Molecular composition of IMP1 ribonucleoprotein granules.

Lars Jønson; Jonas Vikesaa; Anders Krogh; Lars K Nielsen; Thomas vO Hansen; Rehannah Borup; Anders H Johnsen; Jan Christiansen; Finn C Nielsen

doi:10.1074/mcp.M600346-MCP200

Molecular composition of IMP1 ribonucleoprotein granules.

Lars Jønson, Jonas Vikesaa, Anders Krogh, Lars K Nielsen, Thomas vO Hansen, Rehannah Borup, Anders H Johnsen, Jan Christiansen, Finn C Nielsen

150 Citations (Scopus)

Abstract

Localized mRNAs are transported to sites of local protein synthesis in large ribonucleoprotein (RNP) granules, but their molecular composition is incompletely understood. Insulin-like growth factor II mRNA-binding protein (IMP) zip code-binding proteins participate in mRNA localization, and in motile cells IMP-containing granules are dispersed around the nucleus and in cellular protrusions. We isolated the IMP1-containing RNP granules and found that they represent a unique RNP entity distinct from neuronal hStaufen and/or fragile X mental retardation protein granules, processing bodies, and stress granules. Granules were 100-300 nm in diameter and consisted of IMPs, 40 S ribosomal subunits, shuttling heterologous nuclear RNPs, poly(A)-binding proteins, and mRNAs. Moreover granules contained CBP80 and factors belonging to the exon junction complex and lacked eIF4E, eIF4G, and 60 S ribosomal subunits, indicating that embodied mRNAs are not translated. Granules embodied mRNAs corresponding to about 3% of the human embryonic kidney 293 mRNA transcriptome. Messenger RNAs encoding proteins participating in the secretory pathway and endoplasmic reticulum-associated quality control, as well as ubiquitin-dependent metabolism, were enriched in the granules, reinforcing the concept of RNP granules as post-transcriptional operons.

Original language	English
Journal	Molecular and Cellular Proteomics
Volume	6
Issue number	5
Pages (from-to)	798-811
Number of pages	13
ISSN	1535-9476
DOIs	https://doi.org/10.1074/mcp.M600346-MCP200
Publication status	Published - 2007

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@article{cabb7eb0953a11dd86a6000ea68e967b,

title = "Molecular composition of IMP1 ribonucleoprotein granules.",

abstract = "Localized mRNAs are transported to sites of local protein synthesis in large ribonucleoprotein (RNP) granules, but their molecular composition is incompletely understood. Insulin-like growth factor II mRNA-binding protein (IMP) zip code-binding proteins participate in mRNA localization, and in motile cells IMP-containing granules are dispersed around the nucleus and in cellular protrusions. We isolated the IMP1-containing RNP granules and found that they represent a unique RNP entity distinct from neuronal hStaufen and/or fragile X mental retardation protein granules, processing bodies, and stress granules. Granules were 100-300 nm in diameter and consisted of IMPs, 40 S ribosomal subunits, shuttling heterologous nuclear RNPs, poly(A)-binding proteins, and mRNAs. Moreover granules contained CBP80 and factors belonging to the exon junction complex and lacked eIF4E, eIF4G, and 60 S ribosomal subunits, indicating that embodied mRNAs are not translated. Granules embodied mRNAs corresponding to about 3% of the human embryonic kidney 293 mRNA transcriptome. Messenger RNAs encoding proteins participating in the secretory pathway and endoplasmic reticulum-associated quality control, as well as ubiquitin-dependent metabolism, were enriched in the granules, reinforcing the concept of RNP granules as post-transcriptional operons.",

author = "Lars J{\o}nson and Jonas Vikesaa and Anders Krogh and Nielsen, {Lars K} and Hansen, {Thomas vO} and Rehannah Borup and Johnsen, {Anders H} and Jan Christiansen and Nielsen, {Finn C}",

note = "Keywords: Cell Line; Cytoskeletal Proteins; Fragile X Mental Retardation Protein; Humans; Metabolic Networks and Pathways; Microscopy, Atomic Force; Proteomics; RNA, Messenger; RNA-Binding Proteins; Ribonucleoproteins",

year = "2007",

doi = "10.1074/mcp.M600346-MCP200",

language = "English",

volume = "6",

pages = "798--811",

journal = "Molecular and Cellular Proteomics",

issn = "1535-9476",

publisher = "American Society for Biochemistry and Molecular Biology",

number = "5",

}

TY - JOUR

T1 - Molecular composition of IMP1 ribonucleoprotein granules.

