Morphometric, molecular and histopathologic description of hepatic infection by Orthosplanchnus arcticus (Trematoda: Digenea: Brachycladiidae) in ringed seals (Pusa hispida) from Northwest Greenland

Emilie Andersen-Ranberg; Kristina Lehnert; Páll S. Leifsson; Rune Dietz; Steen Andersen; Ursula Siebert; Lena Measures; Christian Sonne

doi:10.1007/s00300-017-2245-6

Morphometric, molecular and histopathologic description of hepatic infection by Orthosplanchnus arcticus (Trematoda: Digenea: Brachycladiidae) in ringed seals (Pusa hispida) from Northwest Greenland

Emilie Andersen-Ranberg^*, Kristina Lehnert, Páll S. Leifsson, Rune Dietz, Steen Andersen, Ursula Siebert, Lena Measures, Christian Sonne

^*Corresponding author for this work

1 Citation (Scopus)

Abstract

For the first time in > 30 years of routine sampling under the Arctic Monitoring and Assessment Program, a parasite was found in the liver of ringed seals (Phoca hispida) collected near Qaanaaq (Thule), Northwest Greenland, in 2008 and 2014. Concerns regarding changes to parasite occurrence, possibly related to climate change and bioaccumulation of immunomodulating anthropogenic pollutants, spurred further investigations into parasite characterization, and implications for wildlife health and seal hunters. Microscopic, molecular, and morphometric analyses are presented herein. Of 40 seals, 6 (15%) were infected, and 5 of 6 of these seals had severe infections. The parasite was identified morphologically as Orthosplanchnus arcticus Odhner, 1905 (Trematoda; Digenea: Brachycladiidae). Macro- and microscopic pathologic study indicated mild-to-severe biliary hyperplasia associated, stasis associated, and fibrosis associated with trematode infections. Molecular analysis of the trematode confirmed its classification within the Brachycladiidae using sequencing and comparing Internal Transcribed Spacer-1, mitochondrial-NADH Dehydrogenase 3, 18S ribosomal DNA, and Cyclooxygenase-1 regions. This is the first ever report of O. arcticus in ringed seals from Qaanaaq and is one of the most northern records of this parasite. We found significant liver pathology in severely infected seals, but its effects on health of seals in this population are unknown. Host-specificity and the lifecycle of O. arcticus are unknown, but transmission may involve subsistence and commercially harvested Arctic fish species. Further work is needed to answer these questions. Surveying parasites in Arctic wildlife is important in order to assess potential effects on wildlife and human health (i.e., zoonoses).

Original language	English
Journal	Polar Biology
Volume	41
Issue number	5
Pages (from-to)	1019-1025
ISSN	0722-4060
DOIs	https://doi.org/10.1007/s00300-017-2245-6
Publication status	Published - 1 May 2018

Keywords

Molecular identification
Morphometrics
MT-ND3
PCR
Pinniped
Trematoda

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/s00300-017-2245-6

Cite this

Andersen-Ranberg, E., Lehnert, K., Leifsson, P. S., Dietz, R., Andersen, S., Siebert, U., Measures, L., & Sonne, C. (2018). Morphometric, molecular and histopathologic description of hepatic infection by Orthosplanchnus arcticus (Trematoda: Digenea: Brachycladiidae) in ringed seals (Pusa hispida) from Northwest Greenland. Polar Biology, 41(5), 1019-1025. https://doi.org/10.1007/s00300-017-2245-6

Morphometric, molecular and histopathologic description of hepatic infection by Orthosplanchnus arcticus (Trematoda: Digenea: Brachycladiidae) in ringed seals (Pusa hispida) from Northwest Greenland. / Andersen-Ranberg, Emilie; Lehnert, Kristina; Leifsson, Páll S. et al.

In: Polar Biology, Vol. 41, No. 5, 01.05.2018, p. 1019-1025.

Research output: Contribution to journal › Journal article › Research › peer-review

Andersen-Ranberg, E, Lehnert, K, Leifsson, PS, Dietz, R, Andersen, S, Siebert, U, Measures, L & Sonne, C 2018, 'Morphometric, molecular and histopathologic description of hepatic infection by Orthosplanchnus arcticus (Trematoda: Digenea: Brachycladiidae) in ringed seals (Pusa hispida) from Northwest Greenland', Polar Biology, vol. 41, no. 5, pp. 1019-1025. https://doi.org/10.1007/s00300-017-2245-6

@article{acf83f8081c24101a6bacbee66a0766b,

title = "Morphometric, molecular and histopathologic description of hepatic infection by Orthosplanchnus arcticus (Trematoda: Digenea: Brachycladiidae) in ringed seals (Pusa hispida) from Northwest Greenland",

abstract = "For the first time in > 30 years of routine sampling under the Arctic Monitoring and Assessment Program, a parasite was found in the liver of ringed seals (Phoca hispida) collected near Qaanaaq (Thule), Northwest Greenland, in 2008 and 2014. Concerns regarding changes to parasite occurrence, possibly related to climate change and bioaccumulation of immunomodulating anthropogenic pollutants, spurred further investigations into parasite characterization, and implications for wildlife health and seal hunters. Microscopic, molecular, and morphometric analyses are presented herein. Of 40 seals, 6 (15%) were infected, and 5 of 6 of these seals had severe infections. The parasite was identified morphologically as Orthosplanchnus arcticus Odhner, 1905 (Trematoda; Digenea: Brachycladiidae). Macro- and microscopic pathologic study indicated mild-to-severe biliary hyperplasia associated, stasis associated, and fibrosis associated with trematode infections. Molecular analysis of the trematode confirmed its classification within the Brachycladiidae using sequencing and comparing Internal Transcribed Spacer-1, mitochondrial-NADH Dehydrogenase 3, 18S ribosomal DNA, and Cyclooxygenase-1 regions. This is the first ever report of O. arcticus in ringed seals from Qaanaaq and is one of the most northern records of this parasite. We found significant liver pathology in severely infected seals, but its effects on health of seals in this population are unknown. Host-specificity and the lifecycle of O. arcticus are unknown, but transmission may involve subsistence and commercially harvested Arctic fish species. Further work is needed to answer these questions. Surveying parasites in Arctic wildlife is important in order to assess potential effects on wildlife and human health (i.e., zoonoses).",

