Molecular surveillance of drug resistance through imported isolates of Plasmodium falciparum in Europe

Tomas Jelinek; Gabriele Peyerl-Hoffmann; Nikolai Mühlberger; Ole Wichmann; Michael Wilhelm; Nadja Schmider; Martin P Grobusch; Frank von Sonnenburg; Joaquim Gascon; Hermann Laferl; Christoph Hatz; Michael Alifrangis; Gerd Burchard; Paul McWhinney; Marco Schulze; Herwig Kollaritsch; Saraiva da Cunha; Jiri Beran; Peter Kern; Ida Gjørup; Juan Cuadros

Molecular surveillance of drug resistance through imported isolates of Plasmodium falciparum in Europe

Tomas Jelinek, Gabriele Peyerl-Hoffmann, Nikolai Mühlberger, Ole Wichmann, Michael Wilhelm, Nadja Schmider, Martin P Grobusch, Frank von Sonnenburg, Joaquim Gascon, Hermann Laferl, Christoph Hatz, Michael Alifrangis, Gerd Burchard, Paul McWhinney, Marco Schulze, Herwig Kollaritsch, Saraiva da Cunha, Jiri Beran, Peter Kern, Ida GjørupJuan Cuadros

21 Citations (Scopus)

Abstract

BACKGROUND: Results from numerous studies point convincingly to correlations between mutations at selected genes and phenotypic resistance to antimalarials in Plasmodium falciparum isolates. In order to move molecular assays for point mutations on resistance-related genes into the realm of applied tools for surveillance, we investigated a selection of P. falciparum isolates that were imported during the year 2001 into Europe to study the prevalence of resistance-associated point mutations at relevant codons. In particular, we tested for parasites which were developing resistance to antifolates and chloroquine. The screening results were used to map the prevalence of mutations and, thus, levels of potential drug resistance in endemic areas world-wide. RESULTS: 337 isolates have been tested so far. Prevalence of mutations that are associated with resistance to chloroquine on the pfcrt and pfmdr genes of P. falciparum was demonstrated at high levels. However, the prevalence of mutations associated with resistance to antifolates at the DHFR and DHPS genes was unexpectedly low, rarely exceeding 60% in endemic areas. CONCLUSIONS: Constant screening of imported isolates will enable TropNetEurop to establish a screening tool for emerging resistance in endemic areas.

Original language	English
Journal	Malaria Journal
Volume	1
Pages (from-to)	11
ISSN	1475-2875
Publication status	Published - 2002

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Jelinek, T., Peyerl-Hoffmann, G., Mühlberger, N., Wichmann, O., Wilhelm, M., Schmider, N., Grobusch, M. P., von Sonnenburg, F., Gascon, J., Laferl, H., Hatz, C., Alifrangis, M., Burchard, G., McWhinney, P., Schulze, M., Kollaritsch, H., da Cunha, S., Beran, J., Kern, P., ... Cuadros, J. (2002). Molecular surveillance of drug resistance through imported isolates of Plasmodium falciparum in Europe. Malaria Journal, 1, 11.

Jelinek, T, Peyerl-Hoffmann, G, Mühlberger, N, Wichmann, O, Wilhelm, M, Schmider, N, Grobusch, MP, von Sonnenburg, F, Gascon, J, Laferl, H, Hatz, C, Alifrangis, M, Burchard, G, McWhinney, P, Schulze, M, Kollaritsch, H, da Cunha, S, Beran, J, Kern, P, Gjørup, I & Cuadros, J 2002, 'Molecular surveillance of drug resistance through imported isolates of Plasmodium falciparum in Europe', Malaria Journal, vol. 1, pp. 11.

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title = "Molecular surveillance of drug resistance through imported isolates of Plasmodium falciparum in Europe",

abstract = "BACKGROUND: Results from numerous studies point convincingly to correlations between mutations at selected genes and phenotypic resistance to antimalarials in Plasmodium falciparum isolates. In order to move molecular assays for point mutations on resistance-related genes into the realm of applied tools for surveillance, we investigated a selection of P. falciparum isolates that were imported during the year 2001 into Europe to study the prevalence of resistance-associated point mutations at relevant codons. In particular, we tested for parasites which were developing resistance to antifolates and chloroquine. The screening results were used to map the prevalence of mutations and, thus, levels of potential drug resistance in endemic areas world-wide. RESULTS: 337 isolates have been tested so far. Prevalence of mutations that are associated with resistance to chloroquine on the pfcrt and pfmdr genes of P. falciparum was demonstrated at high levels. However, the prevalence of mutations associated with resistance to antifolates at the DHFR and DHPS genes was unexpectedly low, rarely exceeding 60% in endemic areas. CONCLUSIONS: Constant screening of imported isolates will enable TropNetEurop to establish a screening tool for emerging resistance in endemic areas.",