AU - Jønson, Lars

AU - Vikesaa, Jonas

AU - Krogh, Anders

AU - Nielsen, Lars K

AU - Hansen, Thomas vO

AU - Borup, Rehannah

AU - Johnsen, Anders H

AU - Christiansen, Jan

AU - Nielsen, Finn C

N1 - Keywords: Cell Line; Cytoskeletal Proteins; Fragile X Mental Retardation Protein; Humans; Metabolic Networks and Pathways; Microscopy, Atomic Force; Proteomics; RNA, Messenger; RNA-Binding Proteins; Ribonucleoproteins

PY - 2007

Y1 - 2007

N2 - Localized mRNAs are transported to sites of local protein synthesis in large ribonucleoprotein (RNP) granules, but their molecular composition is incompletely understood. Insulin-like growth factor II mRNA-binding protein (IMP) zip code-binding proteins participate in mRNA localization, and in motile cells IMP-containing granules are dispersed around the nucleus and in cellular protrusions. We isolated the IMP1-containing RNP granules and found that they represent a unique RNP entity distinct from neuronal hStaufen and/or fragile X mental retardation protein granules, processing bodies, and stress granules. Granules were 100-300 nm in diameter and consisted of IMPs, 40 S ribosomal subunits, shuttling heterologous nuclear RNPs, poly(A)-binding proteins, and mRNAs. Moreover granules contained CBP80 and factors belonging to the exon junction complex and lacked eIF4E, eIF4G, and 60 S ribosomal subunits, indicating that embodied mRNAs are not translated. Granules embodied mRNAs corresponding to about 3% of the human embryonic kidney 293 mRNA transcriptome. Messenger RNAs encoding proteins participating in the secretory pathway and endoplasmic reticulum-associated quality control, as well as ubiquitin-dependent metabolism, were enriched in the granules, reinforcing the concept of RNP granules as post-transcriptional operons.

AB - Localized mRNAs are transported to sites of local protein synthesis in large ribonucleoprotein (RNP) granules, but their molecular composition is incompletely understood. Insulin-like growth factor II mRNA-binding protein (IMP) zip code-binding proteins participate in mRNA localization, and in motile cells IMP-containing granules are dispersed around the nucleus and in cellular protrusions. We isolated the IMP1-containing RNP granules and found that they represent a unique RNP entity distinct from neuronal hStaufen and/or fragile X mental retardation protein granules, processing bodies, and stress granules. Granules were 100-300 nm in diameter and consisted of IMPs, 40 S ribosomal subunits, shuttling heterologous nuclear RNPs, poly(A)-binding proteins, and mRNAs. Moreover granules contained CBP80 and factors belonging to the exon junction complex and lacked eIF4E, eIF4G, and 60 S ribosomal subunits, indicating that embodied mRNAs are not translated. Granules embodied mRNAs corresponding to about 3% of the human embryonic kidney 293 mRNA transcriptome. Messenger RNAs encoding proteins participating in the secretory pathway and endoplasmic reticulum-associated quality control, as well as ubiquitin-dependent metabolism, were enriched in the granules, reinforcing the concept of RNP granules as post-transcriptional operons.

U2 - 10.1074/mcp.M600346-MCP200

DO - 10.1074/mcp.M600346-MCP200

M3 - Journal article

C2 - 17289661

SN - 1535-9476

VL - 6

SP - 798

EP - 811

JO - Molecular and Cellular Proteomics

JF - Molecular and Cellular Proteomics

IS - 5

ER -

Molecular composition of IMP1 ribonucleoprotein granules.

Abstract

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