Monitoring variables affecting positron emission tomography measurements of cerebral blood flow in anaesthetized pigs

Aage Kristian Olsen Alstrup; Nora Elisabeth Zois; Mette Simonsen; Ole Lajord Munk

doi:10.1186/s13028-018-0369-5

Monitoring variables affecting positron emission tomography measurements of cerebral blood flow in anaesthetized pigs

Aage Kristian Olsen Alstrup^*, Nora Elisabeth Zois, Mette Simonsen, Ole Lajord Munk

^*Corresponding author for this work

1 Citation (Scopus)

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Abstract

Background: Positron emission tomography (PET) imaging of anaesthetized pig brains is a useful tool in neuroscience. Stable cerebral blood flow (CBF) is essential for PET, since variations can affect the distribution of several radiotracers. However, the effect of physiological factors regulating CBF is unresolved and therefore knowledge of optimal anaesthesia and monitoring of pigs in PET studies is sparse. The aim of this study was therefore to determine if and how physiological variables and the duration of anaesthesia affected CBF as measured by PET using (¹⁵O)-water in isoflurane-N₂O anaesthetized domestic female pigs. First, we examined how physiological monitoring parameters were associated with CBF, and which parameters should be monitored and if possible kept constant, during studies where a stable CBF is important. Secondly, we examined how the duration of anaesthesia affected CBF and the monitoring parameters. Results: No significant statistical correlations were found between CBF and the nine monitoring variables. However, we found that arterial carbon dioxide tension (PaCO₂) and body temperature were important predictors of CBF that should be observed and kept constant. In addition, we found that long-duration anaesthesia was significantly correlated with high heart rate, low arterial oxygen tension, and high body temperature, but not with CBF. Conclusions: The findings indicate that PaCO₂ and body temperature are crucial for maintaining stable levels of CBF and thus optimizing PET imaging of molecular mechanisms in the brain of anaesthetized pigs. Therefore, as a minimum these two variables should be monitored and kept constant. Furthermore, the duration of anaesthesia should be kept constant to avoid variations in monitoring variables.

Original language	English
Article number	17
Journal	Acta Veterinaria Scandinavica
Volume	60
Number of pages	7
ISSN	0044-605X
DOIs	https://doi.org/10.1186/s13028-018-0369-5
Publication status	Published - 2018

Keywords

(O)-water
Animal
Brain research
CBF
Positron emission tomography
Swine

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@article{10334b6b2dc9412ab5dca6819922b722,

title = "Monitoring variables affecting positron emission tomography measurements of cerebral blood flow in anaesthetized pigs",

abstract = "Background: Positron emission tomography (PET) imaging of anaesthetized pig brains is a useful tool in neuroscience. Stable cerebral blood flow (CBF) is essential for PET, since variations can affect the distribution of several radiotracers. However, the effect of physiological factors regulating CBF is unresolved and therefore knowledge of optimal anaesthesia and monitoring of pigs in PET studies is sparse. The aim of this study was therefore to determine if and how physiological variables and the duration of anaesthesia affected CBF as measured by PET using (15O)-water in isoflurane-N2O anaesthetized domestic female pigs. First, we examined how physiological monitoring parameters were associated with CBF, and which parameters should be monitored and if possible kept constant, during studies where a stable CBF is important. Secondly, we examined how the duration of anaesthesia affected CBF and the monitoring parameters. Results: No significant statistical correlations were found between CBF and the nine monitoring variables. However, we found that arterial carbon dioxide tension (PaCO2) and body temperature were important predictors of CBF that should be observed and kept constant. In addition, we found that long-duration anaesthesia was significantly correlated with high heart rate, low arterial oxygen tension, and high body temperature, but not with CBF. Conclusions: The findings indicate that PaCO2 and body temperature are crucial for maintaining stable levels of CBF and thus optimizing PET imaging of molecular mechanisms in the brain of anaesthetized pigs. Therefore, as a minimum these two variables should be monitored and kept constant. Furthermore, the duration of anaesthesia should be kept constant to avoid variations in monitoring variables.",

keywords = "(O)-water, Animal, Brain research, CBF, Positron emission tomography, Swine",

author = "Alstrup, {Aage Kristian Olsen} and Zois, {Nora Elisabeth} and Mette Simonsen and Munk, {Ole Lajord}",

year = "2018",

doi = "10.1186/s13028-018-0369-5",

language = "English",

volume = "60",

journal = "Acta Veterinaria Scandinavica",

issn = "0044-605X",

publisher = "BioMed Central Ltd.",

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TY - JOUR

T1 - Monitoring variables affecting positron emission tomography measurements of cerebral blood flow in anaesthetized pigs

