Molecular evidence for sustained transmission of zoonotic Ascaris suum among zoo chimpanzees (Pan troglodytes)

TY - JOUR

T1 - Molecular evidence for sustained transmission of zoonotic Ascaris suum among zoo chimpanzees (Pan troglodytes)

AU - Nejsum, Peter

AU - Bertelsen, Mads Frost

AU - Betson, Martha

AU - Stothard, J. Russel

AU - Murrell, Kenneth Darwin

PY - 2010/8

Y1 - 2010/8

N2 - Chimpanzees in the Copenhagen Zoo frequently excrete ascarid worms onto the cage floor in spite of a regular anthelmintic treatment program. Previously it had been shown that the source of the infections was of pig origin. However, it was unknown whether the recurrence of the infection was due to reintroduction of eggs from an external source or to a sustained transmission cycle within the zoo. We found that isolated eggs were able to embryonate to the infective J3 stage and PCR-RFLP analysis on the ITS region amplified from single embryonated eggs suggest these to be Ascaris suum. In addition, sequence analysis of the cox1 gene ('barcoding') on expelled worms followed by cluster analysis revealed that the chimpanzees are infected with pig A. suum which now, in spite of control efforts, has stabilized into a permanent transmission cycle in the zoo's chimpanzee troop.

AB - Chimpanzees in the Copenhagen Zoo frequently excrete ascarid worms onto the cage floor in spite of a regular anthelmintic treatment program. Previously it had been shown that the source of the infections was of pig origin. However, it was unknown whether the recurrence of the infection was due to reintroduction of eggs from an external source or to a sustained transmission cycle within the zoo. We found that isolated eggs were able to embryonate to the infective J3 stage and PCR-RFLP analysis on the ITS region amplified from single embryonated eggs suggest these to be Ascaris suum. In addition, sequence analysis of the cox1 gene ('barcoding') on expelled worms followed by cluster analysis revealed that the chimpanzees are infected with pig A. suum which now, in spite of control efforts, has stabilized into a permanent transmission cycle in the zoo's chimpanzee troop.

KW - Former LIFE faculty

KW - Ascaris

KW - Zoonoses

KW - Chimpanzees

KW - Pigs

KW - Zoo

KW - Persistent transmission

U2 - 10.1016/j.vetpar.2010.03.030

DO - 10.1016/j.vetpar.2010.03.030

M3 - Journal article

C2 - 20400228

SN - 0304-4017

VL - 171

SP - 273

EP - 276

JO - Veterinary Parasitology

JF - Veterinary Parasitology

IS - 3-4

ER -