Morphology and Structure of Solid Lipid Nanoparticles Loaded with High Concentrations of β-Carotene

TY - JOUR

T1 - Morphology and Structure of Solid Lipid Nanoparticles Loaded with High Concentrations of β-Carotene

AU - Schjoerring-Thyssen, Jakob

AU - Olsen, Karsten

AU - Koehler, Klaus

AU - Jouenne, Eric

AU - Rousseau, Dérick

AU - Andersen, Mogens Larsen

PY - 2019/11/6

Y1 - 2019/11/6

N2 - Solid lipid nanoparticles (SLNs) containing up to 37.5 wt % all-trans β-carotene in the lipid phase are potential water-dispersible food colorants. SLNs have been made by hot-melt high-pressure homogenization with fully hydrogenated sunflower oil and with polysorbate 80 and sunflower lecithin as stabilizers. Atomic force microscopy revealed the SLNs had thin platelet structures most likely derived from the triglyceride crystal β-form, as detected by X-ray diffraction. No indications of crystalline β-carotene were detected. High-performance liquid chromatography analysis showed the extensive isomerization of β-carotene into more than 10 cis isomers, suggesting that it is present as an amorphous mixture. The high β-carotene loadings did not affect the triglyceride crystal structure and the morphology of the SLNs. It is suggested the SLNs consist of a platelet core of crystalline triglyceride surrounded by an amorphous β-carotene-containing layer. The layered structure is suggested to affect the coloring power of the SLNs at β-carotene loadings above 15 wt % of the lipid phase.

AB - Solid lipid nanoparticles (SLNs) containing up to 37.5 wt % all-trans β-carotene in the lipid phase are potential water-dispersible food colorants. SLNs have been made by hot-melt high-pressure homogenization with fully hydrogenated sunflower oil and with polysorbate 80 and sunflower lecithin as stabilizers. Atomic force microscopy revealed the SLNs had thin platelet structures most likely derived from the triglyceride crystal β-form, as detected by X-ray diffraction. No indications of crystalline β-carotene were detected. High-performance liquid chromatography analysis showed the extensive isomerization of β-carotene into more than 10 cis isomers, suggesting that it is present as an amorphous mixture. The high β-carotene loadings did not affect the triglyceride crystal structure and the morphology of the SLNs. It is suggested the SLNs consist of a platelet core of crystalline triglyceride surrounded by an amorphous β-carotene-containing layer. The layered structure is suggested to affect the coloring power of the SLNs at β-carotene loadings above 15 wt % of the lipid phase.

KW - AFM

KW - food color

KW - SLN

KW - X-ray diffraction

KW - β-carotene

U2 - 10.1021/acs.jafc.9b04215

DO - 10.1021/acs.jafc.9b04215

M3 - Journal article

C2 - 31610122

AN - SCOPUS:85074224882

SN - 0021-8561

VL - 67

SP - 12273

EP - 12282

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 44

ER -