Molecular comparison of cultivable protozoa from a pristine and a polycyclic aromatic hydrocarbon polluted site

E Lara; C Berney; Flemming Ekelund; H Harms; A Chatzinotas

doi:10.1016/j.soilbio.2006.06.017

Molecular comparison of cultivable protozoa from a pristine and a polycyclic aromatic hydrocarbon polluted site

E Lara, C Berney, Flemming Ekelund, H Harms, A Chatzinotas

Terrestrial Ecology

43 Citations (Scopus)

Abstract

We compared the abundance and diversity of cultivable protozoa (flagellates and amoebae) in a polycyclic aromatic hydrocarbon (PAH) polluted soil and an unpolluted control, by isolating and cultivating clonal strains. The number of cultivable protozoa was higher in the polluted soil; however, the polluted soil displayed an impoverished community, dominated by certain taxa, such as Acanthamoeba sp. We isolated a total of 31 protozoan strains to characterize them morphologically and by 18S rRNA gene sequence analysis. This approach, i.e. combining morphological and molecular information had the advantage of providing quantitative data, information on morphology but also an accurate positioning of the isolates in 18S rRNA trees.

Original language	English
Journal	Soil Biology & Biochemistry
Volume	39
Issue number	1
Pages (from-to)	139–148
ISSN	0038-0717
DOIs	https://doi.org/10.1016/j.soilbio.2006.06.017
Publication status	Published - 2007

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title = "Molecular comparison of cultivable protozoa from a pristine and a polycyclic aromatic hydrocarbon polluted site",

abstract = "We compared the abundance and diversity of cultivable protozoa (flagellates and amoebae) in a polycyclic aromatic hydrocarbon (PAH) polluted soil and an unpolluted control, by isolating and cultivating clonal strains. The number of cultivable protozoa was higher in the polluted soil; however, the polluted soil displayed an impoverished community, dominated by certain taxa, such as Acanthamoeba sp. We isolated a total of 31 protozoan strains to characterize them morphologically and by 18S rRNA gene sequence analysis. This approach, i.e. combining morphological and molecular information had the advantage of providing quantitative data, information on morphology but also an accurate positioning of the isolates in 18S rRNA trees.",

author = "E Lara and C Berney and Flemming Ekelund and H Harms and A Chatzinotas",

note = "Keywords: 18S rRNA; Naked amoebae; Flagellates; Cultivable diversity; PAH polluted soil",

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T1 - Molecular comparison of cultivable protozoa from a pristine and a polycyclic aromatic hydrocarbon polluted site

AU - Lara, E

AU - Berney, C

AU - Ekelund, Flemming

AU - Harms, H

AU - Chatzinotas, A

N1 - Keywords: 18S rRNA; Naked amoebae; Flagellates; Cultivable diversity; PAH polluted soil

PY - 2007

Y1 - 2007

N2 - We compared the abundance and diversity of cultivable protozoa (flagellates and amoebae) in a polycyclic aromatic hydrocarbon (PAH) polluted soil and an unpolluted control, by isolating and cultivating clonal strains. The number of cultivable protozoa was higher in the polluted soil; however, the polluted soil displayed an impoverished community, dominated by certain taxa, such as Acanthamoeba sp. We isolated a total of 31 protozoan strains to characterize them morphologically and by 18S rRNA gene sequence analysis. This approach, i.e. combining morphological and molecular information had the advantage of providing quantitative data, information on morphology but also an accurate positioning of the isolates in 18S rRNA trees.

AB - We compared the abundance and diversity of cultivable protozoa (flagellates and amoebae) in a polycyclic aromatic hydrocarbon (PAH) polluted soil and an unpolluted control, by isolating and cultivating clonal strains. The number of cultivable protozoa was higher in the polluted soil; however, the polluted soil displayed an impoverished community, dominated by certain taxa, such as Acanthamoeba sp. We isolated a total of 31 protozoan strains to characterize them morphologically and by 18S rRNA gene sequence analysis. This approach, i.e. combining morphological and molecular information had the advantage of providing quantitative data, information on morphology but also an accurate positioning of the isolates in 18S rRNA trees.

U2 - 10.1016/j.soilbio.2006.06.017

DO - 10.1016/j.soilbio.2006.06.017

M3 - Journal article

SN - 0038-0717

VL - 39

SP - 139

EP - 148

JO - Soil Biology & Biochemistry

JF - Soil Biology & Biochemistry

IS - 1

ER -

Molecular comparison of cultivable protozoa from a pristine and a polycyclic aromatic hydrocarbon polluted site

Abstract

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