcription factors, bHLH1 to bHLH5. Functional characterization demonstrated that bHLH2 controls transcription of the P450 monooxygenase–encoding genes PdCYP79D16 and PdCYP71AN24, which are involved in the amygdalin biosynthetic pathway. A nonsynonymous point mutation (Leu to Phe) in the dimerization domain of bHLH2 prevents transcription of the two cytochrome P450 genes, resulting in the sweet kernel trait.

Original language	English
Journal	Science
Volume	364
Issue number	6445
Pages (from-to)	1095-1098
ISSN	0036-8075
DOIs	https://doi.org/10.1126/science.aav8197
Publication status	Published - 14 Jun 2019

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title = "Mutation of a bHLH transcription factor allowed almond domestication",

abstract = "Wild almond species accumulate the bitter and toxic cyanogenic diglucoside amygdalin. Almond domestication was enabled by the selection of genotypes harboring sweet kernels. We report the completion of the almond reference genome. Map-based cloning using an F1 population segregating for kernel taste led to the identification of a 46-kilobase gene cluster encoding five basic helix-loop-helix transcription factors, bHLH1 to bHLH5. Functional characterization demonstrated that bHLH2 controls transcription of the P450 monooxygenase–encoding genes PdCYP79D16 and PdCYP71AN24, which are involved in the amygdalin biosynthetic pathway. A nonsynonymous point mutation (Leu to Phe) in the dimerization domain of bHLH2 prevents transcription of the two cytochrome P450 genes, resulting in the sweet kernel trait.",

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year = "2019",

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doi = "10.1126/science.aav8197",

language = "English",

volume = "364",

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T1 - Mutation of a bHLH transcription factor allowed almond domestication

AU - Sánchez-Pérez, R.

AU - Pavan, S.

AU - Mazzeo, R.

AU - Moldovan, C.

AU - Aiese Cigliano, R.

AU - Del Cueto, J.

AU - Ricciardi, F.

AU - Lotti, C.

AU - Ricciardi, L.

AU - Dicenta, F.

AU - López-Marqués, R. L.

AU - Lindberg Møller, B.

PY - 2019/6/14

Y1 - 2019/6/14

N2 - Wild almond species accumulate the bitter and toxic cyanogenic diglucoside amygdalin. Almond domestication was enabled by the selection of genotypes harboring sweet kernels. We report the completion of the almond reference genome. Map-based cloning using an F1 population segregating for kernel taste led to the identification of a 46-kilobase gene cluster encoding five basic helix-loop-helix transcription factors, bHLH1 to bHLH5. Functional characterization demonstrated that bHLH2 controls transcription of the P450 monooxygenase–encoding genes PdCYP79D16 and PdCYP71AN24, which are involved in the amygdalin biosynthetic pathway. A nonsynonymous point mutation (Leu to Phe) in the dimerization domain of bHLH2 prevents transcription of the two cytochrome P450 genes, resulting in the sweet kernel trait.

AB - Wild almond species accumulate the bitter and toxic cyanogenic diglucoside amygdalin. Almond domestication was enabled by the selection of genotypes harboring sweet kernels. We report the completion of the almond reference genome. Map-based cloning using an F1 population segregating for kernel taste led to the identification of a 46-kilobase gene cluster encoding five basic helix-loop-helix transcription factors, bHLH1 to bHLH5. Functional characterization demonstrated that bHLH2 controls transcription of the P450 monooxygenase–encoding genes PdCYP79D16 and PdCYP71AN24, which are involved in the amygdalin biosynthetic pathway. A nonsynonymous point mutation (Leu to Phe) in the dimerization domain of bHLH2 prevents transcription of the two cytochrome P450 genes, resulting in the sweet kernel trait.

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DO - 10.1126/science.aav8197

M3 - Journal article

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AN - SCOPUS:85067619386

SN - 0036-8075

VL - 364

SP - 1095

EP - 1098

JO - Science

JF - Science

IS - 6445

ER -

Mutation of a bHLH transcription factor allowed almond domestication

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