TY - JOUR
T1 - Mouse B- and T-cell colony formation in vitro. I. Separation of colony-promoting and -inhibiting activities in concanavalin A rat spleen conditioned medium.
AU - Claësson, M H
AU - Nissen, Mogens Holst
AU - Röpke, C
N1 - Keywords: Animals; B-Lymphocytes; Cell Division; Chromatography, Gel; Colony-Stimulating Factors; Concanavalin A; Culture Media; Mice; Rats; Spleen; T-Lymphocytes; T-Lymphocytes, Regulatory
PY - 1984
Y1 - 1984
N2 - Rat spleen cell cultures exposed for 24 h to concanavalin A (Con A-CM) contain, in addition to interleukin 2 (IL-2), factors that promote colony formation in vitro by mouse T cells (TCPA) and B cells (BCPA). TCPA and BCPA are separable on a Sephadex G-75 column. TCPA has a molecular weight of 15,000 daltons and shows the same elution profile as IL-2. Absorption studies with Con A-activated T cells suggested that TCPA and IL-2 are the same entity. BCPA has an apparent molecular weight of 45,000 daltons and stimulates colony formation by B lymphocytes seeded at very low cell density (10(4) - 5 X 10(4) cells/ml). In contrast to TCPA, BCPA can only be demonstrated in gel-filtered material owing to the presence of B colony suppressor activities in crude Con A-CM. Two B colony inhibitory activities were demonstrated by AcA 34 chromatography of crude Con A-CM with molecular weights of 80,000-130,000 and about 50,000, respectively. Because of the specificity, simplicity and sensitivity of B and T colony formation these assay systems should be valuable tools for in vitro testing of biological activities regulating the immune system.
AB - Rat spleen cell cultures exposed for 24 h to concanavalin A (Con A-CM) contain, in addition to interleukin 2 (IL-2), factors that promote colony formation in vitro by mouse T cells (TCPA) and B cells (BCPA). TCPA and BCPA are separable on a Sephadex G-75 column. TCPA has a molecular weight of 15,000 daltons and shows the same elution profile as IL-2. Absorption studies with Con A-activated T cells suggested that TCPA and IL-2 are the same entity. BCPA has an apparent molecular weight of 45,000 daltons and stimulates colony formation by B lymphocytes seeded at very low cell density (10(4) - 5 X 10(4) cells/ml). In contrast to TCPA, BCPA can only be demonstrated in gel-filtered material owing to the presence of B colony suppressor activities in crude Con A-CM. Two B colony inhibitory activities were demonstrated by AcA 34 chromatography of crude Con A-CM with molecular weights of 80,000-130,000 and about 50,000, respectively. Because of the specificity, simplicity and sensitivity of B and T colony formation these assay systems should be valuable tools for in vitro testing of biological activities regulating the immune system.
M3 - Journal article
C2 - 6231716
SN - 0301-6323
VL - 19
SP - 205
EP - 210
JO - Scandinavian Journal of Immunology, Supplement
JF - Scandinavian Journal of Immunology, Supplement
IS - 3
ER -