AU - Alstrup, Aage Kristian Olsen

AU - Zois, Nora Elisabeth

AU - Simonsen, Mette

AU - Munk, Ole Lajord

PY - 2018

Y1 - 2018

N2 - Background: Positron emission tomography (PET) imaging of anaesthetized pig brains is a useful tool in neuroscience. Stable cerebral blood flow (CBF) is essential for PET, since variations can affect the distribution of several radiotracers. However, the effect of physiological factors regulating CBF is unresolved and therefore knowledge of optimal anaesthesia and monitoring of pigs in PET studies is sparse. The aim of this study was therefore to determine if and how physiological variables and the duration of anaesthesia affected CBF as measured by PET using (15O)-water in isoflurane-N2O anaesthetized domestic female pigs. First, we examined how physiological monitoring parameters were associated with CBF, and which parameters should be monitored and if possible kept constant, during studies where a stable CBF is important. Secondly, we examined how the duration of anaesthesia affected CBF and the monitoring parameters. Results: No significant statistical correlations were found between CBF and the nine monitoring variables. However, we found that arterial carbon dioxide tension (PaCO2) and body temperature were important predictors of CBF that should be observed and kept constant. In addition, we found that long-duration anaesthesia was significantly correlated with high heart rate, low arterial oxygen tension, and high body temperature, but not with CBF. Conclusions: The findings indicate that PaCO2 and body temperature are crucial for maintaining stable levels of CBF and thus optimizing PET imaging of molecular mechanisms in the brain of anaesthetized pigs. Therefore, as a minimum these two variables should be monitored and kept constant. Furthermore, the duration of anaesthesia should be kept constant to avoid variations in monitoring variables.

AB - Background: Positron emission tomography (PET) imaging of anaesthetized pig brains is a useful tool in neuroscience. Stable cerebral blood flow (CBF) is essential for PET, since variations can affect the distribution of several radiotracers. However, the effect of physiological factors regulating CBF is unresolved and therefore knowledge of optimal anaesthesia and monitoring of pigs in PET studies is sparse. The aim of this study was therefore to determine if and how physiological variables and the duration of anaesthesia affected CBF as measured by PET using (15O)-water in isoflurane-N2O anaesthetized domestic female pigs. First, we examined how physiological monitoring parameters were associated with CBF, and which parameters should be monitored and if possible kept constant, during studies where a stable CBF is important. Secondly, we examined how the duration of anaesthesia affected CBF and the monitoring parameters. Results: No significant statistical correlations were found between CBF and the nine monitoring variables. However, we found that arterial carbon dioxide tension (PaCO2) and body temperature were important predictors of CBF that should be observed and kept constant. In addition, we found that long-duration anaesthesia was significantly correlated with high heart rate, low arterial oxygen tension, and high body temperature, but not with CBF. Conclusions: The findings indicate that PaCO2 and body temperature are crucial for maintaining stable levels of CBF and thus optimizing PET imaging of molecular mechanisms in the brain of anaesthetized pigs. Therefore, as a minimum these two variables should be monitored and kept constant. Furthermore, the duration of anaesthesia should be kept constant to avoid variations in monitoring variables.

KW - (O)-water

KW - Animal

KW - Brain research

KW - CBF

KW - Positron emission tomography

KW - Swine

U2 - 10.1186/s13028-018-0369-5

DO - 10.1186/s13028-018-0369-5

M3 - Journal article

C2 - 29530059

AN - SCOPUS:85043477094

SN - 0044-605X

VL - 60

JO - Acta Veterinaria Scandinavica

JF - Acta Veterinaria Scandinavica

M1 - 17

ER -

Monitoring variables affecting positron emission tomography measurements of cerebral blood flow in anaesthetized pigs

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