keywords = "Molecular identification, Morphometrics, MT-ND3, PCR, Pinniped, Trematoda",

author = "Emilie Andersen-Ranberg and Kristina Lehnert and Leifsson, {P{\'a}ll S.} and Rune Dietz and Steen Andersen and Ursula Siebert and Lena Measures and Christian Sonne",

year = "2018",

month = may,

day = "1",

doi = "10.1007/s00300-017-2245-6",

language = "English",

volume = "41",

pages = "1019--1025",

journal = "Polar Biology",

issn = "0722-4060",

publisher = "Springer",

number = "5",

}

TY - JOUR

T1 - Morphometric, molecular and histopathologic description of hepatic infection by Orthosplanchnus arcticus (Trematoda: Digenea: Brachycladiidae) in ringed seals (Pusa hispida) from Northwest Greenland

AU - Andersen-Ranberg, Emilie

AU - Lehnert, Kristina

AU - Leifsson, Páll S.

AU - Dietz, Rune

AU - Andersen, Steen

AU - Siebert, Ursula

AU - Measures, Lena

AU - Sonne, Christian

PY - 2018/5/1

Y1 - 2018/5/1

N2 - For the first time in > 30 years of routine sampling under the Arctic Monitoring and Assessment Program, a parasite was found in the liver of ringed seals (Phoca hispida) collected near Qaanaaq (Thule), Northwest Greenland, in 2008 and 2014. Concerns regarding changes to parasite occurrence, possibly related to climate change and bioaccumulation of immunomodulating anthropogenic pollutants, spurred further investigations into parasite characterization, and implications for wildlife health and seal hunters. Microscopic, molecular, and morphometric analyses are presented herein. Of 40 seals, 6 (15%) were infected, and 5 of 6 of these seals had severe infections. The parasite was identified morphologically as Orthosplanchnus arcticus Odhner, 1905 (Trematoda; Digenea: Brachycladiidae). Macro- and microscopic pathologic study indicated mild-to-severe biliary hyperplasia associated, stasis associated, and fibrosis associated with trematode infections. Molecular analysis of the trematode confirmed its classification within the Brachycladiidae using sequencing and comparing Internal Transcribed Spacer-1, mitochondrial-NADH Dehydrogenase 3, 18S ribosomal DNA, and Cyclooxygenase-1 regions. This is the first ever report of O. arcticus in ringed seals from Qaanaaq and is one of the most northern records of this parasite. We found significant liver pathology in severely infected seals, but its effects on health of seals in this population are unknown. Host-specificity and the lifecycle of O. arcticus are unknown, but transmission may involve subsistence and commercially harvested Arctic fish species. Further work is needed to answer these questions. Surveying parasites in Arctic wildlife is important in order to assess potential effects on wildlife and human health (i.e., zoonoses).

AB - For the first time in > 30 years of routine sampling under the Arctic Monitoring and Assessment Program, a parasite was found in the liver of ringed seals (Phoca hispida) collected near Qaanaaq (Thule), Northwest Greenland, in 2008 and 2014. Concerns regarding changes to parasite occurrence, possibly related to climate change and bioaccumulation of immunomodulating anthropogenic pollutants, spurred further investigations into parasite characterization, and implications for wildlife health and seal hunters. Microscopic, molecular, and morphometric analyses are presented herein. Of 40 seals, 6 (15%) were infected, and 5 of 6 of these seals had severe infections. The parasite was identified morphologically as Orthosplanchnus arcticus Odhner, 1905 (Trematoda; Digenea: Brachycladiidae). Macro- and microscopic pathologic study indicated mild-to-severe biliary hyperplasia associated, stasis associated, and fibrosis associated with trematode infections. Molecular analysis of the trematode confirmed its classification within the Brachycladiidae using sequencing and comparing Internal Transcribed Spacer-1, mitochondrial-NADH Dehydrogenase 3, 18S ribosomal DNA, and Cyclooxygenase-1 regions. This is the first ever report of O. arcticus in ringed seals from Qaanaaq and is one of the most northern records of this parasite. We found significant liver pathology in severely infected seals, but its effects on health of seals in this population are unknown. Host-specificity and the lifecycle of O. arcticus are unknown, but transmission may involve subsistence and commercially harvested Arctic fish species. Further work is needed to answer these questions. Surveying parasites in Arctic wildlife is important in order to assess potential effects on wildlife and human health (i.e., zoonoses).

KW - Molecular identification

KW - Morphometrics

KW - MT-ND3

KW - PCR

KW - Pinniped

KW - Trematoda

U2 - 10.1007/s00300-017-2245-6

DO - 10.1007/s00300-017-2245-6

M3 - Journal article

AN - SCOPUS:85040648699

SN - 0722-4060

VL - 41

SP - 1019

EP - 1025

JO - Polar Biology

JF - Polar Biology

IS - 5

ER -

Morphometric, molecular and histopathologic description of hepatic infection by Orthosplanchnus arcticus (Trematoda: Digenea: Brachycladiidae) in ringed seals (Pusa hispida) from Northwest Greenland

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Cite this