author = "Tomas Jelinek and Gabriele Peyerl-Hoffmann and Nikolai M{\"u}hlberger and Ole Wichmann and Michael Wilhelm and Nadja Schmider and Grobusch, {Martin P} and {von Sonnenburg}, Frank and Joaquim Gascon and Hermann Laferl and Christoph Hatz and Michael Alifrangis and Gerd Burchard and Paul McWhinney and Marco Schulze and Herwig Kollaritsch and {da Cunha}, Saraiva and Jiri Beran and Peter Kern and Ida Gj{\o}rup and Juan Cuadros",

note = "Keywords: ATP-Binding Cassette Transporters; Animals; Antimalarials; Chloroquine; Codon; Dihydropteroate Synthase; Drug Resistance; Drug Resistance, Multiple; Europe; Folic Acid Antagonists; Humans; Malaria, Falciparum; Membrane Proteins; Membrane Transport Proteins; Plasmodium falciparum; Point Mutation; Protozoan Proteins; Tetrahydrofolate Dehydrogenase; Travel",

year = "2002",

language = "English",

volume = "1",

pages = "11",

journal = "Malaria Journal",

issn = "1475-2875",

publisher = "BioMed Central",

}

TY - JOUR

T1 - Molecular surveillance of drug resistance through imported isolates of Plasmodium falciparum in Europe

AU - Jelinek, Tomas

AU - Peyerl-Hoffmann, Gabriele

AU - Mühlberger, Nikolai

AU - Wichmann, Ole

AU - Wilhelm, Michael

AU - Schmider, Nadja

AU - Grobusch, Martin P

AU - von Sonnenburg, Frank

AU - Gascon, Joaquim

AU - Laferl, Hermann

AU - Hatz, Christoph

AU - Alifrangis, Michael

AU - Burchard, Gerd

AU - McWhinney, Paul

AU - Schulze, Marco

AU - Kollaritsch, Herwig

AU - da Cunha, Saraiva

AU - Beran, Jiri

AU - Kern, Peter

AU - Gjørup, Ida

AU - Cuadros, Juan

N1 - Keywords: ATP-Binding Cassette Transporters; Animals; Antimalarials; Chloroquine; Codon; Dihydropteroate Synthase; Drug Resistance; Drug Resistance, Multiple; Europe; Folic Acid Antagonists; Humans; Malaria, Falciparum; Membrane Proteins; Membrane Transport Proteins; Plasmodium falciparum; Point Mutation; Protozoan Proteins; Tetrahydrofolate Dehydrogenase; Travel

PY - 2002

Y1 - 2002

N2 - BACKGROUND: Results from numerous studies point convincingly to correlations between mutations at selected genes and phenotypic resistance to antimalarials in Plasmodium falciparum isolates. In order to move molecular assays for point mutations on resistance-related genes into the realm of applied tools for surveillance, we investigated a selection of P. falciparum isolates that were imported during the year 2001 into Europe to study the prevalence of resistance-associated point mutations at relevant codons. In particular, we tested for parasites which were developing resistance to antifolates and chloroquine. The screening results were used to map the prevalence of mutations and, thus, levels of potential drug resistance in endemic areas world-wide. RESULTS: 337 isolates have been tested so far. Prevalence of mutations that are associated with resistance to chloroquine on the pfcrt and pfmdr genes of P. falciparum was demonstrated at high levels. However, the prevalence of mutations associated with resistance to antifolates at the DHFR and DHPS genes was unexpectedly low, rarely exceeding 60% in endemic areas. CONCLUSIONS: Constant screening of imported isolates will enable TropNetEurop to establish a screening tool for emerging resistance in endemic areas.

AB - BACKGROUND: Results from numerous studies point convincingly to correlations between mutations at selected genes and phenotypic resistance to antimalarials in Plasmodium falciparum isolates. In order to move molecular assays for point mutations on resistance-related genes into the realm of applied tools for surveillance, we investigated a selection of P. falciparum isolates that were imported during the year 2001 into Europe to study the prevalence of resistance-associated point mutations at relevant codons. In particular, we tested for parasites which were developing resistance to antifolates and chloroquine. The screening results were used to map the prevalence of mutations and, thus, levels of potential drug resistance in endemic areas world-wide. RESULTS: 337 isolates have been tested so far. Prevalence of mutations that are associated with resistance to chloroquine on the pfcrt and pfmdr genes of P. falciparum was demonstrated at high levels. However, the prevalence of mutations associated with resistance to antifolates at the DHFR and DHPS genes was unexpectedly low, rarely exceeding 60% in endemic areas. CONCLUSIONS: Constant screening of imported isolates will enable TropNetEurop to establish a screening tool for emerging resistance in endemic areas.

M3 - Journal article

C2 - 12423552

SN - 1475-2875

VL - 1

SP - 11

JO - Malaria Journal

JF - Malaria Journal

ER -

Molecular surveillance of drug resistance through imported isolates of Plasmodium falciparum in Europe

Abstract

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