Muscle interstitial potassium kinetics during intense exhaustive exercise: effect of previous arm exercise

TY - JOUR

T1 - Muscle interstitial potassium kinetics during intense exhaustive exercise

T2 - effect of previous arm exercise

AU - Nordsborg, Nikolai

AU - Mohr, Magni

AU - Pedersen, Lasse Dannemann

AU - Nielsen, Jens Jung

AU - Langberg, Henning

AU - Bangsbo, Jens

N1 - PUF 2003 5200 063

PY - 2003

Y1 - 2003

N2 - Interstitial K+ ([K+]i) was measured in human skeletal muscle by microdialysis during exhaustive leg exercise, with (AL) and without (L) previous intense arm exercise. In addition, the reproducibility of the [K+]i determinations was examined. Possible microdialysis-induced rupture of the sarcolemma was assessed by measurement of carnosine in the dialysate, because carnosine is only expected to be found intracellularly. Changes in [K+]i could be reproduced, when exhaustive leg exercise was performed on two different days, with a between-day difference of approximately 0.5 mM at rest and 1.5 mM at exhaustion. The time to exhaustion was shorter in AL than in L (2.7 +/- 0.3 vs. 4.0 +/- 0.3 min; P <0.05). Furthermore, [K+]i was higher from 0 to 1.5 min of the intense leg exercise period in AL compared with L (9.2 +/- 0.7 vs. 6.4 +/- 0.9 mM; P <0.001) and at exhaustion (11.9 +/- 0.5 vs. 10.3 +/- 0.6 mM; P <0.05). The dialysate content of carnosine was elevated by exercise, but low-intensity exercise resulted in higher dialysate carnosine concentrations than subsequent intense exercise. Furthermore, no relationship was found between carnosine concentrations and [K+]i. Thus the present data suggest that microdialysis can be used to determine muscle [K+]i kinetics during intense exercise, when low-intensity exercise is performed before the intense exercise. The high [K+]i levels reached at exhaustion can be expected to cause fatigue, which is supported by the finding that a faster accumulation of interstitial K+, induced by prior arm exercise, was associated with a reduced time to fatigue.

AB - Interstitial K+ ([K+]i) was measured in human skeletal muscle by microdialysis during exhaustive leg exercise, with (AL) and without (L) previous intense arm exercise. In addition, the reproducibility of the [K+]i determinations was examined. Possible microdialysis-induced rupture of the sarcolemma was assessed by measurement of carnosine in the dialysate, because carnosine is only expected to be found intracellularly. Changes in [K+]i could be reproduced, when exhaustive leg exercise was performed on two different days, with a between-day difference of approximately 0.5 mM at rest and 1.5 mM at exhaustion. The time to exhaustion was shorter in AL than in L (2.7 +/- 0.3 vs. 4.0 +/- 0.3 min; P <0.05). Furthermore, [K+]i was higher from 0 to 1.5 min of the intense leg exercise period in AL compared with L (9.2 +/- 0.7 vs. 6.4 +/- 0.9 mM; P <0.001) and at exhaustion (11.9 +/- 0.5 vs. 10.3 +/- 0.6 mM; P <0.05). The dialysate content of carnosine was elevated by exercise, but low-intensity exercise resulted in higher dialysate carnosine concentrations than subsequent intense exercise. Furthermore, no relationship was found between carnosine concentrations and [K+]i. Thus the present data suggest that microdialysis can be used to determine muscle [K+]i kinetics during intense exercise, when low-intensity exercise is performed before the intense exercise. The high [K+]i levels reached at exhaustion can be expected to cause fatigue, which is supported by the finding that a faster accumulation of interstitial K+, induced by prior arm exercise, was associated with a reduced time to fatigue.

KW - Acidosis

KW - Adult

KW - Arm

KW - Carnosine

KW - Extracellular Space

KW - Humans

KW - Hydrogen-Ion Concentration

KW - Kinetics

KW - Leg

KW - Male

KW - Microdialysis

KW - Muscle Fatigue

KW - Muscle, Skeletal

KW - Physical Endurance

KW - Potassium

U2 - 10.1152/ajpregu.00029.2003

DO - 10.1152/ajpregu.00029.2003

M3 - Journal article

C2 - 12663256

SN - 0363-6119

VL - 285

SP - R143-R148

JO - American Journal of Physiology - Regulatory Integrative and Comparative Physiology

JF - American Journal of Physiology - Regulatory Integrative and Comparative Physiology

IS - 1

ER -