Multilocus sequence typing of Enterococcus faecalis isolates demonstrating different lesion types in broiler breeders

TY - JOUR

T1 - Multilocus sequence typing of Enterococcus faecalis isolates demonstrating different lesion types in broiler breeders

AU - Olsen, Rikke Heidemann

AU - Petersen, A.

AU - Christensen, Henrik

AU - Bisgaard, Magne

PY - 2010/12

Y1 - 2010/12

N2 - A total of 69 isolates of Enterococcus faecalis from broiler breeders demonstrating different lesion types and representing eight different flocks were characterized by multilocus sequence typing. Twenty isolates obtained from healthy birds representing two additional flocks were included for comparison. A total of 12 different sequence types (STs) was obtained. Correlation between ST and lesion type was not demonstrated. However, three STs (ST82, ST174, ST177) made up 81% of the isolates associated with lesions, indicating that these STs might be particularly associated with birds. In addition, ST174, the most frequently demonstrated ST, was only obtained from affected birds. Surprisingly, ST82, previously reported to be associated with amyloid arthropathy in layers worldwide, demonstrated a high degree of diversity as to lesion types, just as healthy carriers were demonstrated among broiler breeders. STs associated with healthy birds and lesions, respectively, did not demonstrate a phylogenetic relationship.

AB - A total of 69 isolates of Enterococcus faecalis from broiler breeders demonstrating different lesion types and representing eight different flocks were characterized by multilocus sequence typing. Twenty isolates obtained from healthy birds representing two additional flocks were included for comparison. A total of 12 different sequence types (STs) was obtained. Correlation between ST and lesion type was not demonstrated. However, three STs (ST82, ST174, ST177) made up 81% of the isolates associated with lesions, indicating that these STs might be particularly associated with birds. In addition, ST174, the most frequently demonstrated ST, was only obtained from affected birds. Surprisingly, ST82, previously reported to be associated with amyloid arthropathy in layers worldwide, demonstrated a high degree of diversity as to lesion types, just as healthy carriers were demonstrated among broiler breeders. STs associated with healthy birds and lesions, respectively, did not demonstrate a phylogenetic relationship.

U2 - 10.1080/03079457.2010.517250

DO - 10.1080/03079457.2010.517250

M3 - Journal article

C2 - 21154051

SN - 0307-9457

VL - 39

SP - 435

EP - 440

JO - Avian Pathology

JF - Avian Pathology

IS - 6

